Myeloid and and Monocytic Maturation Markers Flashcards
1
Q
What is the antigenic profile of
hematopoietic progenetors ?
A
- Positive
- CD34
- CD133
- CD184
- HLA-DR
2
Q
What is the expression pattern of CD13
and CD33 in granulocytic differentiation ?
A
- CD13 and CD33 are expressed at all levels of maturation
- CD13
- brightly expressed by blasts and neutrophils
- moderately expressed by promyelocytes
- dim by metamyelocytes
- CD33
- decreased intensity as cells mature
3
Q
What is the CD45 expression of granulocytes ?
A
- granulocytes have dim expression of CD45
- intensity increases in the final stages of differentiation
- neutrophils have the strongest expression
4
Q
What is the immunophenotype for
Myeloblasts ?
A
- Positive
- CD34
- CD38
- HLA-DR
- CD117- appears after CD34
- CD4
- CD13 (dim) - appears first
- CD33 - follows after CD13
5
Q
What is the immunophenotype of
promyelocytes?
A
- lose CD34 and HLA-DR
- retain CD117
- positive for
- CD13
- CD33 (bright)
- CD4 dim
- start acquiring CD15
- MPO
- CD65
6
Q
What is the immunophenotype of
myelocytes?
A
- CD117 expression is lost
- CD13 expression becomes more dim then gradually increases
- CD11b is acquired- intensity increases to late myelocytes and metamyelocytes
- CD4 is lost during this process
- CD33 expression decreases
- CD15, CD65 positive
- MPO positive
7
Q
What is the immunophenotype of
metamyelocytes ?
A
- start to express CD10 and CD16
- increase in intensity as cells mature to neutrophils
- Segmented forms display bright:
- CD11b, CD11c, CD10, CD16, CD15
- Other markers seen at metamyelocyte/band/segs
- CD24
- CD32
- CD35
- CD65
8
Q
When can you see an upregulation of
CD14 and CD64 expression in granulocytes ?
A
- seen in infections
- patients who are on G-CSF therapy
9
Q
What is the immunophenotype of
a neutrophil ?
A
- CD10 ++
- CD13, CD33
- CD35
- CD11b
- CD15, CD16
- CD65
- MPO
10
Q
What is the immunophenotype of
Basophils ?
A
- very low SSC and dim CD45
- often seen in the blast or hematogone gate
- negative for CD15 and CD16
- dim for CD13
- Positive
- CD9, CD13, CD22, CD25 (dim)
- CD33, CD36, CD38 (bright)
- CD45 (dimmer than lymphocytes)
- CD123 (bright)
- Negative
- CD19, CD34, CD64, CD117, and HLA-DR
11
Q
What is the immunophenotype of
eosinophils ?
A
- very low FSC and very high SSC
- dim CD13 and CD15 (compared to neutrophils)
- negative CD10 and CD16
12
Q
What is the immunophenotype of
Myeloblasts?
A
- Low SSC and variable FSC
- CD4+, CD13+, CD33+
- CD34 +, HLA-DR+
- CD38+, CD117+
- CD123 +/-
- Negative
- CD10, CD11b, CD11c
13
Q
What is the immunophenotype of
Promyelocytes ?
A
- High SSC and high FSC
- CD4+ (dim)
- CD13+, CD33+, CD38+
- CD64+(dim)
- CD117 +
- Negative
- CD11b, CD11c, CD15, CD16
- HLA-DR, CD34
14
Q
What is the immunophenotype of
Myelocytes ?
A
- High SSC and FSC
- CD4 +/- (start to lose it)
- CD11b and CD11c - start to acquire
- CD33+, CD38+
- Negative
- CD10, CD16, CD34
- CD117, HLA-DR
15
Q
What is the immunophenotype of
Metamyelocytes ?
A
- High SSC and High FSC
- CD11b, CD11c
- CD13, CD33
- start to acquire CD10 and CD16
- Negative
- CD4, CD34, HLA-DR
- CD117
