Nematodes Flashcards
Number of nematode species that have been described
200,000
The majority of nematodes are _______ and ______
small predators and saprophytes (eat decomposing organic matter)
provide powerful gene2c models to study the basis of development, aging and many diseases including cancer
Nematodes
Describe the anatomy of nematode
unsegmented: • bilaterally symmetric • triploblas2c: – ectoderm, mesoderm and endoderm.
What does triploblastic mean?
has 3 layers:
ecto, meso and endo DERM
thin layer of
lipids and proteins
Epicuticle
made from crosslinked
cu2cilin proteins
and collagen fibres
cortex
are formed
by collagen fibers which
are put down at angles to
generate a lattice
Basal layers
fibrous and
closest to hypodermis
Basal layer
Nematodes molt how many times?
4x
explain how they molt
The old cu2cle separates from the epidermis and the innermost layer
is par2ally hydrolyzed
• A new cu2cula is secreted by the hypodermis star2ng with the
epicu2cle
• The old cu2cle is shed
Nematodes lack _________
circular
muscles, they depend
entirely on longitudinal
muscles
is generated by the interplay of muscles, pressurized pseudocoel and cuticula
Nematode’s motility
The brain consists of
a single ring around the pharynx. A lateral and a ventral nerve extend back through the length of the worm. Labial and aphid nerves extend forward to enervate sensory organs (amphids and various papilla)
What are amphids?
are simple organs of mechano‐ and chemorecep2on. The sensory organelles are modified cilia
What kind of skeleton do they have?
hydrostatic
is tightly attached to the hypodermis and the cuticle
musculature
What kind of digestive system do they have?
complete but simple.
Mouth is located at the very tip of the animal
is nonmuscular, and consists of a single layer tall columnar cells which carry microvilli and an underlying basal lamina
The intestine
Most nematodes are what in terms of reproduction?
dioecous - two sexes. But, hermaphrodite can occur as well.
Describe the differences between male and female
Males are smaller than females. And they have an additional external features at the posterior end of the worm
Both sexes are attracted to _________
pheromones
is muscular
an sperm is transferred to
the female against the
pseudcoel pressure
The sperm duct
are important
features to tell different
nematodes apart
shape and size of spicules
and bursa
Male inserts ______ ________ copulatory spicules
two sclerotized
This can close vulva in some species.
Anatomy of a male
cement gland
It has no role in sperm motility
actin
The sperm motility depends on
Major Sperm Protein (MSP)
forms dimers which
assemble into subfilaments,
two of these form a helical
filament
MSP
How many ovaries does a female nematode often have?
2
oocytes are fertilized by sperm stored in the ______
receptable
Eutelic means what?
they reach a predefined number of cells which then remains constant (only the germline keeps dividing)
Some cells lose ____ _____ while other proceed to polypoidy.
nuclear DNA
how many larval stages must it have before reaching adulthood?
4 , molting is required each step
Many nematodes can
develop a specialized
resting or ___________
Dauer version of the L3 larval stage
it is the infectious stage for the final host
L3 Larval stage
Larval stages are mostly aerobic or anaerobic?
aerobic
Where do most adults form?
in the intestine because they have little amount of oxygen and have an anaerobic fermentative metabolism.
The third most common roundworm of humans
Trichuris trichuria aka the whip worm
more frequent in areas with poor sanitation and tropical weather
whip worm
Trichuris: adults live here, where they release 20,000 eggs per day.
cecum
Embryonation occurs within ____ _____ in soil
3 weeks
infection is through
ingestion of eggs
Trichuris: Infections with less than 10 worms are asymptomatic or symptomatic?
asymptomatic
Clinical symtoms: Trichuris
abdominal pain cramps weight loss diarrhea (might be bloody) rectal prolapse
Diagnosis : Trichuris
by demonstra2on
of characteris2c eggs
(“champagne corks”)
By procotoscopy
Treatment : trichuris
mebendazole
Prevention: Trichuris
sanitary disposal of feces
Trichinosis is caused by
Trichinella spiralis
life cycle of trichinella
occurs through larvae encysted in muscle tissue.
Larva enters the small intestine and it is freed from its nurse cell.
Larvae grow and molt 4 times
females give birth to live L1 larvae
Larvae enter the blood vessels and carried throughout the body
Females die after how many weeks?
4 to 16 weeks
Both host cell and
worm are enclosed by __________
Collagen capsule
- collagen RNA
symptoms of trichinella.
Remember: this affects MUSCLE
Ac2vity of the females can cause inflammatory reac2on in the mucosa, leading to diarrhea and abdominal pain Characteris2c splinter hemorrhages can be found under finger nails • Fever and chills can persist for weeks • Headache is common and dizziness may develop • Muscle swelling, aching and tenderness occurs often
Father of Pathology
Rudolf Virchow
discovered the life cycle of trichinella
Rudolf Virchow
How is trichinella diagnose?
muscle samples.
ELISA to detect antibody
Large intestinal nematodes
Ascaris lumbricoides
Describe the anatomy of ascaris
Males are 15‐31 cm and have incurved posterior end, females are 20‐49 cm • The anterior end of both sexes shows three lips
Infection of ascaris depends on
fecal contamination of:
soil
food
water
Differentiate fertilized and unfertilized eggs of ascaris
Fertilized eggs are
shorter and rounder
than unfertilized
a mass of knotted worms
obstructing the intes2ne
volvulus
Adult ascaris worms penetrate which organ(s)?
Bile duct and liver
are small nematodes.
Ancylostoma duodenale & Necator
americanus ‐‐ human hookworms
Describe the anatomy of hookworms
Head is slightly bend (hook) and the ‘mouth’ carries characteris2c teeth (Ancylostoma) or plates (Necator, note that these are not real teeth but cu2cular forma2ons of the ‘buccal capsule) • The posterior end of the male worm is elaborated into a copulatory bursa
is found in Europe around the Mediterranean, on the West coast of South America and in parts of China and India
ancylostoma
is found over
much of the western
hemisphere, Africa and
South East Asia
Necator
second most
common human
helminthic infection
hookworm
Adult hookworms live where?
small intestine
how many times do hookworms molt?
twice before maturity
What happens if hookworms come in contact with a host?
If they come into contact with the
host they penetrate the skin, enter
blood vessels and leave the
circulatory system into the alveoli
What is ground itch?
infection by hookworms.
It is the skin penetration and associated with bacterial infection
What is the main concern with hook worm disease?
blood loss
- anemia
- Fe deficiency
- manutrition
Treatment for hookworms
mebendazole