Organisation

Question 1

What is a tissiue

Answer 1

A group of cells which work together for a similar function

Question 2

What is an organ

Answer 2

An organ is a group of tissues which together for a similar function

Question 3

What is a cell

Answer 3

Basic building block that all organisms have

Question 4

What is an organ system

Answer 4

An organ system is a group of organs working together

Question 5

Order of organisation

Answer 5

Cell-Tissue-Organ-Organ System

Question 6

What is an enzyme

Answer 6

An enzyme is a biological catalyst which speeds up a certain reaction without being used up and is a big protein molecule

Question 7

What is the term ‘optimum’

Answer 7

Best temperature for enzyme action

Question 8

What is the lock and key theory

Answer 8

Active site of the enzyme is complimentary to the substrate and reacts and breaks down the substrate into smaller molecules

Question 9

What affect Enzyme Action

Answer 9

Temperature affect enzymes- may speed up reactions we don’t want happening

Question 10

What does Amylase break down

Answer 10

Carbohydrates into sugar/glucose

Question 11

Where is Amylase produced

Answer 11

Salivary glands and pancreas

Question 12

Where does Amylase break down carbohydrates

Answer 12

Mouth and small intestine

Question 13

What does protease break down

Answer 13

Proteins to amino acids

Question 14

Where is protease produced

Answer 14

Stomach
Pancreas
Small Intestine

Question 15

Where does Protease break down Proteins

Answer 15

Stomach

Small intestine

Question 16

What does Lipase break down

Answer 16

Lipids to fatty acids and glycerol

Question 17

Where is Lipase produced

Answer 17

Pancreas

Small intestine

Question 18

Where does Lipase break down Fatty acids

Answer 18

Small Intestine

Question 19

How does pH affect enzyme Action

Answer 19

1.Place iodine solution on every spotting tile
Using an electric water bath at 35 degrees
Using a syringe add 2cm3 of amylase solution and 2cm3 of buffered solution of pH 5 into both test tubes
Then place the test tube into the water bath
Using a different syringe add 5cm3 of starch solution to the boiling tube
Mix the contents after labelling the test tube A+B which contains the buffered pH 5 and Amylase
Start stopwatch and every 10 seconds remove 1 drop of the mixture with a plastic pipette. Solution will turn blue black
Continue until the iodine stays orange
repeat with other buffered pH’s

Question 20

What reagent do you test for sugar

Answer 20

Benedict’s solution

Question 21

What reagent do you test for starch

Answer 21

Iodine solution

Question 22

Test for protein

Answer 22

Biuret solution

Question 23

Test for lipids

Answer 23

Ethanol

Question 24

How do you test for starch

Answer 24

Place food sample on the spotting tile
Add few drops of iodine solution
Orange brown-blue black if present
Record all observations in a results table

Question 25

How do you test for sugars

Answer 25

Place a small amount of food sample in a test tube
add approximately 10 drops of benedict’s solution
Place test tube into a warm water bath for 10 minutes
Brick red if starch is present and sugars may be present but the colour possibly depend on the concentration of the concentration of the sugar
Record all observations in a results table

Question 26

Test for lipids

Answer 26

Place a small amount of food sample into a test tube
add a few drops of ethanol to the test tube
shake the test tube and leave for 1 minute
pour the solution into a test tube containing water
milky white layer should be produced from reaction

Question 27

Test for protein

Answer 27

Place a small amount of food sample in a test tube
Add 1cm3 of biuret reagent
Biuret reagent turns to purple

Question 27

Test for protein

Answer 27

Place a small amount of food sample in a test tube
Add 1cm3 of biuret reagent
Biuret reagent turns to purple