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HEMA Lab > OFT > Flashcards

OFT Flashcards

1
Q

T/F: Osmotic Fragility Test is still performed in the laboratory

A

FALSE: OST is an obsolete test (not performed)

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2
Q

It measures the ability of RBC to resist osmotic stress

A

OFT

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3
Q

It is the movement of a substance from high to a low concentration until it reaches equilibrium

A

Osmosis

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4
Q

Traditional and non-specific test for hereditary spherocytosis

A

OFT

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5
Q

OFT is a traditional and non-specific test for _______________

A

Hereditary spherocytosis

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6
Q

Sample in OFT

A

Heparinized whole blood

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7
Q

T/F: Sample in OFT is performed immediately (STAT)

A

True

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8
Q

What will happen to the RBC if sample is prolonged?

A

Spherocytic

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9
Q

Principle: RBCs are surrounded by a selectively permeable membrane that allows the exchange of __________ and ____________. If the RBCs are placed in a __________ saline solution, ______ ___________ will be established by drawing water into the cells, which then swell.

A

Gases and electrolytes

Hypotonic

Osmotic equilibrium

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10
Q

Considerations:

More water present inside the cell compared to the water outside the cell

A

Hypertonic

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11
Q

Considerations:

Hypertonic causes the cell to _______

A

shrink/shrinking

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12
Q

Considerations:

No changes in the shape of the cell (with central pallor), Balanced

A

Isotonic

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13
Q

Considerations:

Number of water that exits the cell = Number of water that enters the cells

A

Isotonic

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14
Q

Considerations:

More water outside the cell compared to the inside of the cell

A

Hypotonic

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15
Q

Considerations:

Hypotonic causes ________ to the RBC

A

Swelling (spherocytic)

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16
Q

Considerations:

Swelling of RBC in hypotonic will result in __________?

A

Bursting

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17
Q

Materials in OFT

A
  • 05% NaCl
  • Wassermann tubes (12)
  • Test tube rack
  • Gum label
  • Pasteur pipette
  • Parafilm
  • Timer
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18
Q

The method in OFT

A

GRIFFIN-SANFORD METHOD

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19
Q

GRIFFIN-SANFORD METHOD:

Prepare ____% of NaCl solution

A

0.5

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20
Q

GRIFFIN-SANFORD METHOD

Arrange ___ wasserman test tubes in the rack. Label them from 25 down to 14 (left to right)

A

12

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21
Q

Deliver 0.5% NaCl in each tube drop using a ___________

A

Pasteur pipette

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22
Q

T/F: The number of drops of distilled water should correspond to the number labeled on each tube.

A

False: The number of 0.5% NaCl

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23
Q

T/F: The number of drops of distilled water should bring the total volume of the solution in each tube to 25.

A

True

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24
Q

Tube #25

0.5% NaCl:
Distilled water:
% sol’n:

A

25

0

0.5%

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25
Q

Tube #24

0.5% NaCl:
Distilled water:
% sol’n:

A

24

1

0.48%

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26
Q

Tube #23

0.5% NaCl:
Distilled water:
% sol’n:

A

23

2

0.46%

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27
Q

Tube #22

0.5% NaCl:
Distilled water:
% sol’n:

A

22

3

0.44%

28
Q

Tube #21

0.5% NaCl:
Distilled water:
% sol’n:

A

21

4

0.42

29
Q

Tube #20

0.5% NaCl:
Distilled water:
% sol’n:

A

20

5

0.40%

30
Q

Tube #19

0.5% NaCl:
Distilled water:
% sol’n:

A

19

6

0.38%

31
Q

Tube #18

0.5% NaCl:
Distilled water:
% sol’n:

A

18

7

0.36%

32
Q

Tube #17

0.5% NaCl:
Distilled water:
% sol’n:

A

17

8

0.34%

33
Q

Tube #16

0.5% NaCl:
Distilled water:
% sol’n:

A

16

9

0.32%

34
Q

Tube #15

0.5% NaCl:
Distilled water:
% sol’n:

A

15

10

0.30%

35
Q

Tube #14

0.5% NaCl:
Distilled water:
% sol’n:

A

14

11

0.28%

36
Q

GRIFFIN-SANFORD METHOD

Allow the tubes to stand for _______ at room temperature

A

2 hours

37
Q

RESULT:

Faintly pink supernatant WITH red cell sediments

A

Initial Hemolysis

38
Q

RESULT:

Fluid is red WITH NO red cell sediments

A

Complete Hemolysis

39
Q

REFERENCE VALUE:

Initial Hemolysis: _________ (Tube no. ____)

A

0.44 土 0.02%

22

40
Q

REFERENCE VALUE:

Complete Hemolysis: _________ (Tube no. ____)

A

0.32 土 0.02%

16

41
Q

COMPUTATION:

T/F: Mas mababa yung concentration ng NaCl = more isotonic

A

False: more hypotonic

42
Q

RBC lacking central pallor

A

Spherocytes

43
Q

Seen in hereditary spherocytosis

A

Spherocytes

44
Q

Due increased hemoglobin concentration, therefore (decreased, increased) surface area to volume ratio

A

decreased

45
Q

T/F: Konti water lang kaya occupy ng cell = easier to hemolyze

A

True

46
Q

SPHEROCYTES

___ OFT
___ MCHC

A

Increased
Increased

47
Q

T/F: Result is affected by the surface area-to-volume ratio of RBC

A

True

48
Q

Increased surface area-to-volume ratio = (decreased, increased) OFT

A

decreased

49
Q

What is/are seen in Decreased OFT

A
  • Normal RBC
  • Sickle Cells/Drepanocytes
  • Target Cells
50
Q

Sickle Cells/Drepanocytes is resistant to _________________

A

Plasmodium falciparum

51
Q

Target cells a.k.a

A

Codocytes

52
Q

Decreased surface area-to-volume ratio = (decreased, increased) OFT

A

increased

53
Q

What is/are seen in Increased OFT

A

Spherocytes

54
Q

Increased Osmotic Fragility

A
  • Hereditary Spherocytosis
  • Burns
  • Immune Hemolytic Anemia (Swelling of RBC)
55
Q

Decreased Osmotic Fragility

A
  • Thalassemia
  • Target cells
  • Sickle Cell Anemia
  • Iron Deficiency Anemia
56
Q

⅓ central pallor = (high, low) surface area to volume ratio

A

High

57
Q

Normal cell: ___ of central pallor

A

1/3

58
Q

INITIAL HEMOLYSIS (%NaCl)

NORMAL

A

0.46
(Tube 22)

59
Q

INITIAL HEMOLYSIS (%NaCl)

Hereditary Spherocytosis

A

0.65
(Tube 25)

60
Q

INITIAL HEMOLYSIS (%NaCl)

Thalassemia

A

0.35
(Tube 17)

61
Q

________ is characterized by numerous target cells

A

Thalassemia

62
Q

INITIAL HEMOLYSIS (%NaCl)

Sickle cell anemia

A

0.35
(Tube 17)

63
Q

COMPLETE HEMOLYSIS
(%NaCl)

Normal

A

0.35-0.30
(Tube 17-14)

64
Q

COMPLETE HEMOLYSIS
(%NaCl)

Hereditary Spherocytosis

A

0.45
(Tube 22)

65
Q

COMPLETE HEMOLYSIS
(%NaCl)

Thalassemia

A

0.20
(Beyond tube 14)

66
Q

COMPLETE HEMOLYSIS
(%NaCl)

Sickle cell anemia

A

0.20
(Beyond tube 14)

HEMA Lab (1 decks)

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