NAGE

Question 1

What are the structure of histones?

Answer 1

Histone Octamere : 8 histone proteins, 4 types 2 of each

Question 2

What’s the role of single stranded DNA binding protein ?

Answer 2

make sure DNA is extended

Question 3

Whats the role of the sliding clamp?

Answer 3

Make sure DNA Polymerase is attached to DNA

Question 4

What are the role of primers?

Answer 4

starting point for DNA synthesis. DNA polymerases bind to RNA primer

Question 5

Describe DNA R of e coli

Answer 5

Question 6

What does TFD2 contain?

Answer 6

TBP = tata bidnig protein

TAF = TBP accessory factors

Question 7

What are the sense and anti-sense strands of DNA and state their direction

Answer 7

Sense is coding strand = same as mRNA replaing thymine with uracil 3’–> 5’

Antisense = what mRNA is built from template for trancription 5’–>3’

Question 8

Define promoter region

Answer 8

Regions of DNA which allows trascription to be initiated

TATA sequence and TF binding site

Where BTC assembles

Question 9

what douTF II F binds to RNA Polymerase II bind to ?

Answer 9

TF2 b

Question 10

What is nfkappb aspirin?

Answer 10

NKkappa b TF

aspirin inhibits breakdown Ikb

NFKP reamins in cytoplasm unable to initae transcription of cytokine genes

reduce infalmmation

Question 11

ALL’s and Breast cancerA

Answer 11

Acute lymphoblastic leukemias involev muatted Tf

caused by chromasomal translocations

oestrigen recptor Tf regulate oestrgen genes (also involved in cell growth)

breast canxcer over exprss oestrgen recptor

tamoxifen mimics oestrogen binding to oestrogen receptor –> reducing tumur growth