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Mycology > Mycology > Flashcards

Mycology Flashcards

1
Q

Fungi are EUKARYOTIC OR PROKARYOTIC

A

Eukaryotic

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2
Q

What fungi lacks, that can be seen in plants?

A

Chlorophyll

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3
Q

Fungi are AEROBIC OR ANAEROBIC

A

AEROBIC

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4
Q

Gram staining of Fungi

A

Gram-positive

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5
Q

Component that can be seen at the cell wall of fungi

A

CHITIN

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6
Q

Yeast cell structure

A

Unicellular

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7
Q

Colonies formed by yeast has what appearance

A

Moist, creamy, opague, pasty

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8
Q

Colonies of yeast best grow at what temperature

A

37 (body temperature

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9
Q

Yeast may appear as YEAST ONLY OR MOLD ONLY, what characteristic is this called

A

MONOMORPHIC

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10
Q

Molds cellular structure

A

Multicellular

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11
Q

Colonies of molds appears

A

Wooly, cottony, fluffy, powdery

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12
Q

Molds best grow at what temperature

A

25-30 (Room temperature)

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13
Q

Molds can appear as both yeast and mold, for short, molds are?

A

DIMORPHIC

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14
Q

Subcutaneous Mycoses

A

Sporothrix schenkii

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15
Q

SYSTEMIC MYCOSES ARE

A

HBCPP
Histoplasma capsulatum
Blastomyces dermititidis
Coccidoides immitis
Paracoccidoides braziliense
Penicillium marneffei

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16
Q

Basic unit structure of FILAMENTOUS FUNGI

A

Hyphae

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17
Q

Septated fungi

A

With cross walls (common)

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18
Q

Sparsely aseptated, aseptated

A

Without cross walls

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19
Q

ZYGOMYCETES ARE? (Based on presence of septate)

A

Aseptate

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20
Q

With spiral hyphae

A

Trichophyton mentagrophytes

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21
Q

With antler hyphae

A

Trichophyton schoenleinii (swollen)

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22
Q

With pectinate body

A

Microsporum audouinii

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23
Q

Racquet hyphae

A

Epidermophyton flocossum (club-shaped)

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24
Q

With nodular bodies

A

Microsporum canis

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25
Q

Non-pigmented/lightly pigmented

A

Hyaline

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26
Q

Pigmented fungi are called

A

Dematiaceous

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27
Q

Pigmentation is due to the presence of

A

MELANIN

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28
Q

Structure for REPRODUCTION

A

SPORES

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29
Q

Asexual spores are also called as

A

Anamorph

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30
Q

Used under temporary mount, and to demonstrate fungal elements in HAIR, SKIN, NAILS

A

10-20% KOH

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31
Q

For preservative and stain in temporary mount

A

Lactophenol cotton blue

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32
Q

Demonstrate C.neoformans

A

Indian Ink/Negrosin

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33
Q

Best used in direct microscopy/Chitin wall

A

Calcouflour White stain

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34
Q

Best used in permanent staining

A

Gomori’s methenamine silver

35
Q

For hyphal pigmentation

A

Fontana Masson and H & E

36
Q

Permanent stain; Detection of C.neoformans

A

Maver’s Mucicarmine

37
Q

Detection of yeast within Mononuclear cell

A

Giemsa or Wrights stain

38
Q

Fungi detected using Wright stain

A

Histoplasma capsulatum

39
Q

Incubation properties of Fungi

A

25-30 (room temperature)
Ambient air
2 weeks (longer)

40
Q

Antimicrobial agent that prevents bacteria overgrowth

A

Chloramphenicol

41
Q

Prevents growth saprophytic fungi

A

Cyclohexamide

42
Q

Presumptive test for C.albicans

A

Germ Tube test (no constriction)

43
Q

With no true constriction

A

Pseudohyphae

44
Q

Test that differentiates T.mentagrophytes from T.rubrum

A

Urease test (+) Pink - red color
Hair baiting test/ Hair perforation test ; (+) Conical shape; V shape

45
Q

Czapek Agar

A

Aspergillus

46
Q

Cornmeal Agar

A

C.albicans

47
Q

Bird seed, Niger, staibs medium

A

C. Neoformans

48
Q

Mycoses, Mycobiotic

A

Dermatophytes

49
Q

Rapid identification for C.neoformans

A

L-dopa ferric citrate test

50
Q

Pytiriasis versicolor causes

A

Malassezia furfur (tinea and Pytyriasis)

51
Q

Versicolor has what kind of spore appearance

A

Spaghetti with meatballs (lipophylic yeast)

52
Q

Pytyriasis versicolor characteristics

A

Hypopigmentation, hyperpigmentation
- Around sun (skin around patches-tan) (Patches-white)

53
Q

Tinea nigra causes

A

Dark brown to black painless patches on skin

54
Q

Fungi agent responsible for Tinea nigra

A

Exophiala werneckii
Cortea werneckii

55
Q

Piedra causes

A

Nodular infection in hair shaft, axillary, beard, pubic and scalp

56
Q

Black Piedra

A

Piedraia hortae

57
Q

White piedra

A

Trichosporon beigelli/cutaneum

58
Q

Contains khaki colored colonies

A

E.floccosum

59
Q

Appearance of macroconidia formed by E.floccosum

A

Smooth walled, with beavers tail appearance

60
Q

E.floccosum causes

A

Tinea cruris

61
Q

Microconidia appears globose, grape like clusters

A

Trichophyton mentagrophytes

62
Q

Teardrop shaped microconidia

A

T.rubrum

63
Q

T.rubrum causes

A

Tinea corporis

64
Q

Other than teardrop shaped appearance of T.rubrum is..

A

Birds-on-a-fence

65
Q

Mycosis that affects deep layer of skin

A

Subcutaneous Mycosis

66
Q

Diseases associated with Subcutaneous mycoses

A

Sporothrycosis
Phaeohypomycosis
Mycetoma

67
Q

Sprothrix schenkii characteristics

A

Cigar shaped yeast
Conidia flowerette arrangement
Rose Gardener’s disease

68
Q

Muriform cells/sclerotic bodies

A

Copper colored fungi
Chromoblastomycosis, chromomycosis, Moss feet disease, hematomycosis

69
Q

Diseases caused by Blastomyces dermatitidis

A

Blastomycosis
North American Blastomycosis
Chicago’s disease
Gillchrist disease
Missouri River Valley

70
Q

Conidia appearance of B.dermatitidis

A

Round or pyriform conidia

71
Q

B.dermatitidis; yeast appearance

A

Single bud w/ broad base

72
Q

Mycelial phase of B.dermatitidis

A

Septate hyphae with pyriform/round conidia attached directly on hyphae or conidiosphores

73
Q

Disease that is caused by HISTOPLASMA CAPSULATUM

A

Cave’s disease
Darling’s disease
Spelunker’s disease
Histoplasmosis
Reticuloendothelial cytomycosis
Ohio vallley fever

74
Q

Histoplasma capsulatum is found in organisms like

A

Bats and birds

75
Q

Stain used in Histoplasma Capsulatum

A

Giemsa/Wright’s stain

76
Q

Mycelial phase of H.capsulatum

A

Septate hyphae with round/pyriform microconidia on short brances of hyphal stalk —> Macrocondia with large knobby projections

77
Q

Virulence factor of H.capsulatum

A

Intracellular growth
CBP for binding calcium
Thermobalance

78
Q

Disease caused by COCCIDOIDES IMMITIS

A

SAN JOAQUIN VALLEY FEVER

79
Q

Yeast appearance of C. Immitis

A

Thick walled appearance, with many endospores

80
Q

Conidia Appearance of C. Immitis

A

Barrel shaped rectangular arthroconidia with empty disjuncter cell

81
Q

Virulence factor of C. Immitis

A

Extracellular growth

82
Q

Mycelial Phase of C. Immitis

A

Coarse, septate, branched hyphae with barrel shaped rectangular arthroconidia with empty dysjuncter cells = uneven staining

83
Q

MOST BIOHAZARDOUS AND VIRULENT IN LAB

A

C. Immitis

Mycology (2 decks)

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