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BACTE (CHAPTER 17) > Neisseria Spp. > Flashcards

Neisseria Spp. Flashcards

1
Q

Which of the following is/are not a characteristics of Neisseria?
A. Nonmotile
B. Non-spore forming
C. Gram negative diplococci
D. All are catalase (+)

A

D. Not all are (+) some are negative like N.elongata and N. bacilliformis

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2
Q

T or F
Neisseria spp. Are capnophilic.

A

True

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3
Q

What is the natural habitat of Neisseria spp.?

A

Mucous membrane

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4
Q

Other name of N. gonorrhoeae and
N. meningitidis

A

-Gonococci
-Meningococci

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5
Q

They are the primary human pathogens of the Neisseria spp.

A

N. gonorrhoeae
N. meningitidis

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6
Q

T or F
N. gonorrhoeae can be normal flora in the body.

A

False- they are always pathogenic

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7
Q

Neisseria sp that is usually found as a commensal inhabitant of the upper respiratory tract but can also become invasive

A

N. meningitidis

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8
Q

Nesseria spp. are fastidious, and they grow best in what media?

A

Enriched media (CHOC)

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9
Q

Neisseria require ___ in ____for growth.

A

Iron in transferrin.

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10
Q

What are the virulence factors of Neisseria spp?

A
  • Receptors for human transferrin
  • Capsule
  • Pili
  • cell membrane protein
  • lipooligosaccharide or endotoxin
    -IgA protease
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11
Q

Colony type of N. gonorrhoeae that has pili.

A

T1 and T2

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12
Q

Colony type of Neisseria that doesn’t have pil

A

T3 to T5

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13
Q

Neisseria type that is more virulent.

A

T1 and T2

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14
Q

Cell membrane protein that si found in the surface of bacterial cells

A

Por (porin) protein 1

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15
Q

Two type of Por protein of Neisseria.

A

PorA
PorB

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16
Q

Por gene that onlyfound in N. gonorrhoeae

A

PorB

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17
Q

T or F
porA and porB gene are both found in N. meningitidis

A

True

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18
Q

Also known as protein 2

A

Opa (opacity)

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19
Q

Type of cell membrane protein of Neisseria for the adherence to phagocytic and epithelial cell

A

Protein 2 (Opa)

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20
Q

Also known as the Protein 3.

A

Reduction modified protein (Rmp)

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21
Q

This cell membrane protein of Neisseria blocks host serum bactericidal action (IgG)

A

Rmp protein (protein 3)

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22
Q

Major in vivo virulence factors that mediates damage to body tissues and elicits inflammatory response

A

Lipooligosaccharide (LOS)/ Endotoxin

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23
Q

LOS damage tissue by ___.

A

Blebs

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24
Q

Only natural host of N. gonorrhoeae.

A

Human

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25
Q

An acute pyogenic infection of non-ciliated columnar and transitional epithelium

A

Gonorrhea

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26
Q

Primary reservoir of N. gonorrhoeae.

A

Asymptomatic carriers

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27
Q

Gonorrhea in men is usually ___, and in women are ___.

A

Men: Acute urethritis
Women: endocervix

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28
Q

(Men) resulting in purulent discharge and dysuria

A

Acute urethritis

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29
Q

Complication of gonorrhea in men are ___.

A

Prostatitis and epididymitis

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30
Q

(Women) cervical discharge, dysuria, lower abdominal pain.

A

Endocervix

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31
Q

Common complications of gonorrhea in women.

A

Pelvic inflammatory disease (PID)
Fitz-Hugh-Curtis syndrome

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32
Q

Complication of Gonorrhea causes by sterility, ectopic pregnancy or perihepatitis

A

Fitz-Hugh-Curtis syndrome

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33
Q

Blood-borne dissemination of N. gonorrhoeae results in ___.

A

Purulent arthritits

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34
Q

The gonorrhea infection is common in homosexual intercoarse, especially with men.

A

Anorectal of Oropharyngeal infections

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35
Q

Gonorrhea infections tha infect the baby.

A

Opthalmia neonatorum

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36
Q

Specimens for diagnosing Gonorrhea.

A

Genital sites
Rectum
Pharynx
Joint fluid

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37
Q

Specimen of choice for Gonorrhea infection.

A

Men: urethra (pus)
Female: endocervix

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38
Q

Preferred swab for collecting N. gonorrhoeae.

A

Dacron or rayon swabs

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39
Q

T or F
N. gonorrhoeae is resistant to drying and temperature changes.

A

False -susceptible

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40
Q

What are the transport system for N. gonorrhoeae?

A

JEMBEC plates
Gono-pak
Transgrow
Amies with charcoal

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41
Q

The swab containing the N. gonorrhoeae should be rolled in ____.

A

Z pattern.

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42
Q

Shape of N. gonorrhoeae under the microscope

A

Kidney/coffee bean shaped

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43
Q

Thayer-Martin Inhibitory agents.

A

Vancomycin
Colistin
Nystatin

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44
Q

What inhibitory agent was added in Modified Thayer-Martin?

A

Trimethoprim

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45
Q

Modified Thayer-Martin
Vancomycin:
Colistin:
Nystatin:
Trimethoprim:

A

Gram (+)
Gram (-)
Yeast
Swarming proteus soo.

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46
Q

In Martin-Lewis, what are the Inhibitory reagent?

A

Vancomycin
Colistin
Anisomycin
Trimethoprim

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47
Q

In Martin-Lewis what is the inhibitory reagent for yeast?

A

Anisomycin

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48
Q

What do you call the modified Martin-Lewis?

A

New York City

49
Q

What inhibitory agent was modified in New York City?

A

Amphotericin B

50
Q

In New York City medium, what is the inhibitory agent for yeast?

A

Amphotericin B

51
Q

What are the inhibitory agents in GC-LECT medium?

A

Vancomycin
Lincomycin
Colistin
Amphotericin B
Trimethoprim

52
Q

In GC-LECT, what inhibitory agent is for gram (+)?

A

Lincomycin
Vancomycin

53
Q

Inoculated plates of N. gonorrhoeae should be incubated at what temp. And %of CO2?

A

At 35°C
3-5% CO2

54
Q

What is used for incubation of N
gonorrhoeae?

A

Candle extinction jar or CO2 incubator

55
Q

Which among is not the characteristics of N. gonorrhoeae colony morphology?
A. Small
B. Grey-tan
C. Translucent
D. Tenacious
E. Raised

56
Q

Characteristics of T1 and T2 colonies.

A

Small
Raised
Translucent
Brighter
Tan

57
Q

Characteristics of T3-T5 colonies.

A

Large
Flat
Dryer

58
Q

They produce smaller colonies and grow more slowly compare to N. gonorrhoeae.

A

Arginine Hypoxanthine Uracil (AHU)

59
Q

Appearance of N. gonorrhoeae
In oxidase test.

60
Q

Color of colony of N. gonorrhoeae when an oxidizing agent is added directly to the colonies

A

Deep purple -black

61
Q

Traditional method in identifying Neisseria spp.

A

Carbohydrate Utilization

62
Q

In traditional method in identifying the N. gonorrhoeae, what media is used?

A

Cystine trypticase Agar

63
Q

The CTA in Identifying N. gonorrhoeae contains what?

A

1% carbohydrate
Phenol red (pH indicator)

64
Q

N. gonorrhoeae only ferment what CHO?

65
Q

It is used to detect enzymes that hydrolyze colorless substrates and produce colored end products.

A

Chromogenic substrates

66
Q

The advantage of Chromogenic substrates.

A

Identification of strains of N. gonorrhoeae

67
Q

Method employs monoclonal antibodies for identification of N. gonorrhoeae.

A

Immunologic Assays

68
Q

Modification of catalase test (auxotypes).

A

Superoxol (30% H2O2)

69
Q

Most common auxotype

A

AHU strain

70
Q

Antimicrobial resistance of N. gonorrhoeae.

A

PPNG -Plasmid-mediated penicillinase-producing Neisseria gonorrhoeae.
CMRNG- Chromosome-mediated penicillin resistance to both penicillin & tetracycline

71
Q

Treatment for Gonorrhea.

A

Cephalosporins (ceftriaxone & cefixime)

72
Q

N. meningitidis are important etiologic agent of endemic and epidemic of __.

A

Meningitis
Meningococcemia

73
Q

N. meningitidis are normal foud usually in __.

A

Mucous membrane of nasopharynx and oropharynx

74
Q

T or F
por A and por B are both present in N. meningitidis.

75
Q

Route of transmission for N. meningitidis.

A

Oral secretion/Respiratory droplets

76
Q

When N. meningitidis enters the bloodstream, and two main diseases can occur. What are they?

A

Fulminant meningococcemia (deadly)
Meningitis

77
Q

Meningococcemia or sepsis may develop what?

A
  • Purpura with petechial skin rash
  • tachycardia
  • hypotension
    -thrombosis (common)
78
Q

Fulminant meningococcemia can develop what?

A
  • disseminated intravascular coagulation (DIC)
  • septic shock
  • hemorrhage in the adrenal glands (Waterhouse-Friderichsen syndrome)
79
Q

Individual with deficiency of complement components ___ are at risk of of meningococcemia.

80
Q

What serogroup usually cause Meningococcal pneumonia?

A

Serogroup Y

81
Q

What specimens can N. meningitidis recover?

A

CSF
Blood
Nasopharyngeal swabs or aspirate
Joint fluid
Sputum and urugenital sites (less common)

82
Q

Apperance of N. meningitidis under the microscope.

A

Intracellular and Extracellular gram(-) diplococci

83
Q

Media for N. meningitidis.

A

SBA
CHOC (ordinary can be used)

84
Q

Appearance of N. meningitidis in SBA.

A

Bluish gray colonies

85
Q

Appearance of N. meningitidis in CHOC.

A

Tan, small, sometimes mucoid, convex

86
Q

Which is/are not true about N. meningitidis?
A. Catalase (+)
B. Oxidatice (+)
C. Utilize maltose and glucose
D. Utilize fructose and glucose

87
Q

A nonpathogenic that mimics the N. meningitidis.

A

N. lactamica

88
Q

Drug of choice for meningitis.

A

Penicillin

89
Q

Drug of choice for profilaxis for close contact to N. meningitidis.

A

Rifampin
Sulfunamides

90
Q

A polysaccharide-protein conjugated vaccine for N. meningitidis.

A

Menactra vaccine

91
Q

M. catarrhalis belongs to family of ____, which contains 3 genera: ____.

A

Moraxellaceae

Moraxella
Acinetobacter
Psychrobacter

92
Q

They are commensal of upper respiratory tract.

A

M. catarrhalis

93
Q

M. catarrhalis can cause ___, especially in adults with COPD

A

Lower respiratory tract infection

94
Q

Usual URT infection cause by M. catarrhalis.

A

Common cold

95
Q

Identify the common cause of acute otitis media and sinusitis in children in order.

A

1st- S. pneumoniae
2nd- Haemophilus influenzae
3rd- M. catarrhalis

96
Q

Which is/are not true about M. catarrhalis?
A. They grow in SBA and CHOC
B. smooth
C. Shiny
D. Gray to white
E. Convex

97
Q

It has been used to describe the colonies of M. catarrhalis because it remains intact when pushed across the plate with loops.

A

Hockey pucks

98
Q

Appearance of old colonies of M. catarrhalis.

A

Wagon-wheel appearance

99
Q

T or F
M. catarrhalis can grow in high temperature.

A

False- low temperature and grows well at 28°C

100
Q

What sugar does M. catarrhalis ferment?

A

They are ASACCHAROLYTIC (don’t ferment sugar)

101
Q

Which is not true about biochem test for N. gonorrhoeae?
A. DNase (+)
B. Butyrate (-)
C. Butyrate (+)
D. Catalase (+)
E. Oxiase (-)

102
Q

What group of N. Spp. Are traditional pathogens.

103
Q

Group of Neisseria spp. That can grow on selective media.

104
Q

Group of commensal Neisseria spp. That not usually grow in selective media

105
Q

Group 2 and 3 Neissera are subidvided further, what are they?

A

Sacchrolytic
Asacchrolytic

106
Q

They are misidentified as N. gonorrhoeae

A

N. cinerea

107
Q

T or F
N. cinerea is susceptible in Colistin

108
Q

They are yellow pigmented
Asacchrolytic
Grows in SBA and CHOC at 22°C

109
Q

They are found in nasopharynx of children.

A

N. lactamica

110
Q

Misidentified as N. meningitidis.
They are slightly smaller, and they are lactose fermenter.

A

N. Lactamica

111
Q

This N. spp. Are ONPG (+)

A

N. lactamica

112
Q

Large colonies and normal flora of dolphins.

A

N. mucosa.

113
Q

Misidentified as N. Meningitidis
-they release large amount of Extracellular polysaccharide when grows in media that contains 1-5% sucrose.

A

N. polysaccharea

114
Q

The colonies are dry, wrinkled, adherent, and breadcrumbs like.

115
Q

N. spp. able to ferment sucrose amd fructose.

116
Q

Two most common N. spp. Foun in RT of Adult

A

N. sicca
N. Subflava

117
Q

They are less yellow Neisseria sp. And grows at 22°C.

A

N. subflava

118
Q

3 Neisseria spp. That is rod-shaped

A

N. elongata
N. nitroreducens
N. glycolytica

119
Q

Rod shape and normal flora of dogs.

A

N. wequeri

BACTE (CHAPTER 17) (1 decks)

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