Outcomes 1 and 2 Flashcards
Technique where vein is punctured by needle to withdraw blood or inject material
Venipuncture
Process of entering the vein in order to remove blood
Phlebotomy
Clotted specimens =
Serum
Serum will not contain ____________, since it has been converted to _________ and is part of the clot.
Fibrinogen / Fibrin
In order to form a clot, the following blood constituents are required:
Platelets, calcium, blood clotting factors, fibrinogen (a protein)
Collection tube colours that yield serum:
Red, Gold, (SST), Tiger-topped, Orange (RST)
Un-centrifuged, anticoagulated sample may also be known as:
Whole blood
A centrifuged, anti-coagulated sample will yield:
Plasma
Describe the 3 layers of a centrifuged, anticoagulated sample.
Supernatant is plasma, middle layer is buffy coat (white blood cells and platelets), precipitate is red blood cells
Plasma contains _________ since the anticoagulant prevents it from converting to ______.
Fibrinogen / Fibrin
Collection tube colours that yield plasma:
Lavender (CBC), Light Blue (Coag), Light and Dark Green (Chemistry), Grey
Anticoagulant will remove either the ______ or the _______ from the plasma.
Calcium, thrombin
Plasma or serum appears to be slightly pink to dark red. Red cells have burst. Most specimens are now unsuitable.
Hemolyzed specimen
Plasma or serum appears milky or cloudy. Caused by increase in lipid in specimen, and avoided by collecting “fasting” specimens.
Lipemia
Serum or plasma is golden or deep yellow colour, due to increase of bilirubin.
Icteric
Gauge number possible range
20 - 25 gauge
Most common gauge sizes for adult venipunctures
21 gauge (green) and 22 gauge (black)
SESD
Safety Engineered Sharp Devices
Solid colour Hemoguard lids indicate:
Fully evacuated tube (full draw)
Translucent colour Hemoguard lids indicate:
Partially evacuated tube (low draw)
Evacuated tubes volume ranges from:
1.2mL and 10mL
Well mixed, anticoagulated (EDTA), not centrifuged
Whole blood