nov ppes Flashcards
bio paper 1 (ends at flashcard 77) and some paper 2
Give the differences between light and electron microscopes.
- Light microscopes are cheap to make, electron microscopes are expensive
- You can see the outlines of cells using a light microscopes
- You can see the finer details including organelles using an electric microscope
- Electron microscopes have a greater resolving power
- Electron microscopes have a higher resolution
What is the equation for magnification?
image size (actual size) / object (cell) size
How do you convert from micrometres (μm) to millimetres (mm)?
Divide by 1000.
What are the two main groups of cells?
- Eukaryotic cells (e.g. animal, plant)
- Prokaryotic cells (e.g. bacteria)
Where is DNA stored in eukaryotic cells?
The nucleus.
Where is DNA stored in prokaryotic cells?
In the plasmid (a loop of DNA)
Name the 5 organelles that animal and plant cells share.
- Cell membrane (semi-permeable, controls what enters and leaves)
- Nucleus (DNA)
- Mitochondria (respiration)
- Cytoplasm
- Ribosome (protein synthesis)
Name 3 organelles unique to plant cells.
- Chloroplasts (chlorophyll, photosynthesis)
- Cell wall (cellulose)
- Permanent vacuole (stores sap)
How do bacteria reproduce?
Binary fission.
Explain the process of making cultures with aseptic technique.
- Lift lid of petri dish towards flame (sterilising air)
- Put a drop of culture on agar and spread evenly
- Put drops of antibiotics on culture if needed
- Place a few bits of tape (allowing space for aerobic respiration between tape) on lid and dish to hold the lid closed
- Incubate at 25°C
- Measure size of cultures/areas with no bacteria with πr2
How many chromosomes does every human cell have?
23 pairs (46 in total), apart from gametes (eggs/sperm) which have only 23.
Describe the process of mitosis.
- The nucleus dissolves and genetic material is duplicated. The two sets of chromosomes move to different sides.
- Mitochondria, ribosomes and other organelles are duplicated.
- The cell divides, producing two genetically identical diploid cells (cells with 23 pairs of chromosomes).
- New nuclei are formed.
What is a stem cell?
A cell that hasn’t specialised yet.
Where are stem cells found?
- Animal embryos
- Plant meristems
- Bone marrow (this stem cells can only specialise into blood cells)
Name and explain one process used to make more stem cells.
Embryonic cloning: These embryo clones can be made of a person to harvest stem cells from.
- The developing animal embryo is then split apart many times before the cells of the embryo become specialised
- This forms many separate embryos that are genetically identical
- These cells can be used to treat conditions without the cells being rejected
What else can cloning be used for?
The preservation of species or producing crops with desired traits.
Define diffusion.
Diffusion is the movement of particles from an area of high concentration to low concentration. It doesn’t require energy and so, passive.
Define osmosis.
The movement of water particles from area of high concentration to low concentration through a semi-permeable membrane to balance the concentrations of a solution inside/outside a cell.
Name 3 things that can increase the rate of diffusion.
- The difference in concentrations
- Surface area
- Temperature
Describe the osmosis practical.
- Weigh then place identical potato cylinders in sugar solutions of varying concentrations
- After a set time (24 hours) remove excess water and reweigh the cylinders
- Calculate the percentage change in mass using the following equation:
(final mass - initial mass / initial mass) * 100 - Plot the percentage change in mass against concentrations. Where the line of best fit crosses the x-axis is which concentration should result in no osmosis. Meaning, the concentration of sugar in the sugar solution is the same as the concentration of sugar in the potato cylinder.
Define active transport.
The movement of particles through a membrane via carrier proteins. This requires energy because the particles need to move against the concentration gradient.
What is the order of organisation?
Cell -> tissue -> organ -> organ system
Explain the digestive system.
- Teeth breaks down food mechanically. Saliva contains amylase, an enzyme which breaks down carbohydrates (starch and sugar)
- It travels down the esophagus to the stomach, containing hydrochloric acid and enzymes (amylase, lipase and protease) that chemically break down the food.
- The live produces bile which is stored in the gall bladder; it’s used in the small intestine to emulsify lipids forming droplets and increasing their surface area
- The pancreas secretes amylase which breaks down starch into glucose in the small intestine
- Water is absorbed into the bloodstream in the large intestine
- Nutrients like glucose are absorbed into the bloodstream by the villi in the small intestine (starch is too large)
What is an enzyme?
Special proteins that act as biological catalysts. They aren’t ever used up.
They’re specific and so only break down substrates that fit their active site (‘lock and key’ principle)