PAGE 2 Flashcards
ADVANTAGES OF FRESH TISSUE EXAMINATION
✓ Observation of physiologic processes or protoplasmic activities (motion, mitosis,
phagocytosis and pinocytosis)
✓ Relatively simple and easy to perform
DISADVANTAGES OF FRESH TISSUE EXAMINATION
✓ Limited use
✓ Liable to develop changes observed after death (putrefaction and autolysis)
METHODS FOR FRESH TISSUE EXAMINATION
- TEASING
- SQUASH
- SMEARING
- FROZEN SECTION
METHODS FOR SMEARING
- STREAKING
- SPREADING
- PULL-APART
- TOUCH PREPARATION
OTHER TERM FOR TEASING
DISSOCIATION
Dissection or separation of tissue components in NSS or Ringer’s solution
TEASING
Examined as stained or unstained
TEASING
Anatomical relationship is destroyed
TEASING
SIZE OF SPECIMEN FOR TEASING
1 MM
Tissue (<1mm) is sandwiched between two slides
SQUASH
SIZE OF SPECIMEN FOR SQUASH
LESS THAN 1 MM
Stain is applied on one side of the slide and allowed to spread via capillary action
SQUASH
for cytological studies, especially for the diagnosis of cancer
SMEARING
for sections or sediments
SMEARING
SMEARING IS PERFORMED USING WHAT MATERIALS
- WIRE LOOP
- APPLICATOR STICK
- ANOTHER SLIDE
Uniform distribution in a direct or zigzag manner
STREAKING
Thick or mucoid specimens
SPREADING
Teasing on a slide
SPREADING
PROCEDURES THAT MAINTAIN INTERCELLULAR RELATIONSHIP
- SPREADING
2. TOUCH PREPARATION
For the preparation of blood and bone marrow smears
PULL-APART
One side of a slide is allowed to touch a surface of the sample
TOUCH PREPARATION
Prepared using freezing microtome or cryostat
FROZEN SECTION
For rapid diagnosis
FROZEN SECTION
For delicate specimens
FROZEN SECTIONS
INITIAL STEPS IN TISSUE PROCESSING
A. Specimen Accessioning/Identification
B. Gross Examination and Sampling
C. Tissue Processing
TASKS FOR Specimen Accessioning/Identification
➢ Performed by the medical technologist/INTERN
➢ Check label and request form
➢ The specimen is given a label (numeric or alpha-numeric) which allows
easy accessioning/identification
➢ Request form should have a provisional diagnosis and brief clinical details
TASKS FOR Gross Examination and Sampling
➢ Performed by the pathologist/RESIDENT PATHOLOGIST
➢ Describing the sample macroscopically
➢ Weight and dimensions of the sample are determined
