Non-Enterobacteriaceae Organisms

Question 1

Pseudomonas species are found in…..

Answer 1

soil, water
hot tubs, whirlpools, sinks, showers
respiratory equipment

Question 2

Psedomonas species are transmitted via

Answer 2

contaminated food, water
medical devices
cosmetics
eye care solution
wounds and person to person

Question 3

Is Pseudomonas aeruginosa normal flora?

Answer 3

No

Question 4

How do most aquire a Pseudomonas aeruginosa infection?

Answer 4

Community or hospital aquired

Question 5

What parts of the body does Pseudomonas aeruginosa infect?

Answer 5

Community: skin (folliculitis), ear (otitis external), respiratory (cystic fibrosis)
Hospital: urinary, bloodstream, respiratory

Question 6

Pseudomonas aeruginoas virulence factos

Answer 6

Endotoxins
Exotoxins
Proteolytic enzymes
Intrinsic Resistance to antimicrobials

Question 7

Psedomonas aeruginosa colonies appear…

Answer 7

Flat and spreading
Green/blue (pyocyanin), red (pyorubrin), brown (pyomelanin)

* Able to grow at 42 degrees*

Question 8

What odor does Pseudomonas aeruginosa have?

Answer 8

Grapelike/ fruity

Question 9

What selective agar is used for Pseudomonas aeruginosa?

Answer 9

Centrimide

Question 10

On blood agar, Pseusomonas aeruginosa will have……

Answer 10

a metallic sheen

Question 11

Vibro species inhabit ______ and are transmitted by ______.

Answer 11

Inhabit fresh or marine water

Transmitted by contaminated water or seafood

Question 12

Vibro secies are gram negative rods which may be _______.

Answer 12

Curved

Question 13

Vibro cholerae causes cholera which has symptoms consisting of…

Answer 13

“rice watery stools”, fluid loss, dehydration, hypotension, an death if untreated

Question 14

Vibro selective media

Answer 14

TCBS

Question 15

Why are vaccines for Vibro cholerae not reccomended for travelers?

Answer 15

The available vaccine offer incomplete protection for a relatively short period of time

Question 16

How are vibro spp. ID’d?

Answer 16

Oxidase positive
Growth in NaCl
API 20E
serotyping

Question 17

Acinetobacter sp. are found in _________, and have no known __________. They are considered _________.

Answer 17

Nature
Virulence factors
opportunistic pathogens

Question 18

What kind of infections do Acinetobacter spp. cause?

Answer 18

Nosocomia infections

UTI, URI, UG tract, wounds, bacteremia

Question 19

Gram stain morphology of Acinetobacter spp.

Answer 19

Gram negative coccobacilli

Question 20

The most common “superbug” isolate of Acinetobacter is

Answer 20

A. colcoaceticus baumanii complex

Question 21

What type of resistance does Acinetobacter have?

Answer 21

Aquired resistance to multiple antimicrobial agents

Question 22

Stenotrophomonas maltophilia is found in

Answer 22

nature and hospitals

Question 23

Stenotrophomonas maltophilia has been isolated from

Answer 23

well water, river water, raw sewage
raw milk, frozen fish
human, rabbit feces

Question 24

Is Stenotrophomoas maltrophilia commonly isolated in the microbiology lab?

Answer 24

Yes

Question 25

What odor is associated with Stenotrophomonas maltrophilia?

Answer 25

Ammonia

Question 26

What type of resistance does Stenotrophomonas maltrophili have?

Answer 26

Intrinsic resistance to multiple antimicrobials

Question 27

Which multiple drug resistant organism infects the military?

Answer 27

MDR Acinetobacter

Question 28

Which organisms have a preference for CO2

Answer 28

Pasturella multocida
Bordetella pertussis
Brucella melitensis
Fransicella tularensis
Eikenella corrodens
Legionella pneumonphilia

Question 29

This organism causes an infection similar to cat-scratch fever. They DO NOT GROWN on MAC.

Answer 29

Pasteurella multocida

Question 30

This organism is isolated from bite wounds and causes SBE

Answer 30

Eikenella corrodens

Question 31

This organism causes Legionnaire’s disease

Answer 31

Legionella pneumophilia

Question 32

Pasteurella multocida gram stiain

Answer 32

ovoid gram negative rods which stain bi-polar(only ends or rods) and is pleomorphic (can change shape in diffrent environmental conditions)

Question 33

Pasturella multocida
On BAP and CHOC colonies will be
On MAC?

Answer 33

gray, non-hemolytic

No growth

Question 34

Pasturella multocida is most frequently recovered from

Answer 34

mouths, respiratory and GI tracts of wild and domestic animals
respiratory tracts of individuals who work with animals

Question 35

Pasturella multocida causes

Answer 35

fowl cholera
opportunistic wound infections
septic arthritis
respiratory infections

Question 36

What organism is common in dog and cat bite or scratch infections?

Answer 36

Pasturella multocida

Question 37

How is Brucella ID’d?

Answer 37

Serum agglutination (SAT)
Dipstick screening
PCR

Question 38

What does Eikenella corrodens typically inhabit?

Answer 38

mucous membranes of humans (mouth and GI tracts)

Question 39

Which organism is the cause of 25% of human hand-bite wound infections and causes subacute endocarditis?

Answer 39

Eikenella corrodens

Question 40

Eikenella corrodens will NOT grow on which agar?

Answer 40

MAC

Question 41

This organism has a bleach odor and causes pitting or corroding of agar.

Answer 41

Eikenella corrodens

Question 42

Legionnaire’s Disease is caused by

Answer 42

Legionella pneumophilia

Question 43

Legionella pneumonphilia is found in

Answer 43

soils and non-marine aqueous environments
pristine waters in tropical rainforest
Industrial water sources (AC, cooling towers, whirpools)
NOT NORMAL FLORA OF HUMANS/ANIMALS

Question 44

Gram stain morphology of Legionella pneumonphilia

Colony appearance

Answer 44

THIN gram negative rods (very faint, not usually seen on gram stain)
Small grey glistening colonies, convex with a “cut glass” appearance

Question 45

BCYE agar is used to culture

Answer 45

Legionella pneumonphilia

Question 46

This organism is an intracellular pathogen

Answer 46

Legionella pneumonphilia