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Seminar 1 > (P) Versatrek Training > Flashcards

(P) Versatrek Training Flashcards

video lecture-based

1
Q

TOF. VERSATREK is an automated blood culture system.

A

T

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2
Q

versaTREK is manufactured by?

A

ThermoFischer Scientific

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3
Q

PRINCIPLES OF AUTOMATED BLOOD CULTURE MACHINE (3)

A

● Colorimetric Technology
● Fluoreescent Technology
● Pressure Change Detection

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4
Q
  • organisms grow in media and produce carbon dioxide (CO2)
  • CO2 passes through semi-permeable membrane located at the bottom of the blood culture bottle
  • sensor changes from green to yellow
  • Positive butt of culture bottle will be detected by the blood culture analyzer
A

COLORIMETRIC TECHNOLOGY

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5
Q

COLORIMETRIC TECHNOLOGY

It is based on what chemistry?

A

Growth chemistry

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6
Q

COLORIMETRIC TECHNOLOGY

Indicators:

  1. Green
  2. Yellow
A
  • Negative
  • Positive
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7
Q
  1. Organism metabolic activity releases CO2
  2. Carbon dioxide reacts with dye in vial sensor located at the bottom of the blood culture bottle
  3. LED-activated fluorescent material in sensor.
  4. Photodetector reads fluorescence
  5. Raw data from detector is analyzed by row board
  6. And positivity analysis is performed
  7. Positive vial is announced by audible alert, on screen indications, and the Positive Vial system indicator.
A

FLUORESCENT TECHNOLOGY

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8
Q

FLUORESCENT TECHNOLOGY

This reacts with dye in vial sensor located at the
bottom of the blood culture bottle?

A

CO2

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9
Q

FLUORESCENT TECHNOLOGY

modulated by dye in the sensor

A

Fluorescence

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10
Q

FLUORESCENT TECHNOLOGY

This reads the fluorescence

A

Photodetector

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11
Q

FLUORESCENT TECHNOLOGY

Positive vial is announced by audible alert, on screen
indications, and the?

A

Positive Vial system indicator

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12
Q
  • This principle is utilized by VersaTREK Technology
  • During metabolism, microorganisms produce and/or consume gases (CO2, H2, O2, N2, etc.)
A

PRESSURE CHANGE DETECTION

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13
Q

PRESSURE CHANGE DETECTION

They measure these changes in pressure in the
headspace of inoculated bottles over time to determine positivity

A

VersaTREK

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14
Q

TOF. Production decreases pressure while consumption increases.

A

F (reverse)

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15
Q

PRESSURE CHANGE DETECTION

No CO2 limitations in VersaTREK’s TI

itz a technology

A

Comprehensive Detection Technology (CDT)

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16
Q

Consumption of oxygen is decreased in pressure followed by constant pressure which gradually decreases, then the rapid or sharp increase of pressure that is now called

A

carbon dioxide production

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17
Q

TOF. When microbes may not produce a sufficient amount of CO2, this can become a problem for colorimetric or fluorescence technology.

A

T

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18
Q
  • Connector provides the link (via a recessed probe) for headspace pressure measurement
  • Allows dissipation of gases produced during metabolism
A

VERSATREK SAFETY - CONNECTOR

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19
Q

VERSATREK SAFETY - CONNECTOR

a component in the connector prevents
aerosol escape

A

Hydrophobic membrane

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20
Q
  • A major advantage of VersaTREK Technology by continuously stirring the culture media in the bottle
  • Proven to be 2 hours faster than the stir bar
A

VORTEXING

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21
Q

Author of Preliminary Development and Verification of a New Agitation Method for the ESP Culture Technology

A

N.M Sullican and D. Fett

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22
Q

ESP Culture Technology

TOF. These data show that the stir bar agitation method is a very effective method when used with the ESP technology

23
Q

ORGANISMS THAT SIGNAL ON GAS PRODUCTION

A

● Staphylococcus aureus
● Staphylococcus epidermidis
● Staphylococcus pneumoniae
● Enterococcus faecalis
● Escherichia coli
● Candida albicans
● Klebsiella pneumoniae

SSSEECK

24
Q

ORGANISMS THAT SIGNAL ON GAS CONSUMPTION

A

● Brucella suis
● Helicobacter spp.
● Nocardia spp.
● Campylobacter spp.
● Brevundimonas vesicularis
● Rhodococcus equi
● Trichoporum beigelli

25
Advantages of VERSATREK TECHNOLOGY | 4
1. CDT 2. NO LOW-CO2 LIMITATIONS 3. NO HIGH WBC LIMITATIONS 4. FOUR-IN-ONE SYSTEM
26
* Competitive systems rely on CO2 production * Many fastidious organisms do not produce CO2 * Maximum Recovery and Minimum TTD for Campylobacter spp., Neisseria spp., Brucella spp., etc.
NO LOW-CO2 LIMITATIONS
27
● Pressure-based system ● Extensive algorithms distinguish bacterial growth from WBC metabolism up to 100,000 ● Reduction of false positives and workflow disruptions
NO HIGH WBC LIMITATIONS
28
VersaTREK is the only one system with 4 US FDA-cleared applications on 1 platform: ○ Blood Culture (0.1-10 mL) ○ Sterile body fluids ○ Mycobacteria detection ○ Mycobacterium TB susceptibility testing
FOUR-IN-ONE SYSTEM
29
blood-to-broth dilution ratio of most blood culture bottles in the market
1:4
30
blood-to-broth dilution ratio for Thermo Fisher Scientific
1:8
31
# VERSATREK BOTTLES What is diluted to neutralize and increase the sensitivity of detection for the cause of infection
VERSATREK BOTTLES
32
BENEFITS OF VERSATREK ➢ Enhance fastidious detection A. Vortexing B. Gas consumer/producer C. Lower SPS D. Blood-to-broth ratio (1:8) Dilution
B
33
BENEFITS OF VERSATREK ➢ Faster time to detection A. Vortexing B. Gas consumer/producer C. Lower SPS D. Blood-to-broth ratio (1:8) Dilution | 2 answers
A and B
34
BENEFITS OF VERSATREK ➢ Less inhibitor, superior to recover fastidious organisms A. Vortexing B. Gas consumer/producer C. Lower SPS D. Blood-to-broth ratio (1:8) Dilution
C
35
BENEFITS OF VERSATREK ➢ Easier stock management A. ONE bottle for all-purpose B. Gas consumer/producer C. Lower SPS D. Blood-to-broth ratio (1:8) Dilution
A
36
BENEFITS OF VERSATREK ➢ Dilutes antibiotics and serum cofactors A. ONE bottle for all-purpose B. Gas consumer/producer C. Lower SPS D. Blood-to-broth ratio (1:8) Dilution
D
37
BENEFITS OF VERSATREK ➢ Detection even for low blood volume collection A. ONE bottle for all-purpose B. FDA-cleared blood volume C. FDA cleared for 4 platforms D. Blood-to-broth ratio (1:8) Dilution
B
38
BENEFITS OF VERSATREK ➢ Comprehensive organisms detect from blood, sterile body, Mycobacteria ID, Mycobacteria AST A. ONE bottle for all-purpose B. FDA-cleared blood volume C. FDA cleared for 4 platforms D. Blood-to-broth ratio (1:8) Dilution
C
39
# GUIDELINES FOR BLOOD CULTURE COLLECTION No Blood sets/episode * USA CLSI * USA ASM * Europe ESCMID * Australia
* USE CLSI, USA ASM, Europe ESCMID - 2-3 * Autralia - 2
40
# GUIDELINES FOR BLOOD CULTURE COLLECTION paaral nalang iba sori dcrammer
thx
41
Number of Blood Culture Set and state their % Detection * 1 set (20 mL) * 2 sets (40 mL) * 3 sets (60 mL) * 4 sets (80 mL)
* 65% * 80% * 96% * Nearly 100%
42
# BLOOD CULTURE COLLECTION PROCEDURE paaral nalang po thx
mwah mwah * skin prep * bottle prep * VENIPUNCTURE AND BOTTLE INOCULATION
43
VENIPUNCTURE AND BOTTLE INOCULATION | 3 options
* NEEDLE AND SYRINGE * BLOOD COLLECTION DEVICE
44
When your blood culture analyzer signals positive, you unload the positive culture bottle and perform the following: | 4
* gram stain * isolation * ID/AST * Reporting
45
# POSITIVE BLOOD CULTURE WORKUP ■ Subculture to media ■ Isolation of causative pathogen ■ Blood Agar ■ Additional Media: (MacConkey Agar, Chocolate Agar, Anaerobic Blood Agar)
ISOLATION
46
# POSITIVE BLOOD CULTURE WORKUP ■ Work-up of culture ■ Biochemical ID kit ■ Mueller Hinton Agar/Kirby Bauer ■ MICE ■ Sensititre System (ThermoFisher Scientific)
ID/AST
47
# REPORTING No growth
● No growth at 24 hours ● No growth at 48 hours
48
# PRELIMINARY RESULT ● Positive Blood Culture | perform what
○ Gram stain result ○ Preliminary ID ■ Gram-positive cocci ■ Gram-positive bacilli ■ Gram-negative bacilli
49
# FINAL RESULT No growth after the length of incubation
○ After 5 days (according to CLSI) ○ After 7 days (according to ASM)
50
# FINAL RESULT Positive Blood Culture
○ Final Gram Stain Result ○ Final ID ○ Final AST
51
● Alternative to automated blood culture system ● Also based on pressure change
MANUAL BLOOD CULTURE
52
MANUAL BLOOD CULTURE | 1
SIGNAL BLOOD CULTURE SYSTEM
53
Four tests in one platform
○ Blood (Culture) ○ Sterile (Body Fluids) ○ Mycobacteria (Detection) ○ MTB Susceptibility
54

Seminar 1 (9 decks)

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