P/G Flashcards
ECM composed of
Proteoglycans
Proteoglycans composed of
Glycoasminoglycans (GAGs) linked by central protein
Bottle brush formation
GAGs composed of
Negative charged sugars
Repeating disaccharide units = position 1 acidic sugar / position 2 amino sugar
Name GAG properties
Strong negative charge
Binds lots of water = gel like matrix
Reacts to compression with expel water
Reacts to relaxation with absorbing water
Most abundant GAG
Chondroitons (sulfated pos 4/6)
Chondroitons are found in
BLAC
Bone, Ligaments, Aorta, Carltilage
Keratan Sulfate I
GAG
Found in Cornea
Keratan Sulfate II
GAG
Found in CBC
Connective tissue, bone, cartilage
Dermatan Sulfate
GAG
Found in Skin, Blood, Heart valve
Heparan Sulfate
GAG
Found in BM, cell surfaces for recognition
Heparin
GAG
Anticoagulant
GAG with most sulfates and most Negative charge
Bottle Brush Structure
GAGs = neg charged
GAGs = hydrated
so they repel
Linked to central core protein
Make up proteoglycans which are connected to hyaluronic acid
Synthesis of Glucoronic acid
Glucose to UDP glucose
UDP glucose oxidized to UDP glucoranic acid
Synthesis of Amino sugars
Glucose to glucosamine 6-P
Glucosamine 6-P to UDP glucosamine
Hyaluronic acid
Not sulfated
Not covalently bond to protein
Made in ECM
Provides central stand for proteoglycan aggs
Hyaluronic acid found in
Hyaluronic acid facilitates cell migration in
Vitreous humor eye, joint fluid, cartilage, loose connective tissue
Embryogenesis
Morphogenesis
Wound repair
Glycoproteins
Sugars with O link = serine or threonine
Sugars with N link = asparagine
Mucins
Glycoproteins rich with carbs
O glycosylated
Blood Types
O - no link to GalH
A - link to GalNAc
B - link to galactose
AB - mixture
N Glycosylation of Proteins
Mann rich precursor bound to dolichol-pp
Precursor given to all proteins then modified later
Sugar N link via Argenine
Transport of enzymes from Golgi to Lysosome
N Linked glycoproteins receive mannose 6-P marker via phoshpotransferase
Golgi has mannose 6-P receptors = binds lysosomal enzymes = packaged into vesicles = transport to lysosomes
I Cell Disease
Lacks phospotransferase
Lack of mannose 6-P marker in lysosomes
No lysosomal enzymes in lysosome
lack of degradation = leads to accumulation of inclusion bodies (I bodies)
