pakc Flashcards
What are the two parts of the pentose phosphate pathway?
Oxidative and non-oxidative
What happens in the oxidative part of PPP?
NADPH is formed from the first reaction
And NADPH and CO2 is formed in the second reaction
What happens in the non-oxidative of PPP?
Transfer of Carbon groups between sugars
What is the compound that we start PPP with?
Glucose 6-phosphate
What is the enzyme that is used to form NADPH in the first reaction of the oxidative part of PPP?
Glucose 6-phosphate dehydrogenase
What regulates glucose 6-phosphate dehydrogenase?
NADPH
What is the enzyme that is required for the second (non-oxidative) part of PPP?
Transketolase
(Bc you are transferring ketone groups)
how does glycolsis and PPP help each other?
If ribose 5-phosphate is accumlated, the reaction will shift towards forming the sugars which glycolsis also uses.
If ribose 5-phosphate is deficent, the reaction will shift from the sugars that glycolsis uses to ribose 5-phosphate
This happens bc both glycolsis and PPP have multiple sugars in common.
What does transketolase require?
It requrires thimine pyrophosphate
What are the things that NADPH forms in reductive biosynthesis?
What is requried for the respiratory burst?
NADPH
How does NADPH act as an antioxidant?
By a reaction between glutathione disulfide and NADPH in the presence of glutathione reductase which forms glutathione which is an antioxidant
Why does G6PD deficency cause hemolytic anemia?
Because of the fact that NADPH won’t form bc of the G6PD defciency which will cause the cell to be unable to protect it self from free radicals leading to cell death.
What are the triggers for free radical formation in G6PD deficeny?
What are classical findings of G6PD defecincy on RBCs?
Heinz bodies and bite cells
What is the test that is used to diagnoss G6PD defeciency?
Fluroscent spot test (FST)
A sample of blood is placed under uv light which should shine green if G6PD is normal.
If no green is seen, then G6PD is deficent.
