NGS

Question 1

454 pyrosequencing uses a by-product of the incorporation of NTs into the growing oligonucleotide strand as a substrate to generate detectable light. what is the by-product used?

Answer 1

Pyrophosphate is the by-product of incorporation of nucleotides that together with APS gets converted to ATP and sulphate. the ATP is catalysed by luciferase to produce measurable light