november mocks Flashcards
what is an enzyme
a biological catalyst
how does temperature affect enzyme function
as temperature increases the enzyme and substates have more kinetic energy which means they move faster so have more successful collisions
once temperature has got to a certain point the temperature breaks the bonds that hold together the amino acids (which make the proteins) which changes the shape of the enzyme.
this is denaturing
once the enzyme has denatured the substrate can no longer fit in the active site (as it has lost its shape) meaning that the reaction will stop
practical - investigate how enzyme activity can be effected by changes in temperature
amylase digests starch
- mix 10cm of 10% starch solution with 5cm of 5% amylase in a boiling tube.
- heat in a water bath
- every minute add 1 drop of this solution to 1 drop of iodine in a spotting tile
- repeat using different temps of water baths
when the starch has been fully digested (so none is present) iodine will stay orange
how is enzyme function affected by changes in pH
If the pH is too high or too low, the bonds that hold the amino acid chain together to make up the protein can be disrupted/destroyed
This will change the shape of the active site, so the substrate can no longer fit into it, reducing the rate of activity
Moving too far away from the optimum pH will cause the enzyme to denature and activity will stop
active transport
the movement of molecules from a low to high concentration using ATP
plants use active transport in their root hair cells to absorb mineral ions.
organisms have special carrier proteins in the cell membrane. These use ATP to provide the energy to move the substances across the membrane against the concentration gradient.
diffusion
the random movement of particles from a high to a lower concentration
so particles of O2 will move out of the lungs into the RBCs as the lower conc of O2 is in the RBCs
osmosis
the movement of water molecules from a high potential to a lower potential across a partially permeable membrane
how does surface area to volume ratio affect movement of substances in and out of cells
A larger surface area speeds up the rate of diffusion as there are more opportunities for the molecules to move, which is why surfaces such as alveoli in lungs are so large. Surface area to volume ratio is more significant, as the two counteract (oppose) each other: an efficient exchange surface has a surface area which is very large compared to the distance the molecules must travel. SA:V is increased when structures are small.
how does distance affect movement of substances in and out of cells
Diffusion takes longer if the molecules have to travel further. Therefore cells are small (smaller volume reduces distance).
how does concentration gradient affect movement of substances in and out of cells
If there is a very large difference in concentration between to areas, molecules will diffuse from the higher to the lower concentration quickly. If the concentration gradient (difference) is small, diffusion will happen more slowly.
how does temperature affect movement of substances in and out of cells
At higher temperatures, molecules have more kinetic energy and so move faster.
practical - investigate diffusion and osmosis using living systems
- Make a 5 different concentration of sucrose solutions
- Measure 5cm3 of each dilution into separate test tubes.
- Use a cork borer to cut out six potato chips and cut down the sections into identically sized chips. Dry each chip using a paper towel to remove excess
water but do not squeeze. - Weigh each before the start of the experiment.
- Place a potato chip in each test tube (one per sucrose concentration) and leave
for 20 minutes. - Remove each potato chip, dry gently using paper towel, and weigh them in turn.
- Calculate the percentage change in mass for each sucrose solution.
practical - investigate diffusion and osmosis using non-living systems
- Add sucrose solution to a section of Visking tubing – a selectively permeable substance used to model a cell membrane.
- Weigh the Visking tubing and its contents.
- Add the Visking tubing to a beaker of water.
- Leave for 1 hour.
- Pat the Visking tubing dry to remove excess water.
- Reweigh the Visking tubing and its contents.
order of organisation
organelle - a component within a cell that carries out a specific task (mitochondria)
cell - basic functional and structural units in a living organism
tissues - a group of cells of similar structure working together to perform a particular function
organs - made from a group of different tissues working together to perform a particular function
organ system - made from a group of organs with related functions, working together to perform body functions within the organism
nucleus
controls the cells activity (by making proteins)
contains the chromosomes (strands of DNA which carry genes which code for a protein)
cell membrane
boundary between the cytoplasm and the cell’s surrounding.
controls what substances enter and exit the cell.
cytoplasm
jelly-like liquid where reactions occur
mitochondria
carries out some aerobic respiration, which produces ATP
ribosomes
synthesize (assemble) proteins and amino acids
chloroplasts
contain chlorophyll, absorb light energy and use it to carry out chemical reactions of photosynthesis making biological molecules for plants.
cell wall
helps keep plants in a fixed shape
vacuole
filled with a water liquid called cell sap, stores dissolved sugars, mineral ions and other substances.
similarities of plants and animal cells
nucleus
cytoplasm
mitochondria
cell membrane
ribosomes
differences of plants and animal cells
plants only:
cell wall (cellulose)
vacuole
chloroplasts
what happens in the mouth
mechanical + chemical digestion + swallowing
mechanical - food is broken down into smaller molecules by chewing. this increases SA for enzymes and prevents discomfort when swallowing
chemical - saliva is released by the salivary glands. saliva makes food easier to swallow and it contains amylase
swallowing - before swallowing food is shaped into a ball and pushed to the back of the mouth by the tongue. this ball is called a bolus. there is a flap called epiglottis which blocks food from entering the trachea
what is chemical digestion
food broken down into smaller soluble molecules by enzymes, bile and acids
what is mechanical digestion
food broken down via physical methods such as churning, grinding and chewing
what happens in the oesophagus
long tube that connects the mouth and the stomach. the bolus is pushed down/through by peristalsis
what is peristalsis
the gut muscles contracting and relaxing to form a wave to push the bolus down/through the oesophagus.
circular muscles contract + longitudinal muscles relax
circular muscles relax + longitudinal muscles contract
what happens in the stomach
the gastric glands in the stomach walls secrete pepsin which starts to digest protein
contractions of the stomach wall causes the contents to mix maximizing the contact between the enzymes and food
the stomach is acidic because HCl is released from the gastric glands as the optimum pH for pepsin is acidic. the low pH would burn through the stomach walls so they are covered in mucus to prevent this. the HCl also kills most bacteria and fungi present in the food.
what happens in the small intestine
both digestion and absorption happen in the small intestine
made up of duodenum and ileum
what happens in the duodenum
the final place of chemical digestion.
the pancreas makes several enzymes and secretes them into the duodenum.
trypsin, amylase, lipase
the duodenum also contains glands which secrete the enzymes they produce into the duodenum
maltase, peptidase
the duodenum also contains bile
what is bile
bile is produced by the liver and stored in the gall bladder
- neutralizes the stomach acid because the duodenum enzymes work best at 7-8 pH
- emulsifies lipids - breaks down the large droplets into smaller droplets, increasing SA for lipase to digest the fat
what happens in the ilium
absorption begins. the small soluble molecules are absorbed. some by diffusion but some such as glucose by active transport.
how is the ilium optimized for diffusion
large SA - folding of the ileum, villi & microvilli (folds on the surface of cells lining the villi) increase SA
short diffusion distance - the villi cells are one cell thick
high concentration gradient - provided by capillary network and lacteals removing absorbed molecules
what happens in the colon
site of all reabsorption of water
what happens the rectum
the faeces are stored in the rectum and egested from the anus
what does the pancreas do
produces and secretes amylase, trypsin, lipase into the duodenum
secretes an alkaline fluid into the duodenum to neutralize the acidity of the stomach
what is the large intestine
colon and rectum
how is the small intestine adapted for absorption
villi and micro villi
very long which increase SA and time for diffusion and active transport
peristalsis mixes food together and keeps things moving
starch ->
starch –(amylase)–> maltose
maltose ->
maltose –(maltase)–> glucose
protein ->
protein –(pepsin)–> peptides
lipid ->
lipid –(lipase)–> glycerol and 3 fatty acids
how do living organisms produce ATP
respiration!!
how do cells get energy to carry out their life processes
ATP from respiration
peptides ->
peptide –(peptidase)–> amino acids
what is the difference between anaerobic and aerobic respiration
aerobic respiration requires O2 whereas anaerobic respiration does not
aerobic respiration completely breaks down glucose whereas anaerobic respiration does not
aerobic respiration releases a lot of energy whereas anaerobic respiration only produces a little
what is cell respiration
cells constantly break down food molecules to produce ATP
this happens continuously because without it the cell would have no energy and die