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Mycobacteria Flashcards

1
Q

Not pigmented unless exposed to light optimally during their early growth

A

Photochromagen

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2
Q

Pigmented when grown in the dark or light

A

Scotochromagen

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3
Q

Not pigmented when grown in the dark or in light

A

Nonchromagen

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4
Q

Nontuberculous mycobacteria pigment

A

Runyon’s classification

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5
Q

M.kansassi

A

Photochromagen

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6
Q

M.xenopi

A

Scotochromagen

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7
Q

Battey Bacillus
 In AIDS patients

A

MYCOBACTERIUM AVIUM COMPLEX

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8
Q

“yellow bacillus,

A

MYCOBACTERIUM KANSASII

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9
Q

growth requirement for hemoglobinor hemin

A

MYCOBACTERIUM HAEMOPHILUM

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10
Q

Bairnsdale ulcer

A

MYCOBACTERIUM ULCERANS

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11
Q

disseminated disease in HIV-infected patients

A

MYCOBACTERIUM GENAVENSE

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12
Q

chronic granulomatous infections involving skin and soft tissue, often called”swimming pool granuloma” or “fish tank granuloma”

A

MYCOBACTERIUM MARINUM

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13
Q

Tap-water bacillus

A

MYCOBACTERIUM GORDONAE

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14
Q

etiologic agent of leprosy, also called Hansen’s disease

A

MYCOBACTERIUM LEPRAE

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15
Q

not culturable; unique manifestation in a way that they infect the nerves instead the cells

A

MYCOBACTERIUM LEPRAE

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16
Q

Decontaminating agent of MYCOBACTERIUM LEPRAE

A

N acetyl-L-cysteine(NALC)

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17
Q

digesting agent MYCOBACTERIUM LEPRAE

A

2-4% NaOH

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18
Q

MYCOBACTERIUM TUBERCULOSIS measurement

A

0.2-0.6 micron in diameter and 1-4 micra in length

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19
Q
A
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20
Q

Highly resistant to drying (protected from sunlight)

A

MYCOBACTERIUM TUBERCULOSIS

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21
Q

Duration of MTB in Dried sputum

A

6-8 months

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21
Q

Droplets of dried sputum in the air -

A

8-10 days

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22
Q

Exposed to direct sunlight, MTB for culture are killed

A

within 2 hours

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23
Q

Span of MTB In sputum -

A

20-30 hours

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24
Disinfection of sputum -
24 hours in 5% phenol
25
a disseminated infection with multiple organ involvement.
Miliary TB
26
is miliary tuberculosis in the bones or spine
Pott's disease
27
used to detect MTB-infected individuals
Tuberculin skin test
28
MTB antigen.
purified protein derivative (PPD)
29
Contains 2% glycerol, which enhances the growth of Mycobacterium avium complex (MAC)
Middlebrook
30
31
Supplemented with carbenicillin (for inhibition of pseudomonads), polymyxin B, trimethoprim lactate, and amphotericin B
Middlebrook 7H10
31
Contains 0.1% enzymatic hydrolysate of casein, which improves recovery of isoniazid-resistant Mycobacterium tuberculosis
Middlebrook 7H11
31
Supplemented with mycobactin J, which provides for growth of Mycobacterium genovense
Middlebrook 7H11
32
Commonly used medium; good recovery of M. tuberculosis but poor recovery of many other species; M. genovense fails to grow
Löwenstein Jensen (L-J)
33
Supplemented with penicillin and nalidixic acid
L-J Gruft
34
Supplemented with cycloheximide, lincomycin, and nalidixic acid
L-J Mycobactosel
35
Contains twice the concentration of malachite than LöwensteinJensen green
Petragnani medium
36
Supplemented with hemin, hemoglobin, or ferric ammonium citrate; increases recovery of Mycobacterium haemophilum
Heme-supplemented media (egg or agar based)
37
liquid media duration
10 days
38
solid media duration
17 days
39
liquid media that is used as the standard for Isolating mycobacteria
LJ agar
40
rough, buffered colonies that appeared within 3 weeks
M.tuberculosis in LJ agar
41
Colonies appear as cream-colored, dry, and wrinkled.
M. tuberculosis on Middlebrook 7H11 agar
42
a widely used medium for the isolation and antimicrobial susceptibility testing of mycobacteria
Middlebrook 7H11 agar
43
contains casein hydrolysates that improve the recoveryof isoniazid-resistant strains of M. tuberculosis and shortens the incubation time for M. avium complex isolates.
Middlebrook 7H11 agar
44
colonies have a rough appearance and exhibit serpentine cording,exemplified by the darker areas.
M. tuberculosis
45
have the ability to growat 45°C.
M. avium
46
does not grow well on LJ agar. It appears as a film after 3 to 4 weeks of incubation
M. avium
47
appear as a "sun-spot" with starlike or asteroid margins a
M. avium
48
rough colony has a "lacy" characteristic
M. avium
49
have a strong yellow color; a positive catalase reaction, reduction of nitrate to nitrite, and rapid hydrolysis of Tween 80
M. kansasii on Middlebrook 7H11 agar.
50
3-week-old colony growing on Middlebrook 7H11agar shows a dark, dense center and a wrinkled periphery,
Colony of M. kansasii
51
does not reduce nitrates or assimilate iron, but it is suceptible to polymyxin B and resistant to ciprofloxacin.
M. chelonae
52
grow on chocolate agar and appear as smooth. opaque colonies resembling staphylococci or yeast.
M. chelonae
53
may appear as weakly staining. beaded, gram-positive bacilli, suggesting the possibility of a rapidly growing mycobacterium.
M. chelonae
54
colonies appear dark and dense with smooth edges and a somewhat lighter center
M. chelonae
55
can also grow on modifiedMac-Conkey agar, without crystal violet, at 37°C;
M. fortuitum
56
Optimum temperature for M. fortuitum cultured in 7H11 & LJ
43 deg. Celsius
57
a Runyon group II scotochromogens, characterized by yellow-orane pigment
M. gordonae
58
Classify the Runyon group: M.smegmatis
Rapid growers
59
Classify the Runyon group: M.thermoresistible
Scotochromogens
60
Classify the Runyon group: M.terrae-triviale
Non-photochromogens
61
Reagent strip impregnated with substrate cyanogen halide = yellow color
Niacin accumulation tests
62
Base on the ability to convert ferric ammonium citrate to an iron oxide
Iron uptake test
63
Growth at 28°C on an egg based medium containing 5% NaCl
NaCl tolerance test
64
The only mycobacteria that is negative to NaCl Tolerance test
M.chelonae
65
positive fot

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