Paper1: Microbial Cultures Flashcards
What antiseptic techniques can be used to avoid contaminated?
Describe how you could investigate the effect of antibiotics on bacterial growth using zone of inhibition?
How you measure zone of inhibition?
Why should you not completely seal Agar plate!
To allow oxygen to enter the Agar plate, preventing growth of harmful anaerobic bacteria.
Why is it necessary to measure diameter Of zone of inhibition twice?
Clear zones are not always uniform - taking more than one measurement allows a mean diameter to be calculated
What equation is used to calculate area of clear zones?
What is a source of error in this practical?
The area of the clear zone may be irregular and it’s is difficult to determine a suitable width to be recorded
What is a source of error in this practical?
The area of the clear zone may be irregular and it’s is difficult to determine a suitable width to be recorded
