organication Flashcards
A
patient has a disease. The disease damages his pancreas.
A
doctor prescribes a course of treatment for the patient:
‘Take
one capsule with each meal.’
Each capsule contains hundreds of small, dry
beads.
The beads are made of enzymes. The pancreas normally produces these enzymes.
The outer coating of the capsule is made of lipid.
1
(a)
One enzyme in the beads is lipase.
In
a healthy person, lipase is made in the pancreas.
Name
two other enzymes made in the pancreas of a healthy person.
1.
2.
(2)
protease
amylase
The lipid coating on the capsule makes sure that the enzymes are not released until the
capsule reaches the small intestine.
Explain
how.
Lipase (that breaks down the fat) in the small intestine.There is no lipase in the stomach as it is not a good environment for lipase
The lipase in the beads does not digest the lipid coating around the capsule.
Suggest
why.
enzymes only work at the presence of water
Explain why less light passes through the mixture when the starch is more concentrated.
mixture becomes dark
The manufacturer adds carbohydrase from each of three different microorganisms, A B
and
C , to starch in flasks at 40 40°C.
Every minute
a sample of the mixture is added to iodine solution and placed in the
colorimeter.
Graph
2 shows these results.
(i)
When the concentration of starch reaches 2 %, digestion is considered to be
sufficient for the next stage in the manufacture of the slimming food.
How long does this take for the most effective carbohydrase?
Show clearly how you work out your answer.
minutes
(2)
around 7 minutes
Explain why the manufacturer of the enzyme carried out the investigation at
40°C.
at this temperature enzymes work best
Name the enzyme which would be used to convert glucose into fructose.
isomarase
Explain why fructose, rather than glucose, is used in slimming
foods.
Fructose is sweeter that sugar so less is needed to make the product sweet
What name is given to an enzyme which catalyses the breakdown of protein?
protease
What product is formed when protein is broken down by the
enzyme?
(
amino acid
pH
1.0
2.0
3.0
4.0
5.0
Rate
of formation of product
in mmol per minute
10.5
23.0
10.5
2.5
0.0
The enzyme is produced by the human digestive system.
(i)
At what pH does this enzyme work best?
(1)
2
Suggest which part of the digestive system produces this
enzyme that works the bast at 2ph.
(1)
stomach
Why is it necessary to break down proteins in the digestive system?
proteins are too large to be absorbed by gut
Name the type of enzyme that digests stains containing fats.
Lipase
A new detergent is marketed as being ‘environmentally friendly’.
Scientists compared the performance of this new detergent with an existing
detergent.
They measured the time taken by the two detergents to remove
a fat stain at different
temperatures.
The graph shows their
results.
Describe the effect of increasing the temperature on the time taken by the existing
detergent to remove the stain.
the best temperature for the enzyme is around 40.
at first as themerature increases the ability to remove the stains increases, but then when the temerature is too high, the ability to remove stains decreases.
The new detergent works at
a lower temperature than the existing one.
Is
the new detergent likely to be more ‘environmentally friendly’ than the existing
detergent?
Draw
a ring around your answer. Yes No
Explain the reason for your
answer.
(
yes, the lastt heat is needed so less energy is used; therefore, less polution.
Neither detergent works well at 60 60°C.
Explain
why.
enzymes are destroyed
A
group of students investigated the effect of temperature on the action of the enzyme lipase.
The
students:
•
put 1 cm 3 of lipase solution into a test tube
•
put 5 cm 3 of lipid into a different test tube
•
put both tubes in a water bath at 5 °C for 3 minutes
•
mixed the lipid with the lipase solution.
5
Every five minutes the students tested
a sample of the mixture for lipid, until no lipid remained.
The students repeated the experiment at different temperatures.
(a)
To make their investigation fair the students needed to control some variables.
Give
one variable the students controlled in their investigation.
(1)
same volume of lipase solution
The tubes of lipase solution and lipid were kept separately in the water bath for 3 minutes
before mixing. Why?
So that the lipase and the lipid reached the right
temperature
Describe the effect on the breakdown of the lipid of increasing the temperature from 5 °C to
5050°C.
(
Temperature in °C
Time taken until no lipid remained in minutes
5 40
20 15
35 5
50 30
95 lipid still there after 120 minutes
according to the table the best temperature is 35. as the temperature increasesover 35, more lipids remain
Suggest two ways in which the students could have improved their investigation.
Use information from
the students’ method and the results table to help you.
1: repeat the investigation
2. compare the result with others
The lipase did not break down the lipid at 95°C.
Why?
lisase is destroyed at such temperature