Outcomes 1 and 2 Flashcards

1
Q

Technique where vein is punctured by needle to withdraw blood or inject material

A

Venipuncture

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2
Q

Process of entering the vein in order to remove blood

A

Phlebotomy

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3
Q

Clotted specimens =

A

Serum

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4
Q

Serum will not contain ____________, since it has been converted to _________ and is part of the clot.

A

Fibrinogen / Fibrin

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5
Q

In order to form a clot, the following blood constituents are required:

A

Platelets, calcium, blood clotting factors, fibrinogen (a protein)

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6
Q

Collection tube colours that yield serum:

A

Red, Gold, (SST), Tiger-topped, Orange (RST)

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7
Q

Un-centrifuged, anticoagulated sample may also be known as:

A

Whole blood

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8
Q

A centrifuged, anti-coagulated sample will yield:

A

Plasma

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9
Q

Describe the 3 layers of a centrifuged, anticoagulated sample.

A

Supernatant is plasma, middle layer is buffy coat (white blood cells and platelets), precipitate is red blood cells

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10
Q

Plasma contains _________ since the anticoagulant prevents it from converting to ______.

A

Fibrinogen / Fibrin

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11
Q

Collection tube colours that yield plasma:

A

Lavender (CBC), Light Blue (Coag), Light and Dark Green (Chemistry), Grey

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12
Q

Anticoagulant will remove either the ______ or the _______ from the plasma.

A

Calcium, thrombin

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13
Q

Plasma or serum appears to be slightly pink to dark red. Red cells have burst. Most specimens are now unsuitable.

A

Hemolyzed specimen

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14
Q

Plasma or serum appears milky or cloudy. Caused by increase in lipid in specimen, and avoided by collecting “fasting” specimens.

A

Lipemia

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15
Q

Serum or plasma is golden or deep yellow colour, due to increase of bilirubin.

A

Icteric

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16
Q

Gauge number possible range

A

20 - 25 gauge

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17
Q

Most common gauge sizes for adult venipunctures

A

21 gauge (green) and 22 gauge (black)

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18
Q

SESD

A

Safety Engineered Sharp Devices

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19
Q

Solid colour Hemoguard lids indicate:

A

Fully evacuated tube (full draw)

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20
Q

Translucent colour Hemoguard lids indicate:

A

Partially evacuated tube (low draw)

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21
Q

Evacuated tubes volume ranges from:

A

1.2mL and 10mL

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22
Q

Well mixed, anticoagulated (EDTA), not centrifuged

A

Whole blood

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23
Q

anticoagulated and centrifuged to obtain the supernatant, which is

A

Plasma

24
Q

Specimens allowed to clot, and centrifuged to separate the clot (cellular content) from the supernatant, which is:

A

Serum

25
Q

Light blue tube additive

A

Na Citrate

26
Q

Na Citrate tube colour

A

Light Blue

27
Q

Light Green (PST) tube additive

A

Lithium heparin

28
Q

Lithium heparin tube colour

A

light green (PST)

29
Q

Lithium heparin tube colour

A

dark green

30
Q

dark green tube additive

A

lithium heparin

31
Q

sodium heparin tube colour

A

dark green

32
Q

dark green tube additive

A

sodium heparin

33
Q

Lavender tube additve

A

EDTA (Ethylene Diamine Tetra Acetic acid)

34
Q

EDTA tube colour

A

lavender

35
Q

grey tube additive

A

Na fluoride with K oxolate

36
Q

Na fluoride with K oxalate tube colour

A

Grey

37
Q

Plain red tube

A

Usually no additive, sometimes clot activator, serum

38
Q

usually no additive, serum

A

plain red tube

39
Q

SST

A

Serum Separator Tube

40
Q

Gold / SST / Red-Grey “tiger top”

A

barrier gel, serum

41
Q

regular barrier gel

A

gold, sst, tiger top tube

42
Q

orange/RST (Rapid separator tube)

A

gel barrier and thrombin to accelerate clotting, serum

43
Q

barrier gel and clot accelerator

A

orange / rst

44
Q

whole blood or blood culture recommended inversions

A

8

45
Q

citrated plasms (Light blue - Na citrate) recommended inversions

A

3-4

46
Q

Serum in red tube recommended inversions

A

0 for glass, 0-5 for plastic

47
Q

serum in gold (sst) tube recommended in versions

A

5

48
Q

heparinized plasma in green heparin tube recommended inversions

A

8

49
Q

whole blood in lavender (EDTA) tube recommended inversions

A

8

50
Q

oxolate/fluoride plasma in grey tubes recommended inversions

A

8

51
Q

Order of Draw

A
  1. Blood cultures
  2. Light blue (Na citrate)
    3a. Serum in red
    b. Serum in Tiger top sst
    c. serum in gold sst
    4a. Heparin plasma in light green
    b. Heparin plasms in dark green (lithium then sodium)
  3. Lavender EDTA
  4. Grey
52
Q

most desirable vein for venipuncture

A

median cephalic vein

53
Q

second most desirable vein for venipuncture

A

median cubital vein

54
Q

third most desirable vein to use for venipuncture

A

median vein

55
Q

not as desirable veins to use for venipuncture

A

basilic vein (close to nerve and artery) and cephalic vain (small and likes to roll)