Non-homologous end joining

Question 1

NHEJ takes place when hom seqs do not exist/cannot be found

Answer 1

ya

Question 2

Ku protein binds to DNA ends (but also hp loops, 5’ and 3’ overhangs, and ssDNA)

Answer 2

ya

Question 3

what is the purpose of Ku in NHEJ?

Answer 3

acts as a dock/clamp/scaffold for additional proteins to process the ends

Question 4

step 1 of NHEJ

Answer 4

nucleases bind ku and resect ends–>creating 3’ and 5’ overhangs, nick sealed hps

Question 5

step 2 of NHEJ

Answer 5

polymerase processing–pols that bind Ku fill in the 3’ recessed ends, remove 5’ abasic sugars, and extend off of non-template DNA using terminal transferase activity