Paper studying Ebstein-Barr Virus Flashcards
1
Q
What is being studied in this paper?
A
Factors causing binding of Origin Recognition Complex in Epstein-Barr as a model for eukaryotic cells
2
Q
What is the primary protein of interest? What RNA does EBNA1 prefer?
A
EBNA1: which is proposed to bind to DNA;
G-rich oligonucleotides
3
Q
What are the two RGG motifs? Why are they called this?
A
LR1 and LR2; they are called this because of their similarity to a family of RNA-binding proteins.
4
Q
How was the interaction studied and proposed?
A
Immunoprecipitation. Treatment with RNase meant that the ORC didn’t precipitate together. DNase didn’t disrupt EBNA1 ORC complex
5
Q
How does concentration of LR1 and LR2 affect ORC recruitment?
A
Exponentially.
6
Q
Cells used?
A
Hela nuclear extracts
