Optical Components of the Eye Flashcards

1
Q

Describe 3 general constraints on eye design

A

Limited by available radiation
Availability of detection mechanisms (photopigments)
Animal dimensions (eye size/position, brain)

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2
Q

Could we use IR, UV or Radiowave radiation instead of visible light?

A

IR: Thermal body energy would imapir our VA
UV: damages retinal ph-receptors, most absorbed by cornea (too much causes photokeratitis)
Radio: pupil diameter would need to be 400+m for desired resolution

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3
Q

Describe the difference between an eyespot and eyepit design

A

Eyespot: single, small, flat spot of photosensitive pigments provides very crude light detection/direction sensitivity
Eyepit: light-sensitive cells arranged in depression for better directional sense but poor resolving power

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4
Q

Describe a pinhole eye and it’s pros/cons

A

inverted image requiring extra neural processing (e.g. Nautilus Mollusca)
simple designs form single image with much better direction discrimination
Diffraction limits resolving power, limited FoV, small, fixed size results in faint image (poor scotopic vision)

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5
Q

Describe the vesicular eye

A

epithelial surface (cuticle) grows over pinhole protecting against FBs, also has lens of irregular shape/n and irregular fluid-filled chambers

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6
Q

Describe the lens eye and its pros/cons

A

cornea (evolved from cuticle), small, regular lens, humours, multiple photosensitive cells arranged into cup, adaptable pupil (iris)

Light hits single point on retina only for great directional sensitivity, bigger/adaptable aperture (pupil) adjusts focussing power for sharper/brighter image

very complex image at photoreceptors requires greater neural processing/time to extract relevant info

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7
Q

Describe apposition compound eyes and the pros/cons

A

common in diurnals, ommatidia provides multi-feed for motion detection, upright image forms requiring less neural processing/scanning
Con: each cone lens feeds 1 rhabdom limiting eye sensitivity so more suited for diurnal

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8
Q

Describe the structure of an ommatidium

A

cornea, gradiented cone-shaped lens, Rhabdom (tight-packed transparent rods enhances photon absorption) diverts light outwards to retinular cells (rhodospin photopigments absorb light ~ transmit signals along nerve fibres to CNS

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9
Q

Explain the benefit of a gradiented-index cone lens

A

relative increase in angle of acceptance of light

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10
Q

Explain superposition eye design

A

multiple cone-lens array collects/redirects light towards a given rhabdom (array) improving sensitivity
2 piece gradiented cone-lens length is twice as big as apposition lens allowing light to be re-collimated towards single rhabdom

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11
Q

Compare superposition/apposition eye differences

A

Superposition show larger angle of acceptance (multiple longer cone-lenses provide enhanced sensitivity ideal for nocturnal)
Also have better motion detection

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12
Q

Describe the pre-corneal tear film structure/function

A

lipid (meibomian), aq (lacrimal), mucus (conjunctival goblet cells) ~ 8um

smooth refracting surface assists cornea optics, lubricates lids/cornea lowering friction, anti-microbial, removes debris, regulates external hydration/O2 supply to cornea

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13
Q

Explain the optical role of the tear film

A

ignore presence in calculations
varying thickness can induce aberrations (unwanted distortions) e.g. crying blur, cold winds, staring

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14
Q

Describe the corneal stroma ultrastructure

A

individual parallel collagen fibrils arranged in quasi-regular lamellae (200-250, 12mm long) perpendicular to each other (vital for transparency)/extracellular matrix

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15
Q

Explain Corneal Transparency

A

stroma acts as a complex 3D grating with ~50nm inter-fibril-spacing: forward incident difracted waves cause constructive interference, obliquely diffracted waves cause destructive

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16
Q

Why are scleral tissues comparatively opaque to the stroma despite both being formed of collagen fibrils?

A

outer sclera: variable fibril diameter (70-300nm)
middle/inner: irregular inter-fibril spacing
overall scleral tissue can’t act as 3D diffraction grating so signficant light scattered (milky white appearance)

17
Q

Describe the aspheric corneal contour using the conicoid approximation

A

aspheric design reduces total spherical aberration for higher quality retinal image

anterior surface approximated to ‘elliptical conicoid’ describes central 8mm

harder to distinguish p values for central 3mm

18
Q

How does ACD change with age & accommodation? Why is this a crucial pre-op measure?

A

ACD decreases so required accommodation increases
big influence on IOL power selection for implants/phacoemulsification

19
Q

Describe pupil function and its adaptation

A

reactive, controls light, diameter influenced by age/refractive error
size affects aberrations/diffraction/depth of field
diameter changes alone can’t maintain retinal sensitivity to adapt for change from photopic to scotopic so specialised neural/retinal adaptations compensate for this

20
Q

What is the significance of the aspheric curvature of the lens’ surfaces?

A

degree id peripheral flattening reduced total spherical aberrations for high-qualiy retinal image

21
Q

Describe the crystalline lens structure

A

fibrous, light-scattering bundle of fibres with varying n boundaries, transparency reduces with age due to brunescence (less blue light transmitted)

continous gradiented n (due to packed lens fibres in cortex)

22
Q

Describe the Vitreous Body

A

stagnant humour with hyaloid remnants (mainly water, becomes less viscous with age so risk of macula hole) perfectly transparent in youth

23
Q

Explain how the eye sub-structures absorb EM radiation

A

cornea absorbs <320nm
lens absorbs blue light better with age due to lenticular brunescence protecting foveal cone pigments
liquid humours absorb IR at specific wavelength absorption bands
macula pigments (LZ) absorb high energy blue light protecting RPE/foveal cones

24
Q

Describe 3 uses of retinal reflectance

A

most reflected light in red to near-IR region allows: retinoscopy reflex, non-mydriatic fundus photos, autorefractors

25
Describe the Stiles & Crawford Experiment
perpendicular beams appear brighter than peripheral entering beams variable density filter adjusts density to reduce axial beam intensity till both halves appear equally dim results show foveal cones specially adapted to capture light specifically through pupil centre compared with rods
26
Explain 2 theories for the SCE effect
Photoreceptor positioning: foveal cones develop pointing towards pupil centre to capture brightest light Retinal optics: cones show narrower acceptance angle than rods, causing TIR in photoreceptor outer segments
27
What's the benefit of the SCE?
reduced oblique, scattered light to foveal cones so better retinal image contrast/visual perfomance as light not trapped by foveal cones
28
What assumptions are made for Purkinje Image formations?
only truly holds for incident angles <10degs so reflected rays show negligible polarisation only truly holds for smooth surfaces, 2 media with well-defined n assume lens has homogenous refractive index
29
How do Purkinje images and lens curvature change with accommodation?
PIII forms forward within lens towards the cornea PIV forms backwards within lens towards retina Ant. lens shows more steepening than post.
30
Where are the nodal points of a real relaxed human eye?
straddling the posterior crystalline lens surface
31
Define angle lambda
formed between pupillary axis and line-of-sight at entrance pupil (E) and then exit (E') to fovea (F) ~ easy to measure clinically under monocular viewing (Kappa test)
32
Define angle kappa
formed between pupillary axis and visual axes at first nodal point N and then N' to fovea (F) ~ easy to clinically measure under binocular viewing (Hirschberg test)
33
Define Nyquist's theorem
max spatial frequency that can be detected/resolved = 1/2 the foveal ph/receptor sampling frequency i.e. 2 cones resolve a full cycle (1 cone maximal, 1 minimal signal) adult cone spacing ~ 120/deg so max theoretical resolvable spat. f = 60 cycles/deg (6/3 snellen)