Order Of Draw Flashcards
- Yellow
Additive: SPS
Sample: whole blood
Lab: microbiology
Inversions: 8-10
*maintain sterility
*aerobic cultures then anaerobic
- Light Blue
Additive: sodium citrate
Sample: plasma
Lab: coagulation
Inversions: 3-4
*Sample tube must be filled to indicator line for valid test results; may use throwaway tube
*common test: protime, PTT, fibrinogen, coagulation studies
- Gold
Additive: SST (serum separator)
Sample: serum
Lab: chemistry
Inversions: 5
- Invert tube for clot activation, allow to clot for 30 minutes
*Common test: lipid profile, thyroid profiles, hepatitis profiles, HIV profiles
- Tiger
Additive: SST
Sample: serum
Lab: chemistry
Inversions: 5
- Red
Additive: SST/none
Sample: serum
Lab: chemistry
Inversions: 5
*Common test: metabolic blood panel
*use for serum collections when gel barrier tubes should not be used
- Dark green
Additive: lithium heparin
Sample: Plasma
Lab: chemistry
Inversions: 8-10
- Light green
Additive: sodium heparin
Sample: Plasma/serum
Lab: hematology
Inversions: 8-10
- Lavender
Additive: EDTA
Sample: Plasma
Lab: hematology
Inversions: 8-10
*Common tests: CBC, ESR, hemoglobin A1C
*do not send clotted specimens
- Pink
Additive: EDTA
Sample: plasma
Lab: blood bank
Inversions: 8-10
*common tests: blood typing
- Grey
Additives: potassium oxalate, sodium fluoride
Sample: plasma
Lab: chemistry
Inversions: 8-10
*common test: glucose testing, lactic acid testing
- Yellow (ACD)
Additive: ACD (acid citrate dextrose)
Sample: Plasma
Lab: chemistry
Inversions: 8-10
*solution A and B are interchangable
- Royal Blue
draw the royal blue tube tops in the order indicated by the additive
- Royal Blue with RED strip = no additive. Draw after the red tube or SST tubes. Used to measure trace metal within serum.
- Royal Blue with GREEN strip = sodium heparin. Draw after heparin tubes.
- Royal Blue with LAVENDER strip = EDTA. Used to measure trace metal within whole blood.
Additive: EDTA/none
Sample: plasma/serum
Lab: toxicology
Inversions: 8-10
What are the SST tubes?
Gold, Tiger, Red
What are the heparin tubes?
Dark and light green
What are the EDTA tubes?
Lavender, Pink, and Royal Blue with Lavender strip
Describe the order of draw by additive
SPS/culture medium - keep sterile
Sodium Citrate
SST
Lithium Heparin/Sodium Heparin
EDTA
Potassium Oxalate/Sodium Fluoride
ACD
What does the SPS additive do and in what tube?
SPS= sodium polyanthole sulfonate
It is a common anticoagulant and inhibits the activity of the antimicrobial substances in blood, which allows microbes to grow.
The first 2 tubes: aerobic and then anaerobic.
Which tube is sodium citrate in and what does it do?
Acts as an anticoagulant so the sample remains stabe for testing in the light blue tube.
What tubes are SST in and what does it do?
A serum seperator tube which causes blood clotting, and it creates a barrier between serum and blood cells. This allows for easy serum collection after the centrifuge.
Gold, tiger, and red.
What does heparin do and what tubes is it in?
Dark green tube has lithium heparin, and light green has sodium heparin. These are plasma seperation tubes and blood clotting is inhibited/delayed.
Sometimes it goes on ice.
What tubes have EDTA and what does it do?
EDTA prevents blood from clotting by removing calcium (that aids in clotting). used for complete blood counts.
In lavender and pink tubes.
What does potassium oxalate/sodium fluoride do and in what tube is it in?
The grey tube. It is used to preserve the blood sample. Sodium fluoride prevents glycolysis which preserves glucose for glucose tessting.
What tube is ACD in and what does it do?
ACD prevents clotting and is used for hematology or genetic testing. (ABO or Rh typing). The yellow tube.
