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INTRODUCTION TO TISSUE PROCESSING > PAGE 2 > Flashcards

PAGE 2 Flashcards

1
Q

ADVANTAGES OF FRESH TISSUE EXAMINATION

A

✓ Observation of physiologic processes or protoplasmic activities (motion, mitosis,
phagocytosis and pinocytosis)
✓ Relatively simple and easy to perform

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2
Q

DISADVANTAGES OF FRESH TISSUE EXAMINATION

A

✓ Limited use

✓ Liable to develop changes observed after death (putrefaction and autolysis)

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3
Q

METHODS FOR FRESH TISSUE EXAMINATION

A
  1. TEASING
  2. SQUASH
  3. SMEARING
  4. FROZEN SECTION
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4
Q

METHODS FOR SMEARING

A
  1. STREAKING
  2. SPREADING
  3. PULL-APART
  4. TOUCH PREPARATION
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5
Q

OTHER TERM FOR TEASING

A

DISSOCIATION

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6
Q

Dissection or separation of tissue components in NSS or Ringer’s solution

A

TEASING

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7
Q

Examined as stained or unstained

A

TEASING

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8
Q

Anatomical relationship is destroyed

A

TEASING

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9
Q

SIZE OF SPECIMEN FOR TEASING

A

1 MM

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10
Q

Tissue (<1mm) is sandwiched between two slides

A

SQUASH

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11
Q

SIZE OF SPECIMEN FOR SQUASH

A

LESS THAN 1 MM

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12
Q

Stain is applied on one side of the slide and allowed to spread via capillary action

A

SQUASH

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13
Q

for cytological studies, especially for the diagnosis of cancer

A

SMEARING

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14
Q

for sections or sediments

A

SMEARING

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15
Q

SMEARING IS PERFORMED USING WHAT MATERIALS

A
  1. WIRE LOOP
  2. APPLICATOR STICK
  3. ANOTHER SLIDE
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16
Q

Uniform distribution in a direct or zigzag manner

A

STREAKING

17
Q

Thick or mucoid specimens

A

SPREADING

18
Q

Teasing on a slide

A

SPREADING

19
Q

PROCEDURES THAT MAINTAIN INTERCELLULAR RELATIONSHIP

A
  1. SPREADING

2. TOUCH PREPARATION

20
Q

For the preparation of blood and bone marrow smears

A

PULL-APART

21
Q

One side of a slide is allowed to touch a surface of the sample

A

TOUCH PREPARATION

22
Q

Prepared using freezing microtome or cryostat

A

FROZEN SECTION

23
Q

For rapid diagnosis

A

FROZEN SECTION

24
Q

For delicate specimens

A

FROZEN SECTIONS

25
Q

INITIAL STEPS IN TISSUE PROCESSING

A

A. Specimen Accessioning/Identification
B. Gross Examination and Sampling
C. Tissue Processing

26
Q

TASKS FOR Specimen Accessioning/Identification

A

➢ Performed by the medical technologist/INTERN
➢ Check label and request form
➢ The specimen is given a label (numeric or alpha-numeric) which allows
easy accessioning/identification
➢ Request form should have a provisional diagnosis and brief clinical details

27
Q

TASKS FOR Gross Examination and Sampling

A

➢ Performed by the pathologist/RESIDENT PATHOLOGIST
➢ Describing the sample macroscopically
➢ Weight and dimensions of the sample are determined

INTRODUCTION TO TISSUE PROCESSING (5 decks)

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