MYCOTIC INFECTIONS ACCORDING TO GEOGRAPHICAL LOCATION: (4) & Other Opportunistic Mycoses Flashcards
Affects the outermost layer of the skin, hand, feet and hair
Superficial Mycoses
- chronic superficial fungal infection which leads to hypopigmented or hyperpigmented patches on skin
- With sunlight exposure the skin around the patches will tan but patches will remain white
Tinea versicolor (an-an) / Pityriasis versicolor
causes tinea or pityriasis versicolor – superficial mycoses; lipophilic yeast; spaghetti with meatball appearance
M. furfur
Mode of transmission of Tinea versicolor (an-an) / Pityriasis versicolor
direct contact
a superficial fungal infection that causes dark brown to black painless patches on the soles of the hands and feet
Tinea nigra
formely called C. werneckii
Exophilia werneckii
- Nodular infection of the hair shaft
- Axillary, pubic, beard, scalp hair maybe infected
Piedra (white and black)
caused by Piedraia hortai
Black Piedra
caused by Trichosporon beigelii/cutaneum
White Piedra
Affects keratinized tissues, skin, hair and nails
Cutaneous Mycoses
Also known as dermatophytosis/dermatomycoses
Cutaneous Mycoses
Also known as Ring worm infection
Cutaneous Mycoses
skin, hair, rarely nails
Microsporum
skin, nails, rarely hair
Trichophyton
skin, hair and nails
Epidermophyton
May be diagnostic of Chromoblastomycosis/Chromomycosis or MOSSY FOOT DISEASE/HEMATOMYCOSIS
Sclerotic bodies / muriform cells
Copper colored fungal cells
Sclerotic bodies / muriform cells
- Unable to produce microconidia
- Macroconidia smooth walled (2-4),
appearing like ____ ___ - Produces ____colored colonies
Epidermorphyton floccosum Eg. Tinea cruris (hadhad)
Khaki; beaver’s tail
Microconidia are globose and arranged in
Grape like clusters
Trichophyton mentagrophytes
- ______ shaped microconidia laterally on
hyphae - May give the so-called “____ __ _ _____”
appearance
Trichophyton rubrum Eg. Tinea corporis
(buni)
Teardrop; Birds on a fence
Produces rat tail or string bean appearing
macroconidia
Trichophyton verrucosum
- Large spindle shaped macroconidia
- Thick-walled spindle shaped large
multiseptate with knobby projections - Rice medium (+) *used to differentiate with
M. audouinii
Microsporum canis Eg. Tinea capitis
- Bizarre shaped macroconidia
- Rice medium (-)
Microsporum audouinii
Type affecting deepest layer of the skin (ie. Muscles)
Subcutaneous Mycoses
- Agents are usually found on soil, so feet is always affected
- Acquired thru trauma
Subcutaneous Mycoses
Sporothrichosis
S. schenckii
usually caused by the following:
Phialophora verrucosa, Cladosporium carrionii; Fonsecaea predosoi
Chromoblastomycosis
caused by the ff: Alternaria, Bipolaris,
Curvularia and Dreschella, Exophialia
Phaeophypomycosis
caused by Pseudoallescheria boydii, Exophiala jeanselmi
Mycetoma
- Causes Rose Gardener’s disease
- Dimorphic fungi
Sporothrix schenckii
- _____ shaped yeast with single or multiple buds
- Conidia in ___________ arrangement
Sporothrix schenckii
Cigar; flowerette
May affect organs such as lungs
Systemic Mycoses
- Caused by dimorphic fungi
- Acquired thru inhalation, so ___ is usually affected
Systemic Mycoses
lungs
Infectious form is ___ (mycelial) form, while diagnostic form is ____
MOLD; YEAST
Produces yeast with single bud on a broad base
Blastomyces dermatitidis
With round or pyriform conidia appearing like lollipops
Blastomyces dermatitidis
Chicago disease
Blastomyces dermatitidis
Gilchrist disease
Blastomyces dermatitidis
Missouri River valley
Blastomyces dermatitidis
- Causes San Joaquin valley fever
- Found in soil of many arid or desert region of US
Coccidioides immitis
Produces a yeast appearing line thick-walled spherule with many endospores
Coccidioides immitis
Barrel shaped arthroconidia alternating with empty dysjuncter cells
Coccidioides immitis
Major biohazard in the lab and most virulent of all the mycotic agents
Coccidioides immitis
On media produces COBWEB appearing colonies
Coccidioides immitis
Virulence factor includes extracellular proteinases
Coccidioides immitis
- Microscopic appearance: Coarse, septate branched hyphae that produce thick
walled barrel shaped rectangular arthroconidia that alternate with dysjuncter cell - 37degC – thick walled spherules filled with endospore with double refractive cell wall
Coccidioides immitis
Microscopic appearance: Mycelia phase – septate hyphae with round or pyriform conidia borne singly in conidiophores or directly on hyphae
Blastomyces dermatitidis
- Produces a yeast within mononuclear cells
- 37degC – small budding yeast within mononuclear cell
- RT – knobby tuberculate will form
Histoplasma capsulatum
To demonstrate yeast, Giemsa or Wright’s may be used
Histoplasma capsulatum
Darling’s disease
Histoplasma capsulatum
Cave disease
Histoplasma capsulatum
Spelunker disease
Histoplasma capsulatum
Reticuloendothelial cytomycosis
Histoplasma capsulatum
Ohio valley fever
Histoplasma capsulatum
Found on bat and bird feces
Histoplasma capsulatum
South American Blastomycosis
Paracoccidioides brasiliensis
Luts Splentdore Almeide disease
Paracoccidioides brasiliensis
37degC - Produces a yeast with multiple buds giving it a Ship’s wheel or mariner’s wheel or Pilot’s wheel appearance. Yeast may resemble a mickey mouse cap
Paracoccidioides brasiliensis
Found in soil and plant; virulent factor include estrogen binding protein
Paracoccidioides brasiliensis
RT: Produces terminally locate chlamydoconidia
Paracoccidioides brasiliensis
Microscopic appearance: Presence of terminal chlamydoconidia mistaken as B. dermatitidis
Paracoccidioides brasiliensis
Major cause of opportunistic infection in those with AIDS
Cryptococcus neoformans
Causes European blastomycosis, Torulosis, Cryptococcosis, Torula Meningitis, Bussebuschke disease
Cryptococcus neoformans
Produces an encapsulated yeast that can be demonstrated using India ink
Cryptococcus neoformans
Phenol oxidase and urease test (+) ; nitrate (-)
Cryptococcus neoformans
- Most common cause of yeast infection
- (+) growth at 42degC
Candida albicans
A normal flora but can be opportunistic
Candida albicans
Causes vulvovaginitis, oral thrush
Candida albicans
Forms Germ tubes, blastoconidia and chlamydospores
Candida albicans
- Causes pulmonary disease, otomycosis
- Acquired thru inhalation
Aspergillus spp
Agent of Fungus ball, Aspergilloma, Farmer’s lung disease
Aspergillus spp.
black pepper effect on culture media, fungus ball in lungs
Aspergillus niger
___most common cause of pulmonary
aspergillosis often found in compost piles and soil of potted plants. ____ color on culture media
Aspergillus fumigatus; Green
(previous called Penicillium marnefei)
Talaramyces marneffei
- Causes Talaromycosis
- Dimorphic fungi
Talaramyces marneffei
Yeast = ____ shaped while mold with ____ shaped phialides, paint brush like TB Symptoms in HIV px in SEA
sausage; flask
- Includes the ff: Genera
o Rhizopus
o Absidia
o Mucos - Causes: Rhinosinus, sinus orbital or craniofacial zygomycosis
Zygomycota (Zygomycetes)
(+) growth on SDA without cycloheximide
Zygomycota (Zygomycetes)
Rapid growers; “___” with ASEPTATE hyphae
Lid lifters
classified as fungi imperfecti, normal mycoflora of commodities (rice)
Fusarium species (a hyaline mold)
wound infection and oral thrush – forms Hockey stick shaped arthroconidia on corn meal agar
Geotrichum
can cause keratitis, external ear infection
Penicillium (hyaline mold)
can cause mycetoma, corneal and nail infections
Acremonium
Previously a protozoa but now an atypical fungi
Pneumocystis jirovecii or carinii
Formerly: Pneumocystis pneumonia (PCP)
Pneumocystis jirovecii or carinii
Pneumonia in AIDS individual acquired from immunocompetent mammals
Pneumocystis jirovecii or carinii
Preferred sample in detecting Pneumocystis jirovecii or carinii
BAL (broncho alveolar lavage)
stain of choice for cysts but not trophozoite
Gomori methenamine silver