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bacte lesson 21 > Nonfermenting and Miscellaneous Gram (-) Bacilli > Flashcards

Nonfermenting and Miscellaneous Gram (-) Bacilli Flashcards

1
Q

Gen characteristics of nonfermenting & miscellaneous gram (-) bacilli.

A
  • Not able to ferment sugar
  • Fail to acidify O-F media (when overlayed w/mineral oil
  • fail to acidify TSI
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2
Q

Oxidize carbohydrates

A

Oxidizers

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3
Q

Inert/biochemically inactive

A

Nonoxidizer/asacchrolytic

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4
Q

Nonfermenting and miscellaneous gram (-) bacilli characteristics except:
A. Motile
B. Most oxidase (-)
C. Pigmentation
D. Ubiquitous

A

B. Most oxidase (+)

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5
Q

In OF test, what is the color of oxidative and non oxidizer

A

Oxidative(+): yellow
Non oxidizer(-): green

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6
Q

Thin, gram (-)bacilli/coccobacilli

A

Nonfermenting/miscellaneous gram (-) bacilli

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7
Q

T or F
Nonfermenting/miscellaneous gram (-) bacilli is oxidase (+)

A

True

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8
Q

Nonreactivity in 24hrs. in commercial system for ___.

A

Enterobacteriacieae

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9
Q

T or F
Nonfermentose produce acid in the slant/butt of TSI/KIA.

A

False- no acid formation

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10
Q

T or F
Nonfermentose is resistant to a variety of antimicrobial agents.

A

True

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11
Q

List of Clinically Significant Nonfermentative Gram-Negative Bacilli

A
  • Fluorescent Pseudomonad Group
  • Acinetobacter
  • Stenotrophomonas maltophilia
  • Burkholderia
  • Moraxella and Oligella
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12
Q

Characteristics of Pseudomonas except.
A. Catalase and Oxidase (-)
B. Motile
C. Grows in MAC(lactose fermenter)
D. Oxidizer/ asaccharolytic

A

A. Catalase and Oxidase(+)
C. Non lactose fermenter

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13
Q

Most common spp. Of Pseudomonas

A

P. aeruginosa

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14
Q

It cause nosocomial respiratory tract infection.

A

P. aeruginosa

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15
Q

Clin. Infection of P. aeruginosa except:
A. Nosocomial UTI
B. Bacteremia w/ecthyma gangrenosum of skin
C. Encephalitis
D. Endocarditis

A

C

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16
Q

Arrange the order from most to least common cause of gram (-) bacilli bacteremia.

A
  1. E. coli
  2. K. pneumoniae
  3. P. aeruginosa
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17
Q

Other less conditions of P. aeruginosa

A

Otitis externa (swimmer’s ear)
Necrotizing skin rash (jacuzzi/hot tub syndrome
Infection of nail beds (wearing artificial nails)

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18
Q

Virulence factors of P. aeruginosa

A

Endotoxin (LPS)
Pili
Capsule
Motility
Exotoxin
Exotoxin A

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19
Q

Most important virulence factor of P. aeruginosa cause it block protein synthesis.

A

Exotoxin A

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20
Q

Fluorescent pseudomonad group.

A

P. aeruginosa
P. flourescens
P. putida

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21
Q

Fluorescent pseudomonad group produce ___.

A

Pyoverdin (yellow-green/ yellow-brown)

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22
Q

P. aeruginosa produce ____ (blue, water soluble pigment)

A

Pyocyanin

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23
Q

Pyocyanin+pyoverdin =

A

Blue green /green color

24
Q

Other pigments produced by Fluorescent pseudomonad group.

A

Pyorubin (red)
Pyomelanin(brown/black)

25
Q

Agar recommend for Fluorescent pseudomonad group.

A

Cetrimide agar

26
Q

T or F
Fluorescent pseudomonad group is beta hemolytic in SBA.

A

False-nonhemolytic

27
Q

Appearance of Colonies of Fluorescent pseudomonad group.

A

Flat spreading colonies (metallic sheen)

28
Q

P. aeruginosa produce what type of odor

A

Fruity, grapelike odor

29
Q

The odor produce by P. aeruginosa is because of ___.

A

2-aminoacetophenone

30
Q

Fluorescent pseudomonad group best grow at what temp?

A

42°C (thermophiles)

31
Q

Fluorescent pseudomonad group that hs very low virulence

A

P. fluorescens
P. putida

32
Q

P. fluorescens and P. putida can grow at ____°C

A

4°C

33
Q

They produce pyoverdin

A

P. fluorescens
P. putida

34
Q

How to differentiate P. fluorescens and P. putida

A

Gelatin hydrolysis

35
Q

T or F
In gelatin hydrolysis
(+) P. putida
(-) P. fluorescens

A

False.
(-) P. putida
(+) P. fluorescens

36
Q

Most common spp. Of Acinetobacter

A

A. baumannii
A. lwoffii

37
Q

Acinetobacter characteristics except:
A. Opportunistic pathogen
B. Second common to P. aeruginosa
C. Oxidase(+)
D. Catalase (-)
E. Nonmotile

A

C.Oxidase (-)
D.Catalase (+)

38
Q

Acinetobacter prefers what temp and pH to grow.

A

Lower temp. (30-35°C)
pH 5.5 to 6.0

39
Q

T or F
Acinetobacter is strictly aerobic

A

True

40
Q

Stenotrophomonas maltophilia formely known as _____

A

P. maltophilia

41
Q

3rd most common nonfermenting gram (-) bacilli

A

S. maltophilia

42
Q

Common to hospital environment

A

S. maltophilia

43
Q

Single most important risk factor infection of S. maltophilia

A

Venous Catheter

44
Q

Stenotrophomonas maltophilia is
Oxidase ()
Catalase ()

A

O(-)
C(+)

45
Q

Low-grade, nosocomial pathogens

A

Burkholderia cepacia

46
Q

Most often associated with pneumonia in patients with (chronic granulomatous disease)

A

B. cepacia

47
Q

Slective media for Burkholderia cepacia:
-PC
-OFPBL
-BCSA

A

(P. cepacia)
(Oxidative-fermentive base, polymyxin B, Bacitracin)
(B. cepacia selective agar)

48
Q

Appearance of B. cepacia in BCSA.

A

weak, slow, oxidase (+) rxn.
Produce nonfluorescing yellow/green pigment

49
Q

It cause glanders (zoonosis affecting horses, mules, donkeys)

A

B. mallei

50
Q

Can produce several local supportive/acute pulmonary infections.

A

B. mallei

51
Q

It causes melioidosis (an aggresive granulomatous pulmonary disease)

A

B. pseudomallei

52
Q

Pneumonia results from B. pseudomallei resembles/mimic ___ using X-ray

A

Tuberculosis

53
Q

Characteristics of B. pseudomallei colonies and staining.

A

Wrinkled colony and Bipolar staining

54
Q

Selective agar for B. pseudomallei.

A

Ashdown agar

55
Q

Appearance of B. pseudomallei in Ashdown agar.

A

Deep pink colonies with Earthy odor

56
Q

Nonlactose fermenter, which is strongly oxidase (+), nonmotile(Branhamella catarrhalis)

A

Moraxella

57
Q

Similar to moraxella and they are small, paired, gram (-) bacilli and most isolated from urinary tract

A

Oligella

bacte lesson 21 (1 decks)

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