Normal Histology Flashcards
How do you histologically recognize the liver
Solid tissue, characterized by cuboidal cells in lines
May or may not see cortex of thin connective tissue
Little C.T. , so not good for trichrome stains
How do you histologically recognize the spleen
Solid tissue like liver, H and E, stains dark
Various cell types, notice patches of colors at high power differentiating white and red pulp
How do you histologically recognize the brain
Solid tissue, look for characterictic grey and white matter, and Gyri (round, curving areas)
Cerebellum is also helpful
Difficult to get a good cut, so keep trying
How do you histologically recognize the kidney
Remember, nephron- look for glomeruli
Appear as nest like structures, near cortex (outside)
Also easy to ID renal pelvis
How do you histologically recognize the intestines
In general, all should be cross-sectional, and have a lumen
Duodenum, jejenum and ileum all resemble each other, just differentiate by looking for peyers patches, length of villi..etc…
How do you histologically identify the colon
Slightly different with larger villi, and more prominent muscularis layer for larger peristaltic action
How do you histologically identify other tubular structures
Differentiate uterus from esophagus/trachea from intestinal structures
Uterus has more CT, and large endometrium, myometrium
Uterus Great for trichrome
How do you histologically recognize esophagus/trachea
Usually can pick up cartilage cells on trachea, large and clear.
Esophagus often collapses
Usually the 2 are together
How do you histologically identity lymph nodes
Solid structures, with white and red pulp like spleen
Differentiate by shape, and amount of red pulp in circles
how do you histologically identify glands
All glands resemble each other by having acini filled with something
Differentiating salivary versus thyroid versus prostate gland is challenging.
How do you histologically identify ovaries and testis
Look for characterized cells of each
Ovaries have ova at different stages,
CL is also easy to see
Look for the hilus
How do you histologically identify testis
Look for seminiferous tubules which take up most of the structure, and will have sperm in them
How do you histologically identify the esophagus
This is esophagus, longitudinal section. Note the stratified squamous epithelium
how do you histologically identify muscle
Striated muscle at high power- all look the same, lots of elastic fibers
