Organic components of saliva Flashcards
What is the average range of the total amount of protein found in saliva?
Approx 1-6 g/L
What factors affect the total salivary protein content?
Total salivary protein content varies according to flow rate and relative contribution from different salivary glands
What are the salivary proteins broadly categorised into?
They are categorised according to function:
- Proteins with lubricating properties
- Proteins with anti-microbial properties
- Enzymes
- Proteins with mineral binding properties.
What are the 7 main salivary proteins?
- Salivary mucins
- Statherin
- Proline rich proteins
- Histatins
- Immunoglobins (antibodies)
- Enzymes
- Lactoferrin
What are salivary mucins?
Salivary mucins are glycosylated proteins containing more than 40% (sometimes as much as 80%) carbohydrate on a weight basis.
What are salivary mucins important for?
- Mucins are important lubricants in the mouth
2. They protect the hard and soft tissue surfaces from damage
How many of the salivary proteins are salivary mucins?
25%
How and why are salivary mucins good lubricant?
The lubricant properties of these salivary glycoproteins is directly related to their structure
What is the structure of salivary mucins?
Carbohydrates are added to proteins by covalent attachment to the amino acid side chains. Two types of attachment are found, O- and N- linkages
his occurs after the polypeptide chain has been synthesised, via specialised enzymes in the endoplasmic reticulum and Golgi and is an example of protein post-translational modification.
Which sugar linkage is more abundant in salivary mucins: O or N linked mucins?
O type linkage is far more abundant
What are O- links sugars added to?
To serine (SER) or threonine (THR) amino acid residues within the mucin polypeptide chain via their side chains, which both terminate with an hydroxyl group. This forms a O-linkage result
What are N- links sugars added to?
N-linked sugars are attached to the amide nitrogen of the side chain of asparagine (ASN) residues within the polypeptide chain
Where are carbohydrates added?
Carbohydrate is only added to asparagine side chains where the asparagine occurs as part of the sequence asparagine-other amino acid-threonine or serine.
What do we call a sequence which tells the enzymes to add a modification to the amino acid?
A consensus sequence
What are carbohydrates attached via N- type linkages usually?
Oligosaccharides
What do oligosaccharides contain?
more than one carbohydrate residue
What is the one thing all oligosaccharides have in common?
They all share a common core structure, based upon N-linked N-acetylglucosamine where further sugars are then attached to
They may have a different number and types of carbohydrates attached
What are some examples of some oligosaccharides?
Sialic acid (N-acetylneuraminate) is frequently part of the oligosaccharide structure in salivary mucins
What does the presence of substituted carbohydrates like sialic avid confer?
The presence of substituted carbohydrates such as sialic acid confers a negative charge on salivary mucins and results in an extended structure for the proteins in solution due to repulsion of charges.
What do mucins compromise of?
Carboxyl- and amino terminal domains that are rich in cysteine (CYS) residues which are capable of forming covalent cross links through their sulphydryl (-SH) side chains
In this way, more than one of these monomeric structures can be linked together, in a linear fashion, to form mucin oligomers.
What are oligomers?
More than one mucin monomer linked together?
What is the large central portion of the salivary mucin MG1 made of?
The large central portion of the molecule is formed of multiple repeats of 10 to 80 amino acid residue sequences in which up to half of the amino acids are serine or threonine. This area becomes saturated with hundreds of O-linked oligosaccharides.
What are the 2 types of salivary mucins called?
MG1
MG2
Describe the MG1 salivary mucin
Is a high molecular weighted mucin
Is a picture of 3 different gene products
Is an oligomeric structure with a molecular weight greater than 1MDa
MG1 form complexes with other salivary components including amylases, proline-rich proteins, statherin and histamines and human defence peptides
Describe the MG2 salivary mucin
Derived from a single gene
Is a single monomer with molecular weight of around 250kDa
MG2 bind to oral pathogen and genes like oral Streptococci and there periodontal pathogen (Actinobacillus actinomycetemcomitans)
What is similar about both MG1 and MG2?
They are both produced in different amounts by the major and minor salivary gland
What does the MG in MG1 and MG2 stand for?
MG stands for mucous glycoprotein)
What are the 6 functions of salivary mucins?
- Protections for oral surfaces against Dissection
- Lubrication of hard and soft tissues to protect from mechanical damage and to facilitate in speech and swallowing
- Provide a barrier to microbial colonisation (In addition, purified MG2 has been shown to kill oral bacteria as well as the opportunistic yeast,)
- Role in regulation of other salivary proteins and peptides
- Anti microbial and anti fungal activity
- Direction of bacterial colonisation.
What are the functions of Statherin?
- Protects against ectopic calcification within the oral cavity by inhibiting the spontaneous precipitation of hydroxyapatite mineral.
tatherin binds to hydroxyapatite mineral and inhibits its growth. In addition, it binds calcium and so prevents the spontaneous precipitation of hydroxyapatite, reducing the possibility for calculus formation.
Statherin is an important component of the salivary pellicle – the thin protein-rich deposit which cover the surface of the teeth.
What can happen if there are too many mineral ions in saliva?
high concentration of mineral ions in saliva could lead to unwanted deposition of mineral if uncontrolled.
Calculus (calcified plaque or tartar) can build up leading to periodontal (“gum”) disease and stones can block salivary gland ducts
What is the nature of the primary structure of Statherin?
It has an asymmetric nature
Describe the primary structure of statherin
The amino terminal of the molecule is highly charged, with five consecutive amino acids with side chains that are negatively charged at physiological pH
Which section of the primary structure of statherin binds to calcium and why?
Amino acid residues 1-6 bind calcium tightly and so prevent spontaneous precipitation of hydroxyapatite from saliva.
Describe the secondary structure of statherin
Statherin has an unusual secondary structure because it contains a relatively high proportion of the imino acid PROLINE (PRO) precluding the alpha helix structures found in many other proteins.
What does the whole statherin molecule bind to in saliva and what does this binding do?
The whole of the Statherin molecule binds to existing hydroxyapatite mineral and inhibits further crystal growth
What role do proline rich proteins (PRPs) have in saliva?
They control mineral crystal growth and are important components of the salivary pellicle
What is the salivary pellicle?
The thin protein-rich deposit which covers the surface of the teeth.
What proportion do Protein rich proteins make up of the total salivary protein
PRPs make up 10-40% of the total salivary protein
Where is there a rich supply of proline rich proteins?
The sub mandibular gland
Other than in saliva where else are PRP found?
PRPs are also found in the secretions of the trachea and pharynx
Where are proline rich proteins derived from?
Proline rich proteins are polymorphic and derive from a group of genes situated on chromosome 12
How many proline rich proteins have been identified?
Six different PRPs have been identified so far, all closely related in respect of their primary structure.
What is the average concentration of proline rich proteins in saliva?
approx 50 mg/100mL