Organelles

Question 1

What are mitochondrial proteins synthesised with?

Answer 1

N-terminal pre sequence

Question 2

How is mitochondria import studied?

Answer 2

In vivo - use of yeast
Many mutations that affect mitochondrial protein import generate respiration deficiency mutants, which will only grow on fermentation conditions

Question 3

What are the characteristics of the matrix targeting signals?

Answer 3

Amphipathic (produces a polar and a hydrophobic side) and often helical
No specific sequence - can build from only three amino acids presuming the encoded peptide has an amphipathic character

Question 4

What are the initial receptors for TOM?

Answer 4

Tom20 and tom70

Question 5

What are the core TOM components?

Answer 5

TOM40

Question 6

What does Tom stand for?

Answer 6

Translocase or the mitochondrial outer membrane

Question 7

What are the two translocase components of the mitochondria called?

Answer 7

TOM and SAM