NONFAST, FERM Flashcards
What are the most appropriate screening tests to presumptively differentiate and identify the nonfermentative gram-negative bacilli (NFB) from the Enterobacteriaceae?
A. Catalase, decarboxylation of arginine, growth on blood agar
B. Motility, urease, morphology on blood agar
C. Oxidase, TSI, nitrate reduction, growth on MacConkey agar
D. Oxidase, indole, and growth on blood agar
C. Oxidase, TSI, nitrate reduction, growth on MacConkey agar
Presumptive tests used for identification of the Pseudomonas spp. are:
A. Oxidase, oxidation-fermentation (OF) glucose (open), OF glucose (sealed), motility, pigment production
B. Growth on blood agar plate (BAP) and eosin-methylene blue (EMB) agars, lysine decarboxylation, catalase
C. Growth on MacConkey, EMB, and XLD agars and motility
D. Growth on mannitol salt agar and flagellar stain
A. Oxidase, oxidation-fermentation (OF) glucose (open), OF glucose (sealed), motility, pigment
production
Which tests are most appropriate to differentiate between Pseudomonas aeruginosa and Pseudomonas putida?
A. Oxidase, motility, pyoverdin
B. Oxidase, motility, lactose
C. Oxidase, ONPG, DNase
D. Mannitol, nitrate reduction, growth at 42°C
D. Mannitol, nitrate reduction, growth at 42°C
Which test group best differentiates Acinetobacter baumannii from P. aeruginosa?
A. Oxidase, motility, NO3 reduction
B. MacConkey growth, 37°C growth, catalase
C. Blood agar growth, oxidase, catalase
D. Oxidase, TSI, MacConkey growth
A. Oxidase, motility, NO3 reduction
In addition to motility, which test best differentiates Acinetobacter spp. and Alcaligenes spp.?
A. TSI
B. Oxidase
C. Catalase
D. Flagellar stain
B. Oxidase
The most noted differences between P. aeruginosa and Stenotrophomonas maltophilia are:
A. Oxidase, catalase, and TSI
B. Oxidase, catalase, and ONPG
C. Oxidase, 42°C growth, and polar tuft of flagella
D. Catalase, TSI, and pigment
C. Oxidase, 42°C growth, and polar tuft of flagella
Which Pseudomonas is usually associated with a lung infection related to cystic fibrosis?
A. P. fluorescens
B. P. aeruginosa
C. P. putida
D. Burkholderia pseudomallei
B. P. aeruginosa
A nonfermenter recovered from an eye wound is oxidase positive, motile with polar monotrichous flagella, and grows at 42°C. Colonies are dry, wrinkled or smooth, buff to light brown in color, and are difficult to remove from the agar. In which DNA homology group should this organism be placed?
A. Pseudomonas stutzeri
B. Pseudomonas fluorescens
C. Pseudomonas alcaligenes
D. Pseudomonas diminuta
A. Pseudomonas stutzeri
Which organism is associated with immunodeficiency syndromes and melioidosis (a glanders-like disease in Southeast Asia and northern Australia)?
A. Pseudomonas aeruginosa
B. Pseudomonas stutzeri
C. Pseudomonas putida
D. Burkholderia pseudomallei
D. Burkholderia pseudomallei
Which biochemical tests are needed to differentiate Burkholderia cepacia from S. maltophilia?
A. Pigment on blood agar, oxidase, DNase
B. Pigment on MacConkey agar, flagellar stain, motility
C. Glucose, maltose, lysine decarboxylase
D. TSI, motility, oxidase
A. Pigment on blood agar, oxidase, DNase
The following results were obtained from a pure culture of gram-negative rods recovered from the pulmonary secretions of a 10-year-old cystic fibrosis patient with pneumonia:
Oxidase = +
Glucose OF (open) = +
Pigment = Red (nonfluorescent)
Growth at 42°C = +
Motility = +
Gelatin hydrolysis = +
Arginine dihydrolase = +
Flagella = + (polar, monotrichous)
Which is the most likely organism?
A. Burkholderia pseudomallei
B. Pseudomonas stutzeri
C. Burkholderia cepacia
D. Pseudomonas aeruginosa
D. Pseudomonas aeruginosa
Alcaligenes faecalis (formerly A. odorans) is distinguished from Bordetella bronchiseptica with which test?
A. Urease (rapid)
B. Oxidase
C. Growth on MacConkey agar
D. Motility
A. Urease (rapid)
Chryseobacterium spp. are easily distinguished from Acinetobacter spp. by which of the following two tests?
A. Oxidase, growth on MacConkey agar
B. Oxidase and OF (glucose)
C. TSI and urea hydrolysis
D. TSI and VP
A. Oxidase, growth on MacConkey agar
A gram-negative coccobacillus was recovered on chocolate agar from the CSF of an immunosuppressed patient. The organism
was nonmotile and positive for indophenol oxidase but failed to grow on MacConkey agar. The organism was highly susceptible to penicillin. The most probable identification is:
A. Acinetobacter spp.
B. Pseudomonas aeruginosa
C. Pseudomonas stutzeri
D. Moraxella lacunata
D. Moraxella lacunata
Cetrimide agar is used as a selective isolation agar for which organism?
A. Acinetobacter spp.
B. Pseudomonas aeruginosa
C. Moraxella spp.
D. Stenotrophomonas maltophilia
B. Pseudomonas aeruginosa
A specimen from a 15-year-old female burn patient was cultured after débridement, and the following results were obtained:
Oxidase = +
Catalase = +
Ornithine decarboxylase = Neg
Arginine dihydrolase = +
Lysine decarboxylase = Neg
Motility = +
Glucose = + for oxidation
(open tube)
Maltose = Neg for oxidation
(open tube)
Penicillin = Resistant
Colistin (Polymixin B) = Susceptible
Aminoglycosides = Susceptible
These results indicate which of the following organisms?
A. Acinetobacter baumannii
B. Moraxella lacunata
C. Pseudomonas aeruginosa
D. Acinetobacter lwoffii
C. Pseudomonas aeruginosa
A yellow pigment-producing organism that is oxidase positive, nonmotile, and does not grow on MacConkey agar is:
A. Acinetobacter baumannii
B. Acinetobacter lwoffii
C. Burkholderia cepacia
D. Chryseobacterium meningosepticum
D. Chryseobacterium meningosepticum
Which reagent(s) is (are) used to develop the red color indicative of a positive reaction in the nitrate reduction test?
A. Sulfanilic acid and α-naphthylamine
B. Ehrlich’s and Kovac’s reagents
C. o-Nitrophenyl-β-D-galactopyranoside
D. Kovac’s reagent
A. Sulfanilic acid and α-naphthylamine
A culture from an intra-abdominal abscess produced orange-tan colonies on blood agar that gave the following results:
Oxidase = +
KIA = Alk/Alk (H2S)+
DNase = +
Growth at 42°C = Neg
Nitrate reduction = +
Motility = + (single polar flagellum)
Ornithine decarboxylase = +
The most likely identification is:
A. Shewanella putrefaciens
B. Acinetobacter spp.
C. Pseudomonas aeruginosa
D. Chryseobacterium spp.
A. Shewanella putrefaciens
Chryseobacterium spp. and B. cepacia are easily differentiated by which test?
A. Motility
B. OF glucose
C. Oxidase
D. Cetrimide agar
A. Motility
A 15-year-old female complained of a severe eye irritation after removing her soft-contact lenses. A swab of the infected right eye was obtained by an ophthalmologist, who ordered a culture and sensitivity test. The culture was plated on blood agar and MacConkey agar. At 24 hours, growth of a gram-negative rod that tested positive
for cytochrome oxidase was noted. The Mueller-Hinton sensitivity plate showed a bluish-green “lawn” of growth that proved highly resistant to most of the antibiotics tested except amikacin, tobramycin, and ciprofloxacin. What is the most likely identification?
A. Burkholderia cepacia
B. Pseudomonas aeruginosa
C. Stenotrophomonas maltophilia
D. Acinetobacter baumannii
B. Pseudomonas aeruginosa
Which of the listed Pseudomonas spp. is associated with the following virulence factors: exotoxin A, endotoxins, proteolytic enzymes, antimicrobial resistance, and production of alginate?
A. P. fluorescens
B. P. putida
C. P. stutzeri
D. P. aeruginosa
D. P. aeruginosa
A 20-year-old horse groomer exhibited a “glanders-like” infection. His history indicated he had suffered several open wounds on his hands 2 weeks before the swelling of his lymph nodes. A gram-negative rod was recovered from a blood culture that grew well on blood and MacConkey agars. Most of the biochemical tests were negative, including the cytochrome oxidase test. What is the most likely identification?
A. Burkholderia mallei
B. Pseudomonas aeruginosa
C. Pseudomonas stutzeri
D. Burkholderia pseudomallei
A. Burkholderia mallei
A Vietnam War veteran presented with a “glanders-like” infection (melioidosis). Several blood cultures produced gram-negative rods that were positive for cytochrome oxidase, oxidized glucose and xylose, and grew at 42°C. What is the most likely organism?
A. Stenotrophomonas maltophilia
B. Burkholderia pseudomallei
C. Pseudomonas aeruginosa
D. Acinetobacter spp.
B. Burkholderia pseudomallei