Overview Microbiology Flashcards
What is microbiology?
Study of microorganisms or minute living bodies not perceptible to the naked eye.
What are the microorganisms?
Bacteria, Viruses, Protozoa Rickettsiae, Algae
Robert Hooke 1665
An English man
.1st to report on cell theory - that all living things are composed of cells
What is a cell?
The basic functional unit of all living organisms. The human body is made up of 50-100 trillion cells. Cells combine to form tissue & organs. The cell contains the genetic material, DNA (Deoxyribonucleic Acid.)
Anton Van Leeuwenhoek 1673
A Dutch scientist
. 1st to observe and record Bacteria & Protozoa;
Protozoa
One-celled animal
Edward Jenner 1798
English physician
- Developed the 1st vaccine against smallpox
Ignaz Semmelweis 1840
Advocated frequent hand washing between treatment of patients to prevent the spread of infection
Louis Pasteur 1857
Discovered that heating can kill bacteria (pasteurization)
Joseph Lister (1860)
English surgeon
. 1st to advocate aseptic technique to be used in the operating room
. Used phenol as an antiseptic agent
Robert Koch 1876
German physician, proved that bacteria are the cause of disease
. Discovered the organisms causing tuberculosis, anthrax and cholera
. Developed techniques to culture bacteria
. Urged the sterilization of surgical instruments using heat
Hans Gram 1884
Danish physician, developed the Gram staining method that differentiates between gram-positive and gram-negative bacteria
Microscopy
The use of light to magnify objects
Microscope
Is an instrument used to see objects that are too small for the naked eye
Simple Microscope
With a single magnifying lens, capable of approximately 300x magnification
Compound Microscope
Uses a series of lenses for greater magnification; can magnify 1,000-2,000x
Dark Field Microscope
Useful for examining pale or colorless cells; specimen appears light against a dark ground
Fluorescent Microscope
Uses UV light to fluoresce objects
Electron Microscope
Can magnify objects 10,000-100,000x
What are the differences in Eukaryotic & Prokaryotic cells?
. Presence of membrane-bound compartments (such as the nucleus) in eukaryotes
. Prokaryotes - less complex organisms ex. Bacteria
. Eukaryotes - more complex organisms ex. Plant & Animal cells, Protozoa, fungi, algae, human cells
Eukaryotic - Structure
- Cell Membrane (plasma membrane) - the thin membrane that forms the outer boundary of the cell
- Nucleus - the membrane-bound structure inside the cell that contains the genetic material DNA (deoxyribonucleic acid)
- Cytoplasm - the gel-like living fluid in the cell between the cell membrane & nucleus
What contains organelles & define each organelle
The cytoplasm
1. Ribosomes- tiny particles that serve as “protein factories” of the cell making enzymes & other protein compounds
2. Mitochondria- “power plant” of the cell containing enzymes and substances whose reactions release energy to recharge the cells “battery” - the ATP (adenosine triphosphate)
3. Lysosomes - the “digestive bags” of the cell containing enzymes that break apart large food molecules through hydrolysis. Lysosomes digest & destroy microbes that invade the cell.
4. Centrioles- are paired rod-shaped structures that play an important role for moving the chromosomes during cell division.
Eukaryotic- Cell Membrane
The cell membrane is the gateway of the cell that controls the passage of materials in and out of the cell.
. Proteins - in the cell membrane act as receptors that “communicate” chemical messages from hormones that influence the function of the cell
. Carbohydrate - chains on the membrane play a part in identification of the cell as specifically belonging to an individual. This is used during tissuetyping prior to organ transplant.
Eukaryotic- Nucleus
A small spherical structure in the central portion of the cell that is surrounded by the nuclear envelope.
. It is the cell’s command center that controls every organelle in the cytoplasm
. It contains the genetic material, DNA (deoxyribonucleic acid)
Eukaryotic- Cell Extension
- Microvilli- are small finger-like projections of the plasma membrane that increase the absorptive capacity of the cell. Ex. Intestinal microvilli
- Cilia - are extremely fine hair-like extensions on the exposed surface of the cell serving as “antennae” for the cell to sense its surroundings. Ex. Cilia in taste buds
Some cilia are capable of moving as a group in one direction to propel mucus ex. Cilia in respiratory tubes - Flagellum- is a single, long projection of the cell used for movement ex. Flagellum of the sperm
Prokaryotes- Structure 6 total
No true nucleus (not enclosed within a membrane)
1. Bacterial Cell Wall
2. Cell Membrane or Cytoplasmic Membrane
3. Capsule & Slime Layer
4. Cytoplasm
5. Flagella
6. Pili
Bacterial Cell Wall
Rigid, gives shape to the cell, forms a physical barrier to the outside of the cell
Cell Membrane or Cytoplasmic Membrane
Lies immediately within the cell wall
. Selectively permeable, involved in cellular respiration
. Secretes bacterial toxins
Capsule & Slime Layer + Function
Sugar coat (polysaccharides) that surrounds the cell wall
. Capsule - strongly attached to the cell wall
. Slime Layer - loosely attached to the cell wall
Functions of both:
1. Protect the bacteria against desiccation & environment toxins
2. Provide ability of bacteria to move on solid surfaces
3. Allow bacteria to anchor on surface of mucous membrane & teeth
Cytoplasm
Gelatinous material contained within the cell membrane
. Contains materials necessary for cell metabolism- water, nutrients, proteins, enzymes - as well as waste products
Flagella
Long, snake-like appendage that allows bacteria to move about its environment
. Typically found in rod-shaped GRAM Negative bacteria
Salmonella organisms with flagella
Pili + Functions
Hair-like structures typically found in GRAM Negative bacteria
Functions:
1. Site of attachment for particular types of bacteria
2. Venue to transfer genetic material to another cell
3. Provide bacteria like ability to attach to other bacteria or surfaces such as lining of intestine
E.Coli organisms with pili
Endospores
. Stable resting stages that barely metabolize and germinate only when conditions improve
. Organisms (bacteria) convert into endospores when environmental conditions are unfriendly to survival
. A means of bacterial survival wherein the genetic material of the cell is enclosed in several protein coats that are resistant to heat, dry conditions and many chemicals
Vegetative Cells
Cells that are actively growing
Binary Fission
A cell dividing into 2 daughter cells as a way of reproduction
Transport Across Cell Membrane
Movement of substances in and out of the cell membrane is accomplished through 2 transport processes:
1. Passive transport- involves the movement of substances from a high concentration to a low concentration. No cellular energy is required
2. Active transport- involves the movement of substances from a low concentration to a high concentration therefore, cellular energy from ATP is required. Active transport occurs only through living membrane
Principles in Passive Transport Processes. 3 total
- Diffusion- requires big pores for the particles to pass through the cell membrane. Solute as well as water both move from high to low concentration to achieve equilibrium.
- Osmosis- is the diffusion of water (only) through selectively permeable membrane that does not allow solutes to pass through
- Filtration- requires the force of hydrostatic pressure gradient that allows water and diffusible solutes to filter out from the solution with higher hydrostatic pressure to the solution with lower hydrostatic pressure
Diffusion
requires big pores for the particles to pass through the cell membrane. Solute as well as water both move from high to low concentration to achieve equilibrium.
Osmosis
is the diffusion of water (only) through selectively permeable membrane that does not allow solutes to pass through
Filtration
requires the force of hydrostatic pressure gradient that allows water and diffusible solutes to filter out from the solution with higher hydrostatic pressure to the solution with lower hydrostatic pressure
Principles in Active Transport Processes:
Ex. Ion pump, phagocytosis, pinocytosis-movement of substances against a concentration gradient
1. Ion pumps are carriers that move ions against concentration gradients. Pumps may be particular to one ion or may be “couples” to pump 2 ions simultaneously (ex. Na+/k+ pump)
2. Large particles are engulfed during phagocytosis, whereas fluid and dissolved substances are brought into the cell in pinocytosis
Exocytosis
Process by which a cell directs the contents of vesicles out of the cell membrane & into the extracellular space
Autotrophic
Self nourishing, acquire food from inorganic matter (carbon dioxide)
Ex. Plants, non-pathogenic bacteria
Heterotrophic
Cannot make its own food
-acquire food from living or dead organic matter
Ex. Humans, animals, pathogenic bacteria
What is Symbiosis & examples of it?
A relationship between 2 organisms that have close contact with each other; the relationship may be beneficial, harmful or neutral.
1. Mutualism
2. Commensalism
3. Parasitism
4. Pathogenic
5. Antibiosis
6. Neutralism (Independence)
- Mutualism
A close relationship beneficial to both organisms
Ex. Oxpecker feeds on ticks on zebras skin; bird gets food & zebra is provided pest control
Escherichia Coli in human intestines obtain nutrients from the food humans eat. Consequently, it produces Vitamin K that is necessary for blood clotting.
- Commensalism
A relationship between 2 organisms in which 1 organism benefits and the other neither benefits nor is harmed.
ex. Remora gosh & shark. The remora fish feeds in the remains of the sharks food; the shark is not harmed
Ex. Natural bacteria in our skin are provided with a home, yet are not harmful to humans
- Parasitism
One organism is harmed by the presence of the other organism
Ex. Relationship between man & helminths (parasitic intestinal worms)
- Pathogenic
One organism is capable of causing disease in the other.
Pathogen- a disease- causing microorganism
Ex. Streptococcus causes tonsillitis
- Antibiosis
One organism produces substances or causes conditions which are lethal for other organisms
Ex. Relationship between human beings & pathogens that can cause death
Ex. Pseudomonas infection can cause death
- Neutralism (Independence)
No organism benefits or is harmed by the relationship
Ex. Relationship between zebras & wildebeests
Normal Flora
Organisms that normally live in and on the bodies of healthy persons without causing harm.
. Potentially pathogenic as they can cause disease if the hosts defenses are lowered or they can enter through surgical wounds to cause disease