P1: Flow cytometry Flashcards by A W | Brainscape

Q

What is the effect of nocodazole treatment?

A

Cell cycle inhibitor

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Q

How does tryptan demonstrate cell viability?

A

Blue exclusion dye
Excluded by live cells with an intact plasma membrane
Taken up by dead or dying cells

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Q

What is the function of propidium iodide staining?

A

Fluorescent DNA binding dye
Able to determine DNA content of cells after fixation with ethanol
Stains red
Amount of DNA elucidates cell cycle stage e.g. G2 (after S phase) is expected to have twice as much DNA and therefore red fluorescence as G1

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