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Paediatrics RACP written exam > Oncology/Haematology > Flashcards

Oncology/Haematology Flashcards

1
Q

Poor prognostic signs in ALL

A

WCC>50
<2 and >9 years age
Boys do less well than girls
Chromosomal abnormalities eg t(4:11) and t(9:22)
Hypodiploidy <44
CNS disease
Poor response to induction chemo with minimal residual disease

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2
Q

Ifosfamide side effects

A

Metabolic acidosis

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3
Q

Wilm’s tumour is associated with which conditions?

A
  • WAGR syndrome 30%, WT1 deletion- Wilms tumour; aniridia; genitourinary abnormalities; retardation
  • Denys-Drash syndrome 90%, WT1 missense mutation - pseudohermaphroditism; mesangial renal sclerosis, Wilms tumour.
  • Fanconi Anaemia (20%)
  • Beckwith-Wiedemann syndrome 5%, 11p15
    Hemihypertrophy
    Cryptorchidism
    Hypospadias
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4
Q

Risk factors for development of sarcoma?

A
  • Li-Fraumeni syndrome (p53 mutation)
  • NF1
  • Hereditary retinoblastoma (500x risk osteosarcoma)
  • Prior treatment with radio/chemotherapy (inc osteosarcoma risk)
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5
Q

Side effects of methotrexate?

A

Mucositis
Hepatitis
Decr renal function
Neurocognitive effects

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6
Q

Side effects of cisplatin?

A

Ototoxicity (can use sodium thiosulfate to decrease hearing loss risk)
Nephrotoxicity
Delayed nausea

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7
Q

Side effects of ifosfamide and cyclophosphamide?

A

Haemorrhagic cystitis
Leukaemia/lymphoma
Infertility
Lung fibrosis

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8
Q

Side effects of vincristine?

A 
Peripheral neuropathy
Abdominal pain, constipation
Jaw and bone pain
Ptosis
Extravasation injury
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9
Q

Side effects of anthracyclines/doxorubicin?

A

AML
Cardiomyopathy
Radiation-recall dermatitis
Necrosis on extravasation

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10
Q

Side effects of etoposide?

A

Leukaemia (AML)

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11
Q

Side effects of L-asparaginase?

A

Hyperglycaemia
Pancreatitis
Coagulopathy
Encephalopathy

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12
Q

Side effects of bleomycin?

A

Pulmonary fibrosis

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13
Q

Chemotherapy drugs causing an increased risk of secondary malignancy?

A
  • Cyclophosphamide (alkalising agent)
  • Etoposide (topoisomerase II inhibitor)
  • Doxorubicin and danorubicin (anthracyclines, topoisomerase II inhibitors)
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14
Q

Side effects of radiotherapy?

A

Neurocognitive
Endocrine: growth hormone deficiency, hypotyhroidism
Malignancy: breast, thyroid, sarcoma, lymphoma
MSK atrophy
Organ damage: cardiac, lung, GI

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15
Q

Describe the types of Non-Hodgkin’s lymphoma

A
  • Burkitt’s lymphoma with majority of B cells
  • Lymphoblastic lymphoma with 80% T cells and 20% B cells
  • Diffuse large B cell lymphoma with B cells
  • Anaplastic large cell lymphoma with 70% T cells, 20% null cells and 10% B cells
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16
Q

Describe veno-occlusive disease (VOD) or sinusoidal obstruction syndrome (SOS)

A
  • Obstruction of small veins in the liver as a complication of high-dose myeloablative chemotherapy given before a bone marrow transplant
  • Due to injury to hepatic venule, dilation of sinusoids and hepatocyte necrosis, sclerosis, collagen deposition -> obliteration and necrosis, fibrous tissue replacement of normal liver
  • Triad of: weight gain, painful RUQ hepatomegaly, jaundice
  • Onset within 30 days of SCT, occurs in 10-60%
  • Ascites and hyperbilirubinaemia
  • Image with doppler USS or CT
  • Can treat with defibrotide (anti-thrombus, anti-inflamm). High mortality rates when associated with multiorgan failure
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17
Q

Target cells and tear drop cells are consistent with?

A

Thalassaemia trait

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18
Q

What is haemoglobin made up of and how is it made?

A 
4 globin chains + haem
Fetal = a2 + y2
Adult = a2 + b2
Haem is developed in the mitochondria of developing erythroblasts. Haem = porphyrin (from Vit B6) + iron.
90% of EPO comes from kidney.
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19
Q

Which factors shift the oxygen dissociation curve to the left?

A

Shift to left = increased affinity for oxygen

Alkalosis, low CO2, low temp, low 2,3 DPG, HbF

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20
Q

Which factors shift the oxygen dissociation curve to the right?

A

Shift to right = decreased affinity for oxygen
Acidosis, hight CO2, high temp, high 2,3 DPG, Hb S, exercise
i.e. shifts to right when tissues need more oxygen
“Right Raised Reduced affinity”

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21
Q

What is Hb Barts?

A

4 x fetal gamma chains due to 4 x alpha deletions

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22
Q

Describe the types and consequences of alpha thalassaemia

A

1 alpha missing - silent carrier, normal FBC
2 missing - alpha thalassaemia trait: mild anaemia
3 missing - HbH disease/alpha thalassaemia intermedia: mod anaemia, mild haemolysis, not transfusion dependant, HSM and skeletal changes
4 missing - Hb Barts: hydrops fetalis, newborn death due to severe anaemia, congestive heart failure. Can do IU transfusions and then BMT postnatally
BF: hypochromic, microcytic, target cells, golf-ball like
- Leads to excess b or y chains, abnormal O2 dissociation curves

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23
Q

Describe the types and consequences of beta thalassaemia

A
  • Autosomal recessive, B+ (partial function), Bo (no function)
  • Minor- B/Bo or B/B+. Mild microcytic anaemia, no Tx
  • Intermedia - B+/B+ or B+/Bo. Mod haemolysis, mod-severe anaemia, not transfusion dependant, splenomegaly
  • Major - Bo/Bo. Severe haemolysis from 3-6m life (after HbF decreases) transfusion-dependent, HSM, iron overload, bony deformities, “hair on end” skull x-ray, may need splenectomy
  • Inc HbA2% (>3.5% in minor) and HbF%, inc RBC distribution width
  • BF: microcytic hypochromic, target cells
  • High Fe, transferrin, ferritin
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24
Q

Management of thalassaemia

A

If Hb <60 for ?3/12 then need regular transfusions
Iron chelation therapy (deferiprone or deferasirox)
May requite BMT, splenectomy
Gene therapy in future

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25
Q

Describe the mechanism of sickle-cell disease

A
  • Single base mutation A->T which changes valine to glutamic acid on B-globin chain
  • HbS is insoluble, forms crystals when exposed to low O2 tensions.
  • Shifts O2 dissociation curve to right
  • Presents >6m age when HbF-> HbA
  • Sickle cell trait usually asymptomatic as long as well-oxygenated
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26
Q

Risks of sickle-cell disease

A
  • Crisis: vaso-occlusion with ischaemia-reperfusion injury
  • Haemolytic anaemia
  • Above precipitated by infection, acidosis, dehydration, deoxygenation
  • Infection risk: pneumococci, haemophilus, salmonella (due to impaired splenic function, defective complement activation, tissue ischaemia, micronutrient deficiencies)
  • Stroke - progressive vasculopathy, moyamoya-like syndrome, acute IH when older, cognitive defects due to silent brain infarcts
  • Aplastic crisis, often associated with parvovirus
  • Acute chest syndrome - pulmonary infiltrate, infection, embolism, vaso-occlusion
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27
Q

Management of sickle-cell disease

A
  • Penicillin prophylaxis + conjugate vaccines
  • Hydroxyurea - increases HbF concentration
  • RBC transfusions - decr HbS synthesis
  • BMT is curative (inc morbidity and mortality)
  • Opiates for acute pain in crisis
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28
Q

What are the causes of asplenia?

A

Surgical resection
Autosplenectomy (infarct e.g. sickle-cell)
Congenital asplenia syndromes
Iatrogenic/treatment for diseases (ITP, spherocytosis, thalassaemia)

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29
Q

What is hypersplenia?

A

High number of WCC, RCC, plts in spleen - get destroyed.
Due to infection (EBV, TB, hepatitis), alcohol, cirrhosis.
Splenomegaly.
Treat: underlying cause +/- splenectomy

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30
Q

Side effects of transfusion?

A

ABO-incompatibility - haemolysis, DIC, renal failure
Bacteria-contaminated infusion
Transfusion-related acute lung injury (TRALI) - ARDS-like picture
Fever, rigors - cytokine production
Anaphylaxis, IgE

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31
Q

Side effects of massive transfusion protocol

A 
Coagulopathy (relative decr platelets)
Volume overload
Hypothermia
Hypokalaemia
Hypocalcaemia
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32
Q

Describe transient erythroblastopenia of childhood

A

Pure red blood cell defect: low Hb, low retics, normal MCV
6m - 5y/o
Due to viral illness, spontaneous recovery
Expectant transfusion for symptomatic anaemia

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33
Q

Causes of anaemia

A
  • Decreased substrate (Fe, B12, folate)
  • Abnormal production (aplastic BM, malignancy, drugs, liver/renal disease, haemoglobinopathies)
  • Increased destruction (haemolysis, congenital, autoimmune, infection, hypersplenism)
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34
Q

Causes of aplastic anaemia

A
  • Constitutional: Fanconi anaemia, Dyskeratosis congenita, Schwachman-Diamond syndrome
  • Acquired: idiopathic, drugs (acetazolamide, chloramphenicol), infections (EBV, parvovirus, viral hepatitis), toxins (glues), paroxysmal nocturnal haemoglobinuria
  • Tx: HSCT or immunosuppression (cyclosporin)
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35
Q

Causes of iron deficiency anaemia

A 
Early weaning to cow's milk, cow's milk excess
Coeliac disease
Malabsorption
Chronic blood loss
Menstrual losses
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36
Q

What are the causes of microcytic anaemia?

A

Iron deficiency
Thalassaemia (beta or alpha)
Lead poisoning
Sideroblastic anaemia (iron present but cannot be utilised to make Hb)
Anaemia of chronic disease (infection, malignancy) - can be normocytic

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37
Q

Describe Fanconi Anaemia

A
  • AR, mean onset age 8y. Defect in DNA-repair, increased chromosomal breakage.
  • Marrow aplasia - all lines affected. Macrocytic anaemia
  • Microcephaly, absent thumbs + radii, cafe au-lait, short stature, cutaneous hyperpigmentation, decreased fertility (hypogonad), develop delay
  • Increased risk AML (30%) and solid tumours (25%)
  • Tx: androgens (inc RBC synthesis), steroids, HSCT
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38
Q

Describe Dyskeratosis Congenita

A
  • X-linked recessive, mean age 10 for skin, 17 for anaemia
  • Associated with short telomeres (repair regions), similar to premature ageing
  • Pancytopenia, hyperpigmentation, dystrophic nails, oral leukoplakia, pulmonary and liver fibrosis
  • Tx: androgens (inc RBC synthesis), splenectomy, HSCT
  • Inc risk oral, nasopharyngeal, vulvul cancers
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39
Q

Describe Shwachman-Diamond Syndrome

A
  • Neutropenia +/- anaemia + thrombocytopenia
  • Exocrine pancreatic insufficiency
  • Skeletal abnormalities (esp hip and knee)
  • Short stature
  • Can progress to marrow aplasia or AML
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40
Q

Diamond-Blackfan Syndrome

A
  • Newborn, 90% present <1yo. AR
  • Pure RBC aplasia, elevated HbF, inc RBC adenosine deaminase activity
  • Macrocytic, absent red cell precursors in marrow
  • Short stature, webbed neck, cleft lip, triphalangeal thumb
  • Late onset leukaemia
  • Tx: steroids (80% respond, 20% remit), transfusion, HSCT
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41
Q

Describe hereditary spherocytosis

A
  • Autosomal dominant
  • Blood film: spherocytes, polychromasia (inc immature RBC)
  • Bloods: DAT-ve, inc bili/retics/LDH, low Hb
  • Splenomegaly
  • EMA binding test (decr) + osmotic fragility test (inc fragility)
  • Tx: folic acid, splenectomy, cholecystectomy
  • Range from asymptomatic with mild anaemia to poor growth, jaundice, chronic transfusion requirement
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42
Q

Describe G6PD deficiency

A
  • X-linked, African/Mediterranean/SE Asian
  • Usually asymptomatic or prolonged jaundice
  • Acute haemolysis triggered by meds (aspirin, antimalarials, cotrimox, nitrofurantoin), infections, acidosis, fava beans
  • Decr NADPH (may be normal in acute haemolysis), low Hb, high retics
  • Tx: supportive (hydration, transfusion)
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43
Q

Heinz bodies + bite cells on blood film

A

G6PD (bite cells are where the Heinz body has been “bitten” out/phagocytosed by splenic macrophages)

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44
Q

Describe pyruvate kinase deficiency

A
  • Low PK level leads to low ATP which impairs RBC survival
  • Tolerate low Hb due to inc in 2,3DPG and shift O2 curve to R - increased O2 availability
  • AR
  • Haemolysis secondary to infections e.g. parvovirus, not aggravated by stress
  • Splenectomy to stop RBC destruction
  • Can get chronic haemolytic anaemia, unconjugated jaundice
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45
Q

Causes of DAT/Coombs+ve

A

ABO incompatibility/ haemolytic disease of newborn
AIHA
Drug-induced HA
Haemolytic transfusion reactions

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46
Q

Causes of polycythaemia

A
  • Primary (rare in childhood) - familial or congenital, polycythaemia vera
  • Secondary - heart disease (ToF, CHD), lung disease, high altitude, congenital methaemoglobinaemia, neonates, delayed cord clamping. All are a response to hypoxia with increased EPO production
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47
Q

Describe autoimmune haemolytic anaemia

A
  • Acute, self-limiting, often presents post virus (EBV, mycoplasma)
  • DAT/Coombs +ve, low Hb, high retics
  • Jaundice, splenomegaly, anaemia
  • Causes: infections, drugs (penicillin, quinidine), SLE/RA, lymphoprolif disease (Hodgkin’s)
  • Tx: supportive with folic acid, occ steroids, splenectomy, transfusions (but haemolyse), occ rituximab
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48
Q

Is beta thalassaemia associated with hydrops?

A

Not associated with fetal hydrops, as fetal haemoglobin (α2γ2) compensates

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49
Q

Most commonly implicated antigen in NAIT?

A

In Caucasian women it is Human Platelet Antigen 1a (HPA1a) which accounts for 85% of cases

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50
Q

Most commonly implicated antigen in NAIT?

A

In Caucasian women it is Human Platelet Antigen 1a (HPA1a) which accounts for 85% of cases. 3% population negative.

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51
Q

Abnormal thrombin time but normal reptilase time suggests?

A

Heparin (reptilase is resistant to inhibition by antithrombin III, thus is not prolonged in blood samples with heparin)

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52
Q

Causes of spherocytes on blood film?

A

Spherocytosis - reduced EMA binding

AIHA (usually warm HA)- normal EMA binding

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53
Q

What is Evan’s Syndrome?

A

Evans syndrome is an autoimmune haemolytic anaemia and thrombocytopenia. Coombs+ve.

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54
Q

Which factor stimulates eosinophil production?

A

IL-5

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55
Q

Causes of congenital neutropenia?

A
  • Kostmann syndrome (recurrent infection, ELANE or HAX1 mutation, cognitive defects and seizures)
  • Shwachman-Diamond syndrome (SS, pancreatic insuff, skeletal abnorm)
  • > significant infection history (pneumonia, abscess, gingivitis)
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56
Q

Describe chronic benign neutropenia

A

Autoimmune, can be post viral infection, at risk minor infections, usually ANA+ve, 95% remit in 6-24m. Can usually mount inc neutrophil response with infections (make neuts but destroyed by antibodies)

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57
Q

Causes of raised neutrophils

A 
Infection
Stress
Exercise
Seizures
Toxic agents 
Steroids
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58
Q

Causes of chronic neutrophilia

A 
Malignancy
JIA
Kawasaki
Burns
Uraemia
Post-op
Splenectomy
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59
Q

What cells do platelets develop from?

A

Megakaryocytes. Under control of thrombopoeitin and IL-6

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60
Q

Causes of thrombocytopenia?

A
  • Impaired production - thrombocytopenia + absent radius syndrome, Fanconi anaemia, Wiskott-Aldrich, aplastic anaemia
  • Decreased survival
    - Immune mediated: ITP, NAIT, SLE, drug-induced, malignancy
    - Non immune mediated: DIC, HUS, TTP, cyanotic CHD, Kasabach-Merrit
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61
Q

What percentage of ITP becomes chronic?

A

20%, usually older girls. Chronic = >6m duration

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62
Q

What is the risk of severe bleed in ITP?

A

<3%

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63
Q

When do you treat ITP, and what with?

A

IVIG, platelet transfusion (for ICH/surgery), tranexamic acid, steroids 2/52 (need BM first to r/o malignancy as steroids can mask this)
Treat if: mucosal bleeding, haematuria, ICH/head trauma, retinal haemorrhage, urgent surgery required

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64
Q

Causes of low platelets in neonate

A

Alloimmune (NAIT) - maternal antibodies to father’s platelet antigen. HPA1 most common (85%). Can occur in firstborn. If plts <20 urgent transfuse due to risk ICH first few days life +/- IVIG
Isoimmune (ITP) - mother will have low platelets
Sepsis
TORCH infection

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65
Q

What drugs can cause thrombocytopenia?

A 
Sodium valproate
Phenytoin
Carbamazepine 
Cotrimoxazole
Rifampicin
Heparin
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66
Q

Signet rings within cells

A

Malaria

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67
Q

Howell-Jolly bodies

A

Hyposplenism

nuclear remnants in RBC that haven’t been removed as spleen is not working

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68
Q

Basophilic stipling

A

Lead poisoning

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69
Q

Auer rods

A

AML, needle-like

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70
Q

Blue cytoplasm with pink/red granules

A

AML

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71
Q

Vitamin-K dependant clotting factors

A

10, 9, 7, 2

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72
Q

Extrinsic coagulation cascade activated by?

A

Tissue thromboplastin

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73
Q

Prothrombin time measures?

A

Extrinsic pathway + common pathway

Factor 7, 5, 10, 2

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74
Q

APTT measures?

A

Intrinsic pathway + common pathway

Factor 8, 9, 11, 12, 5, 10, 2

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75
Q

Thrombin time measures?

A

Final part of common pathway (thrombin, fibrinogen -> fibrin). Prolonged by lack of fibrinogen, and inhibitors (heparin, fibrin degradation products)

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76
Q

Natural anticoagulants

A

Antithrombin III - most potent inhibitor, heparin potentiates its effect
Protein C - inhibits 5a, 8a, promotes fibrinolysis. Protein S enhances protein C action

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77
Q

Describe haemophilia A

A
  • X-linked, 30% new mutations
  • Carriers have variable F8 levels
  • Neonate - IVH, haematoma, excessive bleeding
  • Inc APTT, N PT + bleeding time, F8 decr
  • Mild: DDAVP (releases vWF from endothelial cells, incr FVIII)
  • Tranexamic acid for mucosal bleeds
  • Mod+severe: recombinant F8, up to 20% develop inhibitors (low titre give inc doses F8. High titre use F7a to
    bypass)
  • Mild 5-50% F8, mid 1-5%, severe <1%
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78
Q

Role of vWF?

A

Stabilises and protects F8 from proteolytic enzymes, mediates platelet adhesion. Produced by endothelial cells and platelets

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79
Q

Describe vWD

A
  • F8 decr, vWF decr, bleeding time N or inc, plt count N, ristocetin cofactor activity decr (c.f. normal in Haemophilia A)
  • T1: AD, 70%, partial def of vWF
  • T2: abnormal vWF function, AD
  • T3: complete absence, AR, present similarly to Haemophilia A
  • Tx: DDAVP, tranexamic acid, type2b+3 require plasma-derived factor 8 concentrate (recombinant factor contains no vWF)
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80
Q

Describe Vit K deficiency of infancy

A
  • Early, within 24 hrs, maternal warfarin or anticonvulsants
  • Classic, 1-7d, idiopathic or BF babies
  • Late, >8d, peaks several weeks, idiopathic or due to BFing, malabsorption, diarrhoea
  • APTT inc, PT inc, plt + fibrinogen N. PT prolonged only when <50% prothrombin
  • Tx: IV Vit K, works within 20 min, don’t given IM. FFP +/- transfusion
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81
Q

Presentation of F13 deficiency

A

Delayed bleeding from umbilicus/cord in up to 80%.
AR. Decr F13 level. Clots lyse in 5M urea solubility test.
Causes inability to cross-link fibrinogen. Lifelong risk ICH. Tx: fibrogammin.
Abnormalities of fibrinogen also present as cord oozing

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82
Q

Li Fraumeni predisposes to?

A

Soft tissue sarcoma, brain tumours

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83
Q

Von Hippel-Lindau predisposes to?

A

Cerebellar haemangioblastomas, renal cell carcinoma, pheochromocytoma

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84
Q

Beckwith-Wiedemann predisposes to?

A

Hepatoblastoma, neuroblastoma. rhabdomyosarcoma. adenocortical carcinoma

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85
Q

Ataxia telangectasis predisposes to?

A

Leukaemia, B-cell lymphoma

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86
Q

Which chemotherapy drugs does not interact with DNA directly?

A

Vincristine

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87
Q

Describe lupus anticoagulant

A
  • Acquired inhibitors that may produce a prolonged APTT
  • Common, frequently associated with recent viral infections
  • Transient
  • They are not a risk for bleeding. Increased risk thrombosis
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88
Q

Highest risk for testicular relapse in leukaemia is in?

A

T cell ALL > B cell ALL

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89
Q

Describe Kasabach-Merritt syndrome

A
  • Giant haemangioma leading to localised form of DIC
  • Lesions at birth in approximately 50% of patients
  • Severe thrombocytopaenia, hypofibrinogenemia, elevated fibrin degradations products and fragmentation of red blood cells
  • Consumptive coagulopathy
  • Tx: corticosteroids, interferon alpha, surgery, embolisation and chemo (vincristine, cyclophosphamide and actinomycin D)
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90
Q

What does cryoprecipitate contain?

A

Factor 8, fibrinogen, vWF, factor 13

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91
Q

What is the composition of ALL?

A

> 80% B-cell (pre-B or common ALL), 15% T-cell, 2% mixed

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92
Q

What is the most powerful adverse prognostic factor in ALL?

A

Poor response to induction chemo

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93
Q

Significance of presenting WCC count?

A

If high then poor prognosis in B-cell ALL

Not prognostic in T-cell ALL

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94
Q

What is the Philadelphia translocation, and what can improve its prognosis?

A

t(9;22), improved by tyrosine kinase inhibitors (imatinib)

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95
Q

Why do you give IT chemo?

A

As CNS lymphoblasts protected from systemic chemotherapy by blood-brain barrier

96
Q

What is the current cure rate for ALL?

A

> 90%

97
Q

Describe AML

A
  • Poorer prognosis than ALL, 5yr survival 60-65%, T21
  • Cytarabine-based chemo over 6m, intensive
  • Skin involvement (purple spots), HSM, bleeding, masses (chloroma), gum hypertrophy
  • Chromosomal abnorm in 80%. t(8;21) has good prognosis
  • FLT3-ITD poor, NPM1 favourable, CEBPA favourable
  • Film: blasts, auer rods, basophilic (blue) cytoplasm with pink granules
  • APML can be treated with arsenic + retinoic acid
98
Q

Significance of acute promyelocytic leukaemia?

A

May present with DIC. Induction chemo with retinoic acid and arsenic (if presenting WCC <10). Good prognosis if survive induction.

99
Q

Where do NHL and HL originate from?

A

NHL from B or T lymphocytes, whereas HL from B lymphocytes

100
Q

What are B symptoms?

A

Paraneoplastic phenomena.

Night sweats, weight loss (>10% in 6m), fever >38

101
Q

What are the pathognomonic cells in Hodgkin’s lymphoma?

A

Reed-Sternberg cells - large cell, multiple nuclei, “owl-like” appearance

102
Q

Describe Hodgkin’s lymphoma

A
  • Inc 2nd decade life, F>M (except <10y), can be EBV-driven
  • Painless cervical LAN most frequent symptom
  • May have prolonged history, mediastinal mass, itch, rash, B-symptoms
  • Ann Arbor staging. PET scan v. useful
  • Usually >90% survival
103
Q

Describe non-Hodgkin’s lymphoma

A
  • Group of conditions including Burkitt and lymphoblastic
  • Burkitt most common NHL in childhood - fast growing
  • Lymphoblastic 80% T cell, 20% B cell
  • Abdomen (B cell) > mediastinum (T-cell) > head/neck
  • St-Judes staging
  • More rapid onset than HL
  • B cell malignancy use rituximab (binds to CD20+ve B cells, destroys them) and intensive chemo
104
Q

Describe Burkitt lymphoma

A
  • Commonest NHL in childhood, fast-growing, usually high stage at presentation, prognosis >85% even for stage 4
  • Often present with abdominal mass
  • Usually short history, doubling time = hours
  • Burkitt cells = dark blue cytoplasm with white vacuoles
105
Q

Who is at most risk of tumour-lysis syndrome?

A

High count ALL (esp T cell)
B cell NHL (esp Burkitt)
Elevated uric acid/LDH pre-treatment
As have potential for bulky disease - a high cell mass that undergoes lysis with treatment.
Risk peaks at 24-48hrs post starting treatment.

106
Q

What are the findings in tumour-lysis syndrome?

A

Uric acid and phosphate crystals leading to acute renal failure, fluid overload, high phos, high K, high urea, high creat, low calcium

107
Q

Treatment of tumour-lysis syndrome?

A

Hyperhydration (2 x maint)
Urate oxidase (rasburicase, increases urinary excretion) or allopurinol (give in G6PD)
Treat hyperkalaemia
May need dialysis
Avoid calcium replacement while phosphate is rising as causes complexes that worsen renal damage

108
Q

What is the most common brain tumour in children?

A

Low-grade glioma (astrocytoma)

109
Q

What is the classic finding on histology of low-grade glioma?

A

Rosenthal fibres

110
Q

What is the most common type of astrocytoma?

A

Juvenile pilocytic astrocytoma
2/3rds found in posterior fossa
Most tumours involving optic pathway are JPA

111
Q

Treatment of low grade glioma?

A

Observation, may regress/have indolent course. May require surgery +/- chemo (vincristine, carboplatin).
Intervene if visual deterioration.
(B_RAF pathway aberration.
Better prognosis in children with NF1.

112
Q

Describe high grade glioma

A

Locally infiltrative astrocytic tumours
Glioblastoma multiforme, poor prognosis, no recent change in survival outcomes
Surgery + chemoradiation. Temozolamide standard of care in adults

113
Q

Most common malignant brain tumour?

A

Medulloblastoma, makes up 20% of brain tumours.

Highly malignant primitive neuroectodermal tumour

114
Q

Location of medulloblastoma

A

Typically arise from vermis of cerebellum. Up to 1/3rd metastasised at presentation.

115
Q

Prognosis of medulloblastoma?

A

Depends on stage, histology, molecular markers
PF survival 75-80% at 5 years.
Most favourable = desmoplastic/nodular subtype
Least favourable = large cell/anaplastic
Infants <3y - high risk/poor prognosis as cannot have radiotherapy to brain

116
Q

Treatment of medulloblastoma?

A

Surgery, craniospinal irradiation with boost, adjuvant chemotherapy (vincristine, cisplatin, cyclophos)
Spinal MRI + LP for CSF cytology 2/52 post surgery to check for residual cells.

117
Q

Describe atypical teratoid/rhabdoid tumours

A
  • Highly malignant, short history
  • Usually <3y/o
  • Disseminated disease in 20%, anywhere along brain and spine, often short clinical history
  • Median survival 9-12 months
  • Biallelic inactivation of SMARCB1 in majority (prev hSNF5), INI loss in tumour
  • Tx: surgery, chemo, radiation
118
Q

What gene mutation is associated with atypical teratoid/rhabdoid tumours?

A
  • Biallelic inactivation of SMARCB1 in majority (prev hSNF5), INI expression loss in tumour
  • Screen family, at risk of renal and soft tissue tumours if germline mutation present
119
Q

Describe ependymomas

A
  • Arise from wall of ventricle or spinal canal
  • Not chemosensitive. Use surgery and radiotherapy
  • Poor prognosis if: <3y/o, anaplastic, posterior fossa primary, metastasis
120
Q

Describe brainstem gliomas

A
  • 10-20% of brain tumours, 80% are diffuse brainstem gliomas
  • Poor prognosis, <10% survival
  • Cannot resect. Chemo nil benefit. Use radiotherapy
  • Avoid biopsy as high risk (diagnosis via MRI)
121
Q

Presenting signs of diffuse intrinsic pontine glioma?

A
  • Cerebellar signs (ataxia, dysarthria)
  • CN palsies
  • Long tract signs (hypertonic, hyperreflexic, upgoing plantars, motor deficit)
  • MRI: T2 hyperintense, T1 hypointense, >50% pons, basilar artery encasement
122
Q

Describe craniopharyngiomas

A
  • Suprasellar region
  • Raised ICP, visual disturbances, pituitary dysfunction, GH insufficiency, bitemporal hemianopia
  • Usually benign but morbidity due to tumour and treatment (endocrine)
  • Often calcified on imaging
  • Tx surgery and chemo
123
Q

What can cause posterior reversible encephalopathy syndrome?

A

Chemotherapy + hypertension

  • Headache, confusion, seizures
  • Often visual loss due to occipital lobe involvement
  • Usually vasogenic oedema, lack of diffusion restriction on MRI
124
Q

Describe diencephalic syndrome

A
  • Failure to thrive associated with hypothalamic/optic chiasm tumours, usually pilocytic astrocytoma
  • Severe emaciation despite normal caloric intake
  • Nystagmus, occ blindness, raised ICP
  • Possible hyperactivity, hyperalertness, euphoria and vomiting
125
Q

Describe the inheritance of retinoblastoma

A
  • 60% sporadic, unifocal, onset ~18m
  • 40% hereditary, AD, multifocal, onset ~10m. Deletion of tumour suppressor gene, germline RB1 mutation in Ch13q14. Still requires inactivation of remaining allele at cellular level.
126
Q

Describe retinoblastoma

A
  • Presents with leukocoria (white eye reflex), strabismus, nystagmus, red eye
  • 60% sporadic, 40% hereditary
  • Hereditary - can have trilateral tumour (inc pineal region)
  • Tx: laser, cryotherapy, chemo (vincristine, carboplatin). If poor prognostic features (such as anterior segment) then enucleation.
  • Cure > globe salvage > vision
127
Q

RB1 mutation

A
  • Retinoblastoma, trilateral tumour (bilateral retinoblastoma + pineal gland)
  • AD
  • Increased risk osteosarcoma, soft tissue sarcoma, breast cancers
  • Avoid CT scans and unnecessary radiation
128
Q

What cells does neuroblastoma arise from?

A

Primordial neural crest cells in adrenal medulla and sympathetic chain

129
Q

Discuss neuroblastoma

A
  • Average age 18m
  • FHx in 1-2% (PHOX2B gene, central hypoventilation, Hirschprung’s)
  • Can have raccoon eyes (bony invasion), metabolic symptoms due to catecholamine secretion (sweating, pallor, diarrhoea, hypertension), bone pain/refusal to walk, Horner’s syndrome, Opsoclonus/myoclonus, blue subcutaneous nodules
  • MRI, iodine 123 MIBG scan (uptake in 90%), urinary catecholamines (HVA and VMA)
130
Q

Where are neuroblastoma located?

A

65% abdomen, 35% adrenal glands, 20% chest, pelvis 4%, neck 1%

131
Q

Factors associated with poor prognosis in neuroblastoma

A

Increased age, increased stage, molecular biology (diploid, 11q abnormality, MYCN amplification - growth oncogene)

132
Q

Dinutuximab use and mechanism

A

Anti-GD2 antibody (Ch14.18) used for high risk neuroblastoma.
Kills cells expressing GD2 including peripheral nerves and CNS cells, ciliary eye muscles - severe neuropathic pain, sensory/motor neuropathy. Often need morphine. Sepsis-like picture with fever, oedea.

133
Q

Treatment of neuroblastoma

A

Depends on risk.
Observe (some involute spontaneously), surgery, chemo, radiotherapy, may need stem cell rescue, dinutuximab (anti-GD2 antibody) for high risk

134
Q

Describe Wilm’s tumour (nephroblastoma)

A
  • 90% of all renal tumours
  • Predisposed by nephrogenic rest (foetal tissue persisting into infancy)
  • Abdo mass (75%), haematuria, constipation, pain, HTN
  • Metastasises to lungs and IVC tumour thrombus
  • Low risk - surgery+observe. Other risk - surgery + chemo +/- radiotherapy
  • Overall survival >90% for favourable disease
135
Q

Describe osteosarcoma

A
  • Most common bone tumour, peak 2nd decade life
  • 80% lesions extremity (distal femur, prox tibia, humerus), 50% in knee joint
  • Rapid bone growth/metaphysis areas
  • Pain and mass
  • Inc risk with radiation, RB1 gene, Li-Fraumeni
  • 15-20% metastasis, esp lungs
  • May have inc LDH and ALP
  • Pathology: osteoblastic, chondroblastic
  • Radiotherapy not used. Surgery + chemo (micrometastatic disease)
136
Q

Describe Ewing’s sarcoma

A
  • Small, round cell tumour of neuroectodermal origin
  • Bone or soft tissue (extraskeletal ES), equal distribution axial (pelvis, ribs, vertebra) + long bones, diaphysis
  • Pain, soft tissue mass, constitutional symptoms (fever, weight loss, high WCC+ESR)
  • Recurring translocation involving EWSR1 gene (22q12, t11;22)
  • 25% present with mets (<30% EFS)
  • Sensitive to radiotherapy. Surgery + chemo.
137
Q

Side effects of steroids

A 
Immunosuppression
Weight gain
Weakness
Psychosis or mood swings
Stomach ulcers
Elevated blood pressure
Sleep problems
Hyperglycaemia
Osteoporosis
Avascular necrosis
138
Q

Describe the features of Langerhans Cell Histiocytosis

A
  • Presents with cutaneous lesions (trunk and scalp) resembling cradle cap, which is caused by infiltrates of Langerhans cells
  • Hepatosplenomegaly, lymphadenopathy, pulmonary lesions, destructive osteolytic bone lesions (punched out xray)
  • Anaemia, thrombocytopenia
  • Recurrent infections such as otitis media and mastoiditis
  • Bilateral proptosis due to retro-orbital accumulation of granulation tissue
139
Q

Describe the blood film in haemolytic uraemic syndrome

A

Films characteristically show schistocytes/helmet cells as well as thrombocytopenia.
Triad of microangiopathic haemolytic anaemia, thrombocytopenia, acute renal failure.

140
Q

Describe the x-ray differences between osteosarcoma and Ewing’s sarcoma

A

Osteosarcoma - lytic and sclerotic lesion, “sunburst”

Ewing’s sarcoma - lytic lesion with periosteal reaction, “onion skin”

141
Q

What is the half life of F8 and F9?

A

F8 - 12 hours

F9 - 24 hours

142
Q

Causes of acquired methaemaglobinaemia

A

Lidocaine/lignocaine, nitrous oxide, metoclopramide.
Methaemoglobin is formed when the iron in haemoglobin is oxidised to its ferric state. This reduces the oxygen carrying capacity of haemoglobin.
Inc risk infants.

143
Q

What is the most common prothrombotic abnormality:

  • Acquired
  • Familial
A 
Acquired = antiphospholipid antibodies (lupus anticoagulant and cardiolipin antibodies)
Familial = Factor V Leiden mutation = activated protein C resistance. Heterozygotes also have inc. risk clotting by 3-8%
144
Q

What is the difference between embryonal and alveolar rhabdomyosarcoma?

A
  • Embryonal - more common, younger, better prognosis
  • Alveolar - less common, older, worse prognosis. Fusion gene is pathognomonic (PAX7 better prognosis than PAX3-FOXO1, t(1;13), 2(2;13))
145
Q

Where are malignant germ cell tumours located?

A

Gonadal (30%) or extra-gonadal (70%) - sacrococcygeal, retroperitoneal, mediastinum, neck, pineal region

146
Q

Describe malignant germ cell tumours

A
  • Gonadal 30%, extra-gonadal 70%
  • Range from undifferentiated embryonal carcinoma to mature teratoma (pure teratoma has no metastatic potential)
  • Gonadal: testicular swelling, pelvic pain, ovarian torsion, abnormal or precocious puberty
  • Inc risk with undescended testes
  • Ix: AFP and b-HCG
  • Can metastasise to lungs, liver lymph nodes
  • Tx depends on type/location. Chemo is cisplatin and carboplatin
147
Q

Risk factors for hepatoblastoma

A

Premature infants with IUGR, Beckwith-Wiedemann syndrome, FAP, congenital retinal pigmentation

148
Q

Hepatoblastoma vs. HCC

A

Hepatoblastoma - underlying normal liver, much higher AFP than in HCC. Usually <2y/o
HCC - usually underlying liver disease, metastatic HCC is incurable but can have palliative chemo

149
Q

Presentation and treatment of hepatoblastoma

A

Usually painless abdominal mass
Treatment with chemo (cisplatin, carboplatin) and surgery (resection or transplant)
Overall survival 70-80%

150
Q

What is a Langerhans cell?

A

A potent antigen presenting cell.

151
Q

Describe the process of Langerhans cell histiocytosis

A
  • Clonal accumulation and proliferation of abnormal Langerhans cells in bone marrow. Forms damaging infiltrates with eosinophils, lymphocytes and normal histiocytes.
  • Can affect any age
  • Localised disease (skin, bone, lymph nodes) or multi-system (worse prognosis)
  • Unpredictable course, can rapidly progress
  • Can develop diabetes insipidus
  • Tx: steroids, vinblastine, methotrexate, mercaptopurine
152
Q

What are the symptoms of haemophagocytic lymphohistiocytosis?

A

Fever, hepatosplenomegaly, cytopenia, high lipids, coagulopathy, high fibrinogen and ferritin, liver dysfunction, neuro symptoms, inc CD25

153
Q

What is the difference between primary and secondary HLH?

A
  • Primary/familial - AR, defect in NKC and cytotoxic T cell function, results in phagocyte and inflammation activation - multi-system pathology
  • Secondary = macrophage activation syndrome - usually precipitated by infection, rheumatological, malignancy, immune deficiency
154
Q

Treatment of HLH

A

Steroids, chemotherapy, bone marrow transplant as definitive treatment

155
Q

What are the indications for a bone marrow transplant?

A
  • Malignancy - high risk leukaemia, or rescue due to high dose chemo e.g. neuroblastoma, brain tumours
  • Metabolic - to replace missing/defective enzyme e.g. mucopolysaccharidosis
  • Immunodeficiency e.g. SCID
  • Bone marrow failure e.g. aplastic anaemia, Fanconi anaemia
  • Haemoglobinopathy e.g. Sickle cell, thalassaemia major
156
Q

Name the cancer predisposition syndromes

A
  • Familial retinoblastoma - germline RB1 mutation
  • Li-Fraumeni - mutation in tumour suppressor TP53
  • Neurofibromatosis 1 - mutation of NF-1 gene on Ch 17
  • Beckwith-Wiedemann - mutation in 11p15.5
  • Trisomy 21
157
Q

Cancer predisposition: retinoblastoma

A
  • Germline RB1 mutation, Ch13q14, deletion of tumour suppressor gene
  • AD, high penetrance
  • Requires double hit
  • Trilateral tumour (bilat retino + pineal pNET)
  • In risk osteosarcoma, soft tissue sarcoma, breast cancer
158
Q

Cancer predisposition: Li-Fraumeni

A
  • Mutation in TP 53 gene, tumour suppressor gene
  • AD, FHx early-onset cancers
  • Soft tissue sarcoma, osteosarcoma, breast cancer, leukaemia, brain cancers
159
Q

Cancer predisposition: neurofibromatosis 1

A
  • Mutation of NF-1 on Ch17, unable to produce neurofibromin
  • AD, variable expression
  • Neurofibroma, schwannoma, optic glioma, astrocytoma, pheochromocytoma, malignant tumour from plexiform neurofibroma
160
Q

Cancer predisposition: Beckwith-Wiedemann

A
  • 85% sporadic, mutation in 11p15.5 causing over-reactivity of IGF2 gene
  • From abnormal methylation or uniparental disomy
  • 10-20% develop malignancy - hepatoblastoma, neuroblastoma, Wilm’s, rhabdomyosarcoma, adrenocortical tumour
  • Abdo USS 3-monthly until age 8, AFP every 6 weeks until age 4
161
Q

Cancer predisposition: Trisomy 21

A
  • ALL (inc 20x), acute megakaryoblastic leukaemia (inc 500x)
  • Transient myeloproliferative disease
  • 10-20% of these develop AMKL within 3 years
162
Q

Describe transient myeloproliferative disease

A
  • Trisomy 21, occurs in first 3m life in 10% (develops in utero), resolves spontaneously in 1-3m.
  • Anaemia, thrombocytopenia, hepatosplenomegaly. Inc WCC and blasts.
  • Inc risk (10-20%) of then developing acute megakaryoblastic leukaemia
163
Q

Acquired causes of thrombophilia

A
  • Central line e.g. neonates - renal vein thrombosis
  • Trauma, surgery, nephrotic syndrome, malignancy
  • Lupus anticoagulant (in APTT) e.g. SLE, antiphospholipid syndrome
164
Q

Inherited causes of thrombophilia

A
  • Factor V Leiden mutation - activated protein C resistance
  • Protein S, protein C deficiency - AD, infants with homozygous protein C def present like DIC, can cause purpura fulminans and blindness. FFP only source of protein C. When out of neonatal period, use warfarin
  • Prothrombin gene mutation - inc prothrombin levels
165
Q

Factor V Leiden mutation

A
  • Activated protein C resistance, so factor 5+8 become more resistant to inactivation by protein C
  • Inc risk VTE, no inc risk arterial thrombosis
  • Heterozygotes 5x inc risk, homozygotes 50x inc risk
  • 4% Caucasians carriers
166
Q

When and why does the nadir of Hb occur?

A

Around 2 months age, due to decr EPO (inhibited by inc O2 after birth, once Hb is at nadir get decr O2 therefore EPO stimulated again and get rise in Hb).

167
Q

Extrinsic vs intrinsic haemolysis

A
  • Extrinsic (acquired)

- Intrinsic (congenital)

168
Q

Rod cells on blood film

A

= pencil cells

Iron deficiency anaemia

169
Q

Causes of macrocytosis

A

B12/folate deficiency
Newborn
Hypothyroidism
Down syndrome
Massive reticulocytosis (recticulocytes are larger than RBC)
Liver disease
Megaloblastic anaemia - nutritional, IEOM, drug e.g. azathioprine

170
Q

B12/folate deficiency

A

Macrocytic anaemia
Mild thrombocytopenia +/- neutropenia (hypersegmented)
- Dietary deficiency, defective absorption (small intestine, intrinsic factor), inc requirement (growth, skin disease)

171
Q

Causes of extra-vascular haemolysis

A
  • Immune, DAT+ve - auto or alloimmune. Get splenomegaly

- Non-immune (congenital) e.g. thalassaemia, haemoglobinopathy, hereditary spherocytosis, G6PD def

172
Q

Cold agglutination

A
  • Antibodies bind to RBC causing haemolysis, IgM, DAT-ve. - Usually lab phenomenom only, not clinically relevant in body as warmer.
  • Post-viral, can be associated with mycoplasma
  • C3 coating on FBCs
  • Intravascular haemolysis
  • Spurious macrocytosis
  • Doesn’t respond to steroid
173
Q

Causes of intra-vascular haemolysis

A
  • Infection - malaria, sepsis, burns
  • Cardiac e.g. prosthetic valves
  • Microangiopathic - TTP, HUS, Kasabach Meritt, calcineurin inhibitors (ciclosporin, tacrolimus), malignancy, DIC
174
Q

HbF
HbA
HbA2

A

HbF - alpha 2, gamma 2
HbA - alpha 2, beta 2
HbA2 - alpha 2, delta 2

175
Q

Unfractionated heparin vs LMW heparin

A
  • Unfractionated - short 1/2 life, based on APTT ratios, infants metabolise UFH faster, reversed by protamine sulphate. ADRs bleeding, HIT, osteporosis
  • LMWH - less monitoring required (anti-X1 levels), decr risk HIT and osteoporosis, effect not readily reversed by protamine sulphate
176
Q

Side effects of warfarin

A

Bleeding, tracheal calcification, hair loss

- Management of bleeding with IV Vit K, FFP, recombinant F7a

177
Q

Threshold for acute leukaemia on bone marrow?

A

Need >20% blasts (normal number blasts <5%), but often is >85%. High nucleus:cytoplasm ratio

178
Q

Good prognosis in ALL?

A

Hyperdiploid >50
Trisomy 4, 10
ETV6-RUNX1 t(12;21) translocation - 99% cure rate

179
Q

Significance of Philadelphia Chromosome?

A

t(9;22) balanced translocation resulting in fusion of BCR and ABL1 creating a novel tyrosine kinase resulting in unregulated cell proliferation.

  • Imatinib /dasatinib (TK inhibitor) has dramatically improved outcome.
  • Survival >70%
180
Q

Discuss the use of Blinatumomab

A
  • Early phase studies, promising results for multiple relapse/refractory B-ALL. BiTE molecule - binds CD19+ve B cells and T cells at each end. Non cytotoxic, T cell kills B cell
  • Can lead to cytokine release syndrome (similar to MAS) and neurotoxicity
181
Q

Infant ALL

A

Often high WCC at presentation, MLL (11q23) rearrangements, rare disorder, poor outcome, inc treatment-related toxicity

182
Q

Describe risks of hyperleukocytosis

A
  • WCC>100 (or >50 in AML). More frequent AML, higher mortality
  • Leukostasis in brain and lungs, like pulm oedema
  • DIC
  • Tumour lysis syndrome
  • Tx: hyperhydration, rasburicase, may need leukapheresis, manage DIC, avoid transfusion (inc viscosity)
183
Q

Differentials of mediastinal masses - ant, middle, post

A
  • Anterior: lymphoma, germ cell tumour
  • Middle: lymph nodes, TB, fungal, osteosarcoma
  • Posterior: neuroblastoma, neurofibroma, schwannoma
  • 80% of mediastinal masses are malignant
184
Q

Describe superior mediastinal compression syndrome

A
  • SVC obstruction - facial plethora, oedema, collaterals chest wall
  • Airway obstruction
  • Horner’s syndrome
  • T cell leukaemia, lymphoma, GCT
  • Tx: avoid GA/lying flat, MDT involvement, steroids to shrink tumour/swelling, may need anticoagulation
185
Q

Symptoms and treatment of spinal cord compression (3-5% of children at diagnosis)

A
  • Local or radicular pain
  • Motor weakness, sensory loss, incontinence
  • Paraplegia/quadriplegia can progress rapidly
  • Emergency MRI, surgery/radio/chemo +/- dexamethasone
186
Q

Anaphylaxis most commonly with which chemotherapies?

A
  • L’Asparaginase/PEG asparaginase
  • Etoposide
  • Carboplatin
  • Amifostine
  • Platelet transfusions
    + newer moncolonal antibodies
  • Premedications given to reduce risk
187
Q

Drugs that are high risk if extravasation occurs?

A

Doxorubicin, dactinomyin, danorubicin, vinblastine, vincristine

188
Q

Febrile neutropenia and shock.. organism is?

A

Gram-ve sepsis until proven otherwise (e.g. E.Coli, pseudomonas, Klebsiella).
If shocked use tazocin + vancomycin + amikacin
(if cisplatin exposure then avoid amikacin due to hearing loss, can use meropenem instead).

189
Q

Febrile neutropenia in on high dose cytarabine…organism is most likely?

A

Alpha haemolytic strep (strep mitis/strep pleuridans)

Always treat these patients with tazocin + vancomycin

190
Q

Anti-emetics used in oncology

A 
Ondansetron, metoclopramide
Scopoderm patch
Arepitant
Cyclizine
Lorazepam
Dexamethasone (half dose if using arepitant. Don't use if on immunotherapy)
191
Q

Drugs associated with mucositis?

A

Methotrexate, doxorubicin

192
Q

Risk of cardiomyopathy with?

A

Doxorubicin (anthracyclines) + thoracic radiation

  • worse with puberty, pregnancy, high intensity exercise
  • Give dexrazoxane (iron chelator) prior to anthracycline in high risk patients
193
Q

Risk of pulmonary penumonitis/fibrosis with?

A

Bleomycin, lung irradiation

194
Q

Highest risk of infertility with which cancer?

A

Ewing’s sarcoma

195
Q

Risks of second malignancy post cancer treatment

A
  • Radiation exposure: sarcoma
  • Etoposide/alkylator (cyclophos, ifosfamide) exposure: AML, sarcoma
  • Hodgkin (mantle irradiation): breast (30-40% risk by age 50) and thyroid cancer
196
Q

Risk of high frequency hearing loss with which chemo?

A

Cisplatin (up to 70%), worse if had gentamicin, amikacin, frusemide treatment. 30% require hearing aids.

197
Q

Environmental factors linked to cancer development in children

A
  • Treatment for previous cancers (radiotherapy, etoposide + alkylating chemo)
  • Lack of infection exposure may play a role in ALL
  • EBV in nasopharyngeal carcinoma
198
Q

MYC is associated with?

MYCN is associated with?

A 
MYC = Burkitts lymphoma 
MYCN = neuroblastoma
199
Q

Klinefelters have an increased risk of?

A

Mediastinal germ cell tumour

Breast cancer

200
Q

Primary function of a checkpoint inhibitor is?

A

Activation of T-cells and dendritic cells

201
Q

Familial adenomatous polyposis (FAP)

A

AD cancer predispostion syndrome caused by germline mutations in the tumour suppressor gene APC. Inc risk bowel cancer and hepatoblastoma

202
Q

Types of cancer mutations

A

Errors in:

  • Oncogenes promote healthy growth-> cause constant activation
  • Tumour suppressor genes suppress excess growth -> excess growth
  • DNA repair genes proof read and repair mutations -> don’t fix errors
203
Q

Which malignancies can metastasise to bone marrow?

A

Ewing’s, alveolar rhabdomyosarcoma, neuroblastoma

204
Q

Side effects of 6-mercaptopurine?

A

Hepatic necrosis

Myelosuppression

205
Q

Posterior fossa syndrome

A
  • Delayed neuro changes after tumour resection
  • Cerebellar mutism, irritability, difficulty verbalising, emotional lability, nystagmus
  • 20% incidence after resection medulloblastoma
  • Mutism resolves ~1m, other neuro symptoms persist
206
Q

CNS manifestations of Tuberous Sclerosis

A
  • Supependymal giant cell astrocytoma (5-15%) - benign, slow growing, wall lateral ventricles
  • Cerebral cortical tubers (90-100%), associated with seizures
  • Subependymal hamartomatous nodules
  • Everolimus is the standard of care for unresectable lesions
207
Q

Describe the mechanism of methotrexate

A
  • Competitively inhibits the enzyme folic acid reductase
  • Interferes with tissue cell reproduction
  • Main effect is directed to the S phase of cell division. - Actively proliferating tissues such as malignant cells, bone marrow, buccal and intestinal mucosa are sensitive to the effects of methotrexate
  • Need normal kidney function to excrete the drug adequately and avoid excessive toxicity
208
Q

What mutation is implicated in the majority of hereditary spherocytosis cases?

A

Mutations in the ANK1 gene is implicated in more than 50% of cases of HS

209
Q

What is the life-span of WBC, RBC, neuts, platelets?

A

RBC 120 days, WBC 13-20 days, platelets 10 days, neuts 5 days

210
Q

How does rasburicase work?

A

Oxidation of uric acid to allantoin

211
Q

Prognostic factors in neuroblastoma?

A

Good prognostic factors:
Age <1
Hyperdiploidy
Whole chromosome gains

Poor prognostic factors:
Amplification of the N-myc proto-oncogene
Segmental chromosome abnormalities

212
Q

Describe transfusion-related GvHD

A
  • Is a result of donor T lymphocytes directed against recipient tissue antigens.
  • Fever, rash, diarrhoea, LFT derangement and pancytopenia.
  • It has a very poor prognosis and is almost universally fatal
  • Prevent by using irradiated blood.
213
Q

What are schistocytes?

A

Schistocytes are seen in microangiopathic haemolytic anaemia (such as haemolytic uraemia syndrome). They are caused by damage in the vessels. Schistocytes are always an abnormal finding on a blood film.

214
Q

What are haemophilia factor inhibitors?

A
  • High affinity IgG antibodies
  • 30% Haem A, 5% Haem B (may have anaphylaxis)
  • Decrease the response to factor infusions and also delay maturation (bone age, puberty, growth).
  • Tx: switch from prophylactic factor to on demand factor.
  • Immune tolerance induction may also be performed. - Activated FVII can used to bypass inhibitors but it is less effective (misses thrombin amplification) and can predispose to thrombosis
  • If there is upcoming surgery then use recombinant FIIa q2hourly for the preceding 48 hours.
  • New medication emicizumab is a monoclonal AB that can bridge IX and X to bypass VIII and may be used as a prophylaxis alternative.
215
Q

By how much do platelets and Hb rise after transfusion?

A

Each unit of platelets will raise the platelet count by 20x10g/L.
Each unit of red cells will raise the count by 10x10g/L

216
Q

How much iron does each unit of RBC contain?

A
  • Each unit of RBC contains 250mg of iron
  • Clinically significant iron overload is thought to occur after 10 units in children
  • Iron chelator such as desferroxamine can be used.
    The RDI for iron is
  • 11mg/day infants
  • 7-10mg/day children
217
Q

Intra vs extravascular haemolysis

A
  • Intra: higher LDH and haemoglobinuria, low hapto (HUS, DIC, transfusion reaction, PNH, prosthetic valve hemolysis)
  • Extra: higher bilirubin, lower LDH, low hapto (alloimmune haemolysis, thalassaemia, inc RBC turnover).
218
Q

What is Bernard Soulier Syndrome?

A
  • AR disorder
  • Absence or severe deficiency of the VWF receptor (GPIb complex) on the platelet membrane
  • Extremely large platelets and thrombocytopenia, prolonged bleeding time
  • Defect in ristocetin induced agglutination, but platelets will agglutinate in response to other agonists (collagen, ADP)
219
Q

What are the findings in sideroblastic anaemia?

A
  • Hypochromic, microcytic red cells mixed with normal red cells = dimorphic population
  • Extremely high red cell distribution width
  • Serum iron elevated, transferrin saturation increased
  • Impaired heme synthesis leads to retention of iron within the mitochondria
  • Sideroblasts on film with ferritin iron deposits (blue stained) in the mitochondria of erythroid precursors, forming an apparent ring around the nucleus
220
Q

What is the only known heritable risk factor for brainstem gliomas?

A

NF1

221
Q

What are types of small round blue cell tumours?

A
  • Medulloblastoma
  • Neuroblastoma
  • Ewings
  • Wilms
  • Hepatoblastoma
  • Retinoblastoma
222
Q

What syndromes are associated with medulloblastoma?

A

Gorlin (nevoid basal cell carcinomas), Li-Fraumeni (TP53, wide range of cancers), Turcot (adenomatous colon polyps), Gardner (FAP), Cowden (multiple hamartomas)

223
Q

Discuss the use of Car-T cell therapy

A
  • Engineered autologous T cells transfected using viral vector, target CD19+ve B cells + destroys them
  • Currently used in CD19+ve ALL, rationale for use in B-cell lymphoma
  • Cells are autologous, therefore rejection minimal, can persist in circulation leading to prolonged remission, can penetrate into CSF
  • Can cause cytokine release syndrome in up to 50% cases, long-term B cell depletion requiring immunoglobulin
224
Q

What are the different MHC Class I antigens?

A

HLA-A, HLA-B, HLA-C

Found on nearly all cells (except erythrocytes and corneal endothelium)

225
Q

What are the different MHC Class II antigens?

A

HLA-DR, HLA-DQ, HLA-DP

Found on antigen-presenting cells (e.g. dendritic cells, B-lymphocytes, monocytes, macrophages, Langerhans cells)

226
Q

Discuss parent/sibling relationship with HLA markers for HSCT

A
  • Parents are haploidentical matches to patient (i.e. patient shares 50% with Dad, 50% with Mum)
  • 1/4 chance of having a complete HLA matched sibling
227
Q

What are the different types of HSCT?

A
  • Autologous (self) vs allogeneic (foreign)
  • Related vs unrelated
  • Matched vs mismatched/unmatched
228
Q

What does a haploidentical transplant mean?

A
  • Refers to the use of a parent or non-matched sibling as the donor
  • A child by definition will have at worst 50% of a parent’s HLA type
229
Q

In what conditions will excess haematopoietic stem cells be produced in the body?

A
  • Umbilical cord blood (physiological)
  • Marrow regeneration following myelosuppressive chemotherapy
  • Following GCSF stimulation
230
Q

What are the advantages and disadvantages of cord blood for HSCT?

A

+ve: risk-free collection, less requirement for HLA matching, low risk infection, low incidence GvHD, rapid availability, better for metabolic syndromes + AML
-ve: low volume/dose, slow engraftment, no possibility of donor lymphocyte infusion

231
Q

What are the advantages and disadvantages of bone marrow for HSCT?

A

+ve: moderately brisk engraftment, moderate risk GvHD, donor lymphocyte infusion available
-ve: increased risks of collection, contains all marrow cells including RBC

232
Q

What are the advantages and disadvantages of peripheral blood stem cells for HSCT?

A

+ve: rapid engraftment, low risk during collection, only CD34+ cells collected, donor lymphocyte infusion available
-ve: increased rates GvHD, especially chronic GvHD, not a practical procedure on small patient

233
Q

What is the order of preference for allo-SCT sources?

A
  • Matched sibling donor - marrow
  • Matched sibling donor - PBSC
  • Matched unrelated donor - marrow
  • Matched unrelated donor - cord
  • Mismatched family donor (haploidentical) - marrow
234
Q

Waiting time for stem cell transplants depending on type used?

A
  • Matched unrelated donor - 4-6 weeks
  • Cord bloods - 7-14 days
  • Haploidentical donors (parents) - immediately
235
Q

What is the role of transplant conditioning?

A
  • Allows treatment of underlying condition (in malignancy)
  • Immunosuppression to allow engraftment (in allotransplant)
  • Can be: myeloablative (higher risk of toxicity) or reduced intensity conditioning (higher risk of graft failure)
236
Q

Discuss cause and management of graft versus host disease

A
  • Acute = <100 days post transplant
  • 1: damage to host tissues by inflammation from preparative chemo/radio regime
  • 2: recipient and donor APC trigger activation of donor-derived T cells
  • 3: T-cell mediated cytotoxicity against target host cells. Production of cytokines and TNFa causing further inflammation and damage
  • Signs: rash, jaundice, diarrhoea = all correlate with severity of GvHD
  • Tx: optimise immunosuppression - cyclosporine, steroids, sirolimus/tacrolimus
  • Chronic GvHD very difficult to manage, rare complication

Paediatrics RACP written exam (18 decks)

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