Oxygenic Photosynthesis Flashcards

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1
Q

what is photosynthesis

A

conversion of light energy to chemical energy and reducing power

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2
Q

what does NADP+ reductase form

A

NADPH

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3
Q

what do thylakoids stack up to form

A

grana (singular = granum)

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4
Q

what is the chloroplast equivalent of the cytosol called

A

stroma

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5
Q

why are chloroplasts green

A

they reflect the green wavelength of photosynthetically active radiation

chlorophyll a and b absorb and use blue and red light so all that is reflected back is green

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6
Q

what allows chlorophyll to associate with the lipid thylakoid membrane

A

lipophilic isoprenoid chain

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7
Q

what part of chlorophyll absorbs light energy
how is this possible

A

pyrrole ring structure
energy delocalises electrons which then exist in a cloud around the ring and are not bound to one atom

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8
Q

where do light energy harvesting processes occur

A

across thylakoid membrane

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9
Q

what complexes are responsible for catching light

A

photosystems

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10
Q

how are electrons supplied to photosystem II

A

oxidising water to oxygen

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11
Q

photosynthesis summary (5 steps)

A

1) excitation of antenna chlorophyll
2) transfer of energy to reaction centre
3) primary charge separation event
4) electron transport chain forms electrochemical gradient to drive ATP synthesis and form NADPH
5) H2O oxidised to replenish reaction centre electron

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12
Q

what is chlorophyll

A

a specialized pigment embedded in the thylakoid membrane which absorbs light energy

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13
Q

how does chlorophyll absorb light

A

energy of photoexcitation delocalises electrons

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14
Q

what is the primary charge separation

A

the conversion of energy from physical to chemical state

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15
Q

describe the thylakoid electron transport chain

A

light is absorbed at the P680 photosystem II and electrons are passed to plastoquinone
energy difference transports 4 protons across the membrane
light is absorbed at P700 photosystem I and electrons are used by ferredoxin to turn NADP+ to NADPH

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16
Q

how are electrons of each photosystem replenished

A

PSI replenished by plastocyanin (from ETC)
PSII replenished by liberation of electrons from water producing O2 and 4 protons

17
Q

how do NADPH and ATP go on to be used

A

in the Calvin cycle to make sugars and fatty acids etc

18
Q

what enzyme splits water to replenish electrons lost by PSII
what is the bi-product

A

4 manganese enzyme
oxygen

19
Q

what can happen if there is too much light absorbed by photosystems

A

too many electrons
ROS are released and can cause damage
accumulation of protons in lumen

20
Q

how does VDE prevent too many electrons

A

converts violaxanthin to zeaxanthin, ceases electron transport chain and energy is released as heat

21
Q

what is the rate limiting step of PSI

A

NADPH synthesis