Organelles of the Eukaryotic Cells Flashcards

1
Q

What proportion of a eukaryotic cell’s volume is occupied by organelles

A

Organelles occupy about half of the volume of a eukaryotic cell.

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2
Q

What are the main components of the eukaryotic cytoplasm

A

The cytoplasm includes the cytosol and cytoplasmic organelles suspended within it.

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3
Q

What is the function of mitochondria

A

Mitochondria are involved in energy metabolism and other metabolic functions.

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4
Q

What are the functions of the endoplasmic reticulum (ER)

A

The ER synthesizes specific proteins for membranes or secretion, modifies proteins, and synthesizes lipids.

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5
Q

What is the function of peroxisomes

A

Peroxisomes are sites of oxidative metabolism.

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6
Q

What is the role of endosomes in the cell

A

Endosomes process endocytosed particles on their way to lysosomes or vacuoles.

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7
Q

What is the function of lysosomes and vacuoles

A

They contain digestive enzymes to degrade endocytosed particles, old organelles, and macromolecules.

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8
Q

Why do eukaryotic cells need internal membranes

A

Because large cells have a low surface area to volume ratio, internal membranes expand membrane surface for energy production and specialization.

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9
Q

What are the advantages of subcellular compartmentalisation in eukaryotes

A

It allows metabolic specialisation and reduces competition between intermediates.

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10
Q

What does it mean when organelles are topologically equivalent

A

Molecules can travel between compartments without crossing a membrane or entering the cytosol.

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11
Q

How do mitochondria and chloroplasts differ from other organelles

A

They have a double membrane, an endosymbiotic origin, and are isolated from vesicular trafficking.

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12
Q

What forms the junctions between cristae and the intermembrane space in mitochondria

A

Multiprotein complexes MICOS and OPA1.

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13
Q

What creates the curvature of mitochondrial cristae

A

The angle between ATP synthase dimers at the tips of cristae.

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14
Q

What is the ‘respirasome’

A

A supercomplex formed by all ETC components on the side of cristae to funnel protons to ATP synthase.

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15
Q

How do cristae act as insulated transformers of metabolic energy

A

Narrow necks and dense ETC protein packing limit proton diffusion and leakage.

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16
Q

What causes thylakoid stacking in chloroplasts

A

PSII-LHCII complexes which increase surface area and light capture.

17
Q

What are etioplasts and gerontoplasts

A

Etioplasts are chloroplast progenitors; gerontoplasts are aging chloroplasts.

18
Q

What are chromoplasts and desiccoplasts

A

Chromoplasts store carotenoid pigments; desiccoplasts exist in desiccation-tolerant plants.

19
Q

What do elaioplasts, proteinoplasts, and amyloplasts store

A

Lipids, proteins, and starch, respectively.

20
Q

How many genes are in the human mitochondrial genome

A

37 genes: 13 for ETC subunits, 22 tRNAs, and 2 rRNAs.

21
Q

How many genes are in the Arabidopsis chloroplast genome

A

117 genes: 87 for proteins, 8 for rRNAs, and 37 for tRNAs.

22
Q

Why can mitochondria and chloroplasts not function independently despite having DNA

A

Most required proteins are encoded in the nucleus and imported from the cytoplasm.

23
Q

How are proteins imported into mitochondria

A

They must be unfolded by chaperones to pass through the translocase pore.

24
Q

What are the four chloroplast protein import pathways

A

Sec pathway, SRP-like pathway, TAT pathway, and spontaneous insertion pathway.

25
What does the TAT pathway rely on for chloroplast protein import
Twin arginine residues in the signal peptide.
26
What initiates mitochondrial fission
Dynamin-1 GTPase forms spirals that constrict and sever the outer membrane.
27
How does mitochondrial outer membrane fusion occur
Via outer membrane GTPases forming tethering complexes between two membranes.
28
What mediates inner membrane fusion in mitochondria
A dynamin-related GTPase forms oligomers that tether and fuse the inner membranes.
29
What is the main metabolic function of peroxisomes
Use oxygen to remove hydrogen from substrates, producing hydrogen peroxide.
30
How do peroxisomes appear under electron microscopy
They have a dense crystalloid protein core.
31
How do peroxisomes change based on yeast growth conditions
On sugars: small/few peroxisomes; on methanol or fatty acids: large/numerous peroxisomes.
32
Where does beta-oxidation of fatty acids occur in mammals vs. yeast/plants
In both mitochondria and peroxisomes in mammals; only in peroxisomes in yeast/plants.
33
What is a role of peroxisomes in photosynthesis
Detoxifying byproducts when Rubisco binds O₂ instead of CO₂.
34
How are peroxisomes formed and maintained
By fission using dynamin proteins and fusion of ER-derived precursor vesicles.
35
Do peroxisomes have their own DNA
No, they lack genomes and rely on protein import from the cytoplasm and ER.
36
Where does β-oxidation of fatty acids take place in plants
In peroxisomes
37
Where does β-oxidation of fatty acids take place in animals
In both mitochondria and peroxisomes
38
What is produced during the first oxidation step in peroxisomal β-oxidation
Hydrogen peroxide (H₂O₂)
39
What does catalase do during peroxisomal β-oxidation
Breaks down hydrogen peroxide into water and oxygen