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OFT Flashcards

1
Q

T/F: Osmotic Fragility Test is still performed in the laboratory

A

FALSE: OST is an obsolete test (not performed)

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2
Q

It measures the ability of RBC to resist osmotic stress

A

OFT

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3
Q

It is the movement of a substance from high to a low concentration until it reaches equilibrium

A

Osmosis

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4
Q

Traditional and non-specific test for hereditary spherocytosis

A

OFT

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5
Q

OFT is a traditional and non-specific test for _______________

A

Hereditary spherocytosis

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6
Q

Sample in OFT

A

Heparinized whole blood

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7
Q

T/F: Sample in OFT is performed immediately (STAT)

A

True

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8
Q

What will happen to the RBC if sample is prolonged?

A

Spherocytic

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9
Q

Principle: RBCs are surrounded by a selectively permeable membrane that allows the exchange of __________ and ____________. If the RBCs are placed in a __________ saline solution, ______ ___________ will be established by drawing water into the cells, which then swell.

A

Gases and electrolytes

Hypotonic

Osmotic equilibrium

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10
Q

Considerations:

More water present inside the cell compared to the water outside the cell

A

Hypertonic

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11
Q

Considerations:

Hypertonic causes the cell to _______

A

shrink/shrinking

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12
Q

Considerations:

No changes in the shape of the cell (with central pallor), Balanced

A

Isotonic

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13
Q

Considerations:

Number of water that exits the cell = Number of water that enters the cells

A

Isotonic

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14
Q

Considerations:

More water outside the cell compared to the inside of the cell

A

Hypotonic

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15
Q

Considerations:

Hypotonic causes ________ to the RBC

A

Swelling (spherocytic)

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16
Q

Considerations:

Swelling of RBC in hypotonic will result in __________?

A

Bursting

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17
Q

Materials in OFT

A
  • 05% NaCl
  • Wassermann tubes (12)
  • Test tube rack
  • Gum label
  • Pasteur pipette
  • Parafilm
  • Timer
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18
Q

The method in OFT

A

GRIFFIN-SANFORD METHOD

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19
Q

GRIFFIN-SANFORD METHOD:

Prepare ____% of NaCl solution

A

0.5

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20
Q

GRIFFIN-SANFORD METHOD

Arrange ___ wasserman test tubes in the rack. Label them from 25 down to 14 (left to right)

A

12

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21
Q

Deliver 0.5% NaCl in each tube drop using a ___________

A

Pasteur pipette

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22
Q

T/F: The number of drops of distilled water should correspond to the number labeled on each tube.

A

False: The number of 0.5% NaCl

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23
Q

T/F: The number of drops of distilled water should bring the total volume of the solution in each tube to 25.

A

True

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24
Q

Tube #25

0.5% NaCl:
Distilled water:
% sol’n:

A

25

0

0.5%

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25
Tube #24 0.5% NaCl: Distilled water: % sol'n:
24 1 0.48%
26
Tube #23 0.5% NaCl: Distilled water: % sol'n:
23 2 0.46%
27
Tube #22 0.5% NaCl: Distilled water: % sol'n:
22 3 0.44%
28
Tube #21 0.5% NaCl: Distilled water: % sol'n:
21 4 0.42
29
Tube #20 0.5% NaCl: Distilled water: % sol'n:
20 5 0.40%
30
Tube #19 0.5% NaCl: Distilled water: % sol'n:
19 6 0.38%
31
Tube #18 0.5% NaCl: Distilled water: % sol'n:
18 7 0.36%
32
Tube #17 0.5% NaCl: Distilled water: % sol'n:
17 8 0.34%
33
Tube #16 0.5% NaCl: Distilled water: % sol'n:
16 9 0.32%
34
Tube #15 0.5% NaCl: Distilled water: % sol'n:
15 10 0.30%
35
Tube #14 0.5% NaCl: Distilled water: % sol'n:
14 11 0.28%
36
GRIFFIN-SANFORD METHOD Allow the tubes to stand for _______ at room temperature
2 hours
37
RESULT: Faintly pink supernatant WITH red cell sediments
Initial Hemolysis
38
RESULT: Fluid is red WITH NO red cell sediments
Complete Hemolysis
39
REFERENCE VALUE: Initial Hemolysis: _________ (Tube no. ____)
0.44 土 0.02% 22
40
REFERENCE VALUE: Complete Hemolysis: _________ (Tube no. ____)
0.32 土 0.02% 16
41
COMPUTATION: T/F: Mas mababa yung concentration ng NaCl = more isotonic
False: more hypotonic
42
RBC lacking central pallor
Spherocytes
43
Seen in hereditary spherocytosis
Spherocytes
44
Due increased hemoglobin concentration, therefore (decreased, increased) surface area to volume ratio
decreased
45
T/F: Konti water lang kaya occupy ng cell = easier to hemolyze
True
46
SPHEROCYTES ___ OFT ___ MCHC
Increased Increased
47
T/F: Result is affected by the surface area-to-volume ratio of RBC
True
48
Increased surface area-to-volume ratio = (decreased, increased) OFT
decreased
49
What is/are seen in Decreased OFT
- Normal RBC - Sickle Cells/Drepanocytes - Target Cells
50
Sickle Cells/Drepanocytes is resistant to _________________
Plasmodium falciparum
51
Target cells a.k.a
Codocytes
52
Decreased surface area-to-volume ratio = (decreased, increased) OFT
increased
53
What is/are seen in Increased OFT
Spherocytes
54
Increased Osmotic Fragility
- Hereditary Spherocytosis - Burns - Immune Hemolytic Anemia (Swelling of RBC)
55
Decreased Osmotic Fragility
- Thalassemia - Target cells - Sickle Cell Anemia - Iron Deficiency Anemia
56
⅓ central pallor = (high, low) surface area to volume ratio
High
57
Normal cell: ___ of central pallor
1/3
58
INITIAL HEMOLYSIS (%NaCl) NORMAL
0.46 (Tube 22)
59
INITIAL HEMOLYSIS (%NaCl) Hereditary Spherocytosis
0.65 (Tube 25)
60
INITIAL HEMOLYSIS (%NaCl) Thalassemia
0.35 (Tube 17)
61
________ is characterized by numerous target cells
Thalassemia
62
INITIAL HEMOLYSIS (%NaCl) Sickle cell anemia
0.35 (Tube 17)
63
COMPLETE HEMOLYSIS (%NaCl) Normal
0.35-0.30 (Tube 17-14)
64
COMPLETE HEMOLYSIS (%NaCl) Hereditary Spherocytosis
0.45 (Tube 22)
65
COMPLETE HEMOLYSIS (%NaCl) Thalassemia
0.20 (Beyond tube 14)
66
COMPLETE HEMOLYSIS (%NaCl) Sickle cell anemia
0.20 (Beyond tube 14)

HEMA Lab (1 decks)

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