Paper 2 extra information (Ensenberger et al.)

Question 1

• Powerplex 6C fusion system can be used for direct amplification

Other kit validations…
* QIAGEN investigator 24 plex QS kit and investigator: 6-dye multiplex assay that contains all markers of expanded 23 CODIS core loci, designed for purified DNA for casework and reference samples, robust and reliable for forensic DNA typing in casework analysis and databasing

• Globalfiler express PCR amplification Kit: 6-dye multiple and can be used for direct amplification also
• Ampflstr identifiler: direct PCR amplification kit, STR multiplex assay, human specific apart from primates which gave partial profiles

Direct amplification
* NO prior extraction, purification or quantification
* Max DNA obtained than would with above steps
* Minimises error and contamination risk
* reduces time and cost
* But could lead to more complex mixtures