Operating Instruments Flashcards
1
Q
What are the steps for operating a pH meter?
A
- Ensure the pH meter is clean and calibrated.
- Turn on the pH meter using the power button.
- Rinse the electrode with distilled water
- Immerse the electrode into the solution you wish to test.
- Allow the reading to stabilize, then record the pH value displayed.
- Rinse the electrode again before storing the pH meter.
2
Q
What are the steps for operating a UV-VIS spectrophotometer?
A
- Ensure the instrument is plugged in and turned on.
- Allow the machine to warm up for a few minutes.
- Set the wavelength on the display according to your experimental needs.
- Insert a blank cuvette filled with the solvent into the sample holder and press ‘Zero’ or ‘Blank’.
- Remove the blank and insert the cuvette containing your sample.
- Press ‘Read’ to obtain the absorbance value.
- Log the results and clean the cuvette after use.
3
Q
What are the steps for operating an FTIR spectrometer?
A
- Ensure the instrument is correctly set up and connected to a power source.
- Turn on the FTIR spectrometer using the main power switch.
- Allow the instrument to perform a self-check or calibration.
- Prepare your sample and place it on the sample holder.
- Select the appropriate settings for your analysis (e.g., scan number, resolution).
- Press ‘Run’ to initiate the scan.
- Once the scan is complete, analyze the resulting spectrum displayed on the screen.
- Save the data for further analysis.
4
Q
What are the steps for operating a fluorescence spectrometer?
A
- Check that the spectrometer is turned off before connecting the power supply.
- Turn on the instrument and allow it to warm up.
- Set the excitation and emission wavelengths according to your sample requirements.
- Prepare your sample in a suitable cuvette.
- Insert the cuvette into the sample compartment.
- Press ‘Start’ or ‘Measure’ to begin the fluorescence measurement.
- Record the fluorescence intensity and any other relevant data.
- Remove the cuvette and clean it before storage.
