Oral Biology L2 - Biochemistry and molecular biology context

Question 1

what is biochemistry?

Answer 1

the study of chemical processes relating to living organisms. helps us understand how organisms grow and replicate, and so can help us understand problems with growth, and disease processes.
- is the bridge between physical and biological sciences

Question 2

what is the central dogma of molecular biology?

Answer 2

the flow of genetic information within a biological system. It is often states as ‘DNA makes RNA and RNA makes protein.’
- not necessarily true for viruses

Information -> messenger RNA -> proteins with functions

Question 3

what are the important experimental techniques used to study genes, gene expression and proteins?

Answer 3

• PCR
• DNA sequencing
• gene editing: CRISPR-CAS

Question 4

what are the important experimental techniques used to study gene expression?

Answer 4

• northern blotting
• qRT-PCR
• RNS-Seq

Question 5

what are the important experimental techniques used to study protein expression?

Answer 5

• SDS polyacrylamide gel electrophoreses
• mass spectrometry

Question 6

what are the important experimental techniques used to study protein structure?

Answer 6

• X-ray crystallography
• cryo-electron microscopy