NMD

Question 1

ways to trap rate of decay (3)

Answer 1

1. Stop production, watch (actinomycin D)
2. Transcriptional pulse-chase
3. Approach to steady state - pulse in hot, watch incorporation until you hit steady state

Question 2

suppressor tRNAs

Answer 2

tRNA with an anticodon matching the stop codon, but attaches an amino acid, allowing ribosome to resume translation and readthrough

Question 3

T/F. Most premature stop codons have the same impact on halting protein production.

Answer 3

False, there is a polarity issue

Question 4

what are tethering experiments?

Answer 4

where you incorporate a sequence motif into your RNA of interest that binds a viral protein (MS2), see how those mRNA dynamics are affected by the tethering on N or C terminus

Question 5

how would you find proteins that are involved in frameshifting?

Answer 5

out of frame His4, look for mutants which grow on low His media

Question 6

how does the cell retain memory of introns in mRNA post splicing

Answer 6

exon junction complexes

Question 7

how were RNA ligations made possible?

Answer 7

with a DNA splint and DNA ligase

Question 8

RNase H degrades…

Answer 8

RNA/DNA hybrids

Question 9

where do most UPFs bind?

Answer 9

3’ UTR noncoding

Question 10

what is a pioneer round of translation?

Answer 10

model, the idea is that a preliminary round of translation “clears” the RNA of EJC proteins and other junk, then real productive full-length translation can follow