Nucleic Acids Flashcards
Avery-MacLeod-McCarty
Phage T2
Hershey-Chase Experiment
Nucleotides are the…
Involved in redox reactions: ?? and ??
Energy transfer process…
Intracellular signalling:
Biosynthetic reactions:
High-energy molecules:
The structure of the monomer determines the…
Polynucleotides
Nucleotide Structure
Numbering Purines + Pyramidines
where are the nitrogens in pyrimidines
where are nitrogens on purines
Adenosine
Cytosine
Thymidine
Guanosine
Uracil and Thymine
In pyrimidines (uracil, thymine, and cytosine), N_ is linked to…the sugar
In purines (adenine and guanine), N_
is linked… to the sugar
In nucleosides where are phosphates attatched
In the shorthand version (rAMP or dAMP), without the r it is assumed it is…
Purines: Adenine and Guanine naming
Pyrimidines: Cytosine, Thymine, and Uracil naming
how to name nucleotides
Phosphoester Bonds
Phosphodiester Bonds
Phosphoanhydrides
what creates directionality in phosphodiester bonds
Nucleic acids are asymmetrical…
what are the 5’ and 3’ ends named for
The primary structure for a nucleic acid is the sequence of…
The net charge of the nucleic acid is…
The backbone consists of sugar and phosphates, both of which are…
Nucleotide Phosphate Naming
RNA Alkaline Hydrolysis
Cytosine Deaminates to Form…
Properties of Nucleotide Bases
Aromaticity causes electron delocalization which…
Structure of DNA
B-Form DNA Conformation
Reading DNA Strands
DNA Denaturation or Melting
DNA Melting Absorbance
hyperchromacity and hypochromacity
Denaturation of Double Stranded DNA Speed
DNA Denaturation and Renaturation
Renaturation
Melting Curves and Base Composition
pH and Melting Curves
Salt Concentration and Melting Curves
DNA Hybridization
RNA forms Different Structures than DNA
RNA is stabilized by the same forces as DNA:
DNA and RNA Double Stranded Helix
RNA Melting Curve
RNA asborbance