Nematodes Flashcards
what do you call adult female parasite that doesn’t require male parasite to reproduce?
Parthenogenic
what do you call parasites that have male and female reproductive organs?
Dioecious
most common intestinal nematode in man in Philippines
Ascaris lumbricoides
Common name of Ascaris lumbricoides
Giant intestinal roundworm / eelworm
It is the largest nematode
Ascaris lumbricoides
it harbors the sexual or the adult stage of parasite
definitive / Final Host
it harbors the asexual or larval stage of the parasite
Intermediate Host
Final Host of Ascaris lumbricoides
Man
Habitat of Ascaris lumbricoides
Small intestine
Diagnostic stage of Ascaris lumbricoides
Ova and adult
It is the stage that aids the laboratorian to identify the specie of the parasite
Diagnostic stage
It is the stage in the cycle that could infect the host
Infective stage
Infective stage of Ascaris lumbricoides
Ova (embryonated egg)
mode of transmission of Ascaris lumbricoides
Ingestion
These parasites are common to infect child simultaneously. They are also called the “HOLY TRINITY”
“HAT”
Hookworm
Ascaris lumbricoides
Trichuris trichiura
It is the disease that is caused by the larval migration to the lungs
Ascaris pneumonitis
Other term for Ascaris pneumonitis
Loeffler’s pneumonia
Disease with elevated eosinophils and Charcot Leyden crystals
Ascaris pneomonitis
The Ascaris of Dogs
Toxocara canis
Ascaris of Cat
Toxocara cati
Ascaris of pig
Ascaris suum
It is the eosinophil degredation product
Charcot Leyden Crystals
what are Visceral Larva Migrans
Toxocara canis
Toxocara cati
Ascaris suum
It is the term used for group of adult worm
Worm bolus
erratic epigastric pain, Diarrhea, Malnutrition are caused by what stage of Ascaris lumbricoides?
Choices:
Ova
Larva
Adult
Adult
Morphology of Adult Ascaris lumbricoides
creamy white with pink tint
Anterior head: trilobate lips
Term used for having three lips
Trilobate lips
3 layers of ova
- outermost mammillation / albuminoid layer
- middle glycogen layer
- Inner vitelline membrane / lipoidal / lecithin layer
no inner vitelline layer with lecithin granules
Unfertilized Ova
All layers of ova are present
Fertilized corticated ova
No outermost mammillation layer
Fertilized decorticated ova
with embryo inside or presence of larva
Embryonated
What are the parasites found in small intestine?
“CASH”
C. philippinensis
A. lumbricoides
S. stercolaris
Hookworms
What are the parasites found in large intestine?
E. vermicularis
T. trichiura
2 common human hookworm pathogen
N. americanus
A. duodenale
what are the parasites that are able to migrate from intestines to lungs causing pneumonitis?
Larval stages of the ff. parasites
A. lumbricoides
S. stercoralis
Hookworms
elongated, cylindrical anf unsegmented
Adult worm of Nematodes
elongated but segmented
Adult worm of Cestodes
Common name is whipworm
Trichuris trichiura
Final host of Trichuris trichiura
Man
Habitat of Trichuris trichiura
large intestine
Diganostic stage of Trichuris trichiura
Ova
Infective stage Trichuris trichiura
fully embryonated egg
MOD of Trichuris trichiura
Ingestion
Pathology of Trichuris trichiura
Bloody Diarrhea
Epigastric pain
Iron Deficiency Anemia
Rectal prolapse
Hypoalbuminemia
Microscopic, Hypochromic anemia
Iron Deficiency Anemia
It is an edema due to elevated number of adult Trichuris trichiura
Rectal prolapse
Morphology of Trichuris trichiura that is for pin-fashion attachments
Anterior 3/5 is attenuated (from manipis to malaki)
sex of Trichuris trichiura that has curved posterior fleshy portion
male
sex of Trichuris trichiura that has pointed posterior fleshy portion
female
Morphology of Trichuris trichiura ova
it has prominent mucoid bipolar plugs
yellowish brown in color due to bile
What are the shapes of Trichuris trichiura ova?
football
Barrel
Lemon
Japanese lantern
It is a parasite that is termed as “close relative of Trichuris trichiura
Capillaria philippinensis
Who discovered Capillaria philippinensis?
Nelia Salazar
Where did Nelia Salazar found Capillaria philippinensis?
Pudor, Ilocos Sur
Common name of Capillaria philippinensis
Pudoc worm
final host of Capillaria philippinensis
other vertebrae or man
habitat of Capillaria philippinensis
small intestine
Diagnostic stage of Capillaria philippinensis
larva or ova found in STOOL
Infective stage of Capillaria philippinensis
larva or ova found in FISHES
MOT of Capillaria philippinensis
Ingestion of raw or uncooked fish with infected larva
also known as Mystery Disease
Pudoc Disease
gurgling sound of the stomach (Hallmark of Capillariasis)
Borborygmus
Pathology of Capillaria philippinensis
Mystery Disease
Borborygmus
severe diarrhea
abdominal pain
Morphology of Adult Capillaria philippinensis
Delicate worms
Male: with chitinized spicule
Female: usually with eggs in the uterus
Morphology of Capillaria philippinensis ova
with flattened mucoid bipolar plugs
with striated shells
What are the shapes of Capillaria philippinensis ova
guitar
peanut-shaped
What are the natural host of Capillaria philippinensis
Migrating birds (herons, egrets, bitterns)
What are the intermediate hosts of Capillaria philippinensis
Fresh water fishes / brackish water fishes
Glass fishes
Bagtu, bagsang, birot, ipon (Hypseleotris bipartita)
what is the term used for hermaphrodyte that has both female and male reproductive organ?
Monoecious
Nematodes are also known as
Round worms
common nematode in ma (US)
Enterobius vermicularis
Other names of Enterobius vermicularis
Oxyuris vermicularis
Pinworms
seatworms
Society worm
TIWA (Philippines)
The only host of Enterobius vermicularis
Man
Habitat of Enterobius vermicularis
Large intestine
Diagnostic stage of Enterobius vermicularis
Ova, adult
Infective stage
Ova
MOT of Enterobius vermicularis
- Ingestion (most common)
- Inhalation
- Retroinfection - nealy hatched larva migrates back into the host
- Autoinfection - hand-to-mouth transmission (adult female are nocturnals. They migrates to the anal area and lay eggs causing pruritis ani
Pathology of Enterobius vermicularis
- Nocturnal pruritis ani
- Insomia
- Irritation
- Loss of appetite
- Extraintestinal enterobiasis (migration of parasite from anus to vagina, uterus, or fallopian tube)
Morphology of adult Enterobius vermicularis
with cephalic alae (lateral way)
Male: curved posterior end; has flask-shaped or bulb-like esophagus
Female: pointed posterior end; often has gravid uterus filled with eggs
Morphology of Enterobius vermicularis ova
oval shaped, flattened on the side
with double-layered shell
What are the shapes of Enterobius vermicularis ova
loopsided ovum
“D” shaped ovum
Nematodes’ life cycle
- egg
- larva
- adult
3 larval stages
L1 - rhabditiform larva
L2 - rhabditiform larva
L3 - filariform larva
Reproductive organ of parasite is called?
Spicule
Which one is larger? male or female
female
Which one is smaller? male or female
male
It lays immature or unebryonated eggs
oviparous
It lays mature or embryonated eggs
oviviparous or ovoviviparous
don’t lay eggs but lay larva
viviparous or larviparous
Stool examination
- Direct Fecal Smear
- Kato-thick
- Kato-Katz
uses 2 mg of stool + 1 drop of 0.85% NaCl (NSS)
Direct fecal Smear
Routine method of stool examination
Direct fecal Smear
What are the stains used for direct fecal smear?
- lugol’s Iodine
- Nair’s Buffered methylene Blue (BMB)
What is the stain that can’t recover trophozoides of amoeba as they are labile
lugol’s iodine
Direct fecal smear is examined under what microscope objective?
LPO and HPO
What are the reasons why you may get negative result for direct fecal smear?
- No infection
- early infection
- all male infection
Uses 50-60 mg of stool covered with cellophane
Kato-thick
In kato-thick method, it is soaked in the mixture of Glycerin and malachite green solution
Cellophane
Clearing agent used in kato-thick
Glycerin
It gives a pale green color in kato-thick
malachite green
Substitute for malachite green in kato-thick
green cellophane soaked in glycerin
Other term for kato-katz
Cellophane-covered thick smear
Recommended method fro egg counting procedure
Kato-katz
kato-katz uses what other materials aside from glycerin and malachite green?
template and wire mesh
Concentration Techniques
- sedimentation technique
- floatation technique
what are the general principles of concentration techniques
specific gravity and centrifugation
this is the technique where specific gravity of parasite is higher than the reagent
sedimentation technique
2 methods under sedimentation technique
- formalin-ether concentration technique
- acid ether concentration technique
It is the method of choice if specimen is from animal source
acid-ether concentration technique
acid-ether concentration technique is recommended for the recovery of what?
Trichuris trichiura ova, Capillaria philippinensis ova, and Schistosoma ova
It is a technique used if the specific gravity of the parasite is lower than the reagent
floatation technique
a floatation technique that uses 33% zinc sulfate
Zinc sulfate concentration technique
when the specific gravity of stool is 1.18 means what?
It is fresh stool
when the specific gravity of stool is 1.20 means what?
It is fixed stool
recommended method for concentration of oocysts
Sheather’s floatation
not suitable for recovery of operculated ova, hookworm ova (shrink), Schistosoma ova
Brine’s floatation
uses salt solution and no centrifugation required
Brine’s floatation
recommended method for recovery of enterobius vermicularis
Cellulose tape / scotch tape method
Time of collection for Cellulose tape / scotch tape method
Night or early morning
2nd most common helminth to infect man
Hookworms
Common name of Necator americanus
New World Hookworm
American Hookworm
American Murderer
Final Host of Necator americanus
man
Habitat of Necator americanus
Small intestine
Diagnostic stage of Necator americanus
Rhabditiform larva or ova (L1 and L2)
Infective stage of Necator americanus
3rd stage larva (filariform)
MOT of Necator americanus
skin penetration of infective larva
Pathology due to Necator americanus LARVA or ADULT?
Allergic reaction (dermatitis) such as ground itch, dew itch, colic itch, water sore, mazza mora
Larva
Pathology due to Necator americanus LARVA or ADULT?
Pneumonitis
Larva