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Mycology Flashcards

1
Q

Fungi are EUKARYOTIC OR PROKARYOTIC

A

Eukaryotic

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2
Q

What fungi lacks, that can be seen in plants?

A

Chlorophyll

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3
Q

Fungi are AEROBIC OR ANAEROBIC

A

AEROBIC

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4
Q

Gram staining of Fungi

A

Gram-positive

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5
Q

Component that can be seen at the cell wall of fungi

A

CHITIN

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6
Q

Yeast cell structure

A

Unicellular

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7
Q

Colonies formed by yeast has what appearance

A

Moist, creamy, opague, pasty

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8
Q

Colonies of yeast best grow at what temperature

A

37 (body temperature

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9
Q

Yeast may appear as YEAST ONLY OR MOLD ONLY, what characteristic is this called

A

MONOMORPHIC

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10
Q

Molds cellular structure

A

Multicellular

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11
Q

Colonies of molds appears

A

Wooly, cottony, fluffy, powdery

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12
Q

Molds best grow at what temperature

A

25-30 (Room temperature)

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13
Q

Molds can appear as both yeast and mold, for short, molds are?

A

DIMORPHIC

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14
Q

Subcutaneous Mycoses

A

Sporothrix schenkii

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15
Q

SYSTEMIC MYCOSES ARE

A

HBCPP
Histoplasma capsulatum
Blastomyces dermititidis
Coccidoides immitis
Paracoccidoides braziliense
Penicillium marneffei

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16
Q

Basic unit structure of FILAMENTOUS FUNGI

A

Hyphae

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17
Q

Septated fungi

A

With cross walls (common)

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18
Q

Sparsely aseptated, aseptated

A

Without cross walls

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19
Q

ZYGOMYCETES ARE? (Based on presence of septate)

A

Aseptate

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20
Q

With spiral hyphae

A

Trichophyton mentagrophytes

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21
Q

With antler hyphae

A

Trichophyton schoenleinii (swollen)

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22
Q

With pectinate body

A

Microsporum audouinii

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23
Q

Racquet hyphae

A

Epidermophyton flocossum (club-shaped)

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24
Q

With nodular bodies

A

Microsporum canis

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25
Non-pigmented/lightly pigmented
Hyaline
26
Pigmented fungi are called
Dematiaceous
27
Pigmentation is due to the presence of
MELANIN
28
Structure for REPRODUCTION
SPORES
29
Asexual spores are also called as
Anamorph
30
Used under temporary mount, and to demonstrate fungal elements in HAIR, SKIN, NAILS
10-20% KOH
31
For preservative and stain in temporary mount
Lactophenol cotton blue
32
Demonstrate C.neoformans
Indian Ink/Negrosin
33
Best used in direct microscopy/Chitin wall
Calcouflour White stain
34
Best used in permanent staining
Gomori’s methenamine silver
35
For hyphal pigmentation
Fontana Masson and H & E
36
Permanent stain; Detection of C.neoformans
Maver’s Mucicarmine
37
Detection of yeast within Mononuclear cell
Giemsa or Wrights stain
38
Fungi detected using Wright stain
Histoplasma capsulatum
39
Incubation properties of Fungi
25-30 (room temperature) Ambient air 2 weeks (longer)
40
Antimicrobial agent that prevents bacteria overgrowth
Chloramphenicol
41
Prevents growth saprophytic fungi
Cyclohexamide
42
Presumptive test for C.albicans
Germ Tube test (no constriction)
43
With no true constriction
Pseudohyphae
44
Test that differentiates T.mentagrophytes from T.rubrum
Urease test (+) Pink - red color Hair baiting test/ Hair perforation test ; (+) Conical shape; V shape
45
Czapek Agar
Aspergillus
46
Cornmeal Agar
C.albicans
47
Bird seed, Niger, staibs medium
C. Neoformans
48
Mycoses, Mycobiotic
Dermatophytes
49
Rapid identification for C.neoformans
L-dopa ferric citrate test
50
Pytiriasis versicolor causes
Malassezia furfur (tinea and Pytyriasis)
51
Versicolor has what kind of spore appearance
Spaghetti with meatballs (lipophylic yeast)
52
Pytyriasis versicolor characteristics
Hypopigmentation, hyperpigmentation - Around sun (skin around patches-tan) (Patches-white)
53
Tinea nigra causes
Dark brown to black painless patches on skin
54
Fungi agent responsible for Tinea nigra
Exophiala werneckii Cortea werneckii
55
Piedra causes
Nodular infection in hair shaft, axillary, beard, pubic and scalp
56
Black Piedra
Piedraia hortae
57
White piedra
Trichosporon beigelli/cutaneum
58
Contains khaki colored colonies
E.floccosum
59
Appearance of macroconidia formed by E.floccosum
Smooth walled, with beavers tail appearance
60
E.floccosum causes
Tinea cruris
61
Microconidia appears globose, grape like clusters
Trichophyton mentagrophytes
62
Teardrop shaped microconidia
T.rubrum
63
T.rubrum causes
Tinea corporis
64
Other than teardrop shaped appearance of T.rubrum is..
Birds-on-a-fence
65
Mycosis that affects deep layer of skin
Subcutaneous Mycosis
66
Diseases associated with Subcutaneous mycoses
Sporothrycosis Phaeohypomycosis Mycetoma
67
Sprothrix schenkii characteristics
Cigar shaped yeast Conidia flowerette arrangement Rose Gardener’s disease
68
Muriform cells/sclerotic bodies
Copper colored fungi Chromoblastomycosis, chromomycosis, Moss feet disease, hematomycosis
69
Diseases caused by Blastomyces dermatitidis
Blastomycosis North American Blastomycosis Chicago’s disease Gillchrist disease Missouri River Valley
70
Conidia appearance of B.dermatitidis
Round or pyriform conidia
71
B.dermatitidis; yeast appearance
Single bud w/ broad base
72
Mycelial phase of B.dermatitidis
Septate hyphae with pyriform/round conidia attached directly on hyphae or conidiosphores
73
Disease that is caused by HISTOPLASMA CAPSULATUM
Cave’s disease Darling’s disease Spelunker’s disease Histoplasmosis Reticuloendothelial cytomycosis Ohio vallley fever
74
Histoplasma capsulatum is found in organisms like
Bats and birds
75
Stain used in Histoplasma Capsulatum
Giemsa/Wright’s stain
76
Mycelial phase of H.capsulatum
Septate hyphae with round/pyriform microconidia on short brances of hyphal stalk —> Macrocondia with large knobby projections
77
Virulence factor of H.capsulatum
Intracellular growth CBP for binding calcium Thermobalance
78
Disease caused by COCCIDOIDES IMMITIS
SAN JOAQUIN VALLEY FEVER
79
Yeast appearance of C. Immitis
Thick walled appearance, with many endospores
80
Conidia Appearance of C. Immitis
Barrel shaped rectangular arthroconidia with empty disjuncter cell
81
Virulence factor of C. Immitis
Extracellular growth
82
Mycelial Phase of C. Immitis
Coarse, septate, branched hyphae with barrel shaped rectangular arthroconidia with empty dysjuncter cells = uneven staining
83
MOST BIOHAZARDOUS AND VIRULENT IN LAB
C. Immitis

Mycology (2 decks)

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