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Bacte > Mycobacteria > Flashcards

Mycobacteria Flashcards

1
Q

Not pigmented unless exposed to light optimally during their early growth

A

Photochromagen

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2
Q

Pigmented when grown in the dark or light

A

Scotochromagen

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3
Q

Not pigmented when grown in the dark or in light

A

Nonchromagen

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4
Q

Nontuberculous mycobacteria pigment

A

Runyon’s classification

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5
Q

M.kansassi

A

Photochromagen

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6
Q

M.xenopi

A

Scotochromagen

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7
Q

Battey Bacillus
 In AIDS patients

A

MYCOBACTERIUM AVIUM COMPLEX

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8
Q

“yellow bacillus,

A

MYCOBACTERIUM KANSASII

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9
Q

growth requirement for hemoglobinor hemin

A

MYCOBACTERIUM HAEMOPHILUM

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10
Q

Bairnsdale ulcer

A

MYCOBACTERIUM ULCERANS

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11
Q

disseminated disease in HIV-infected patients

A

MYCOBACTERIUM GENAVENSE

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12
Q

chronic granulomatous infections involving skin and soft tissue, often called”swimming pool granuloma” or “fish tank granuloma”

A

MYCOBACTERIUM MARINUM

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13
Q

Tap-water bacillus

A

MYCOBACTERIUM GORDONAE

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14
Q

etiologic agent of leprosy, also called Hansen’s disease

A

MYCOBACTERIUM LEPRAE

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15
Q

not culturable; unique manifestation in a way that they infect the nerves instead the cells

A

MYCOBACTERIUM LEPRAE

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16
Q

Decontaminating agent of MYCOBACTERIUM LEPRAE

A

N acetyl-L-cysteine(NALC)

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17
Q

digesting agent MYCOBACTERIUM LEPRAE

A

2-4% NaOH

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18
Q

MYCOBACTERIUM TUBERCULOSIS measurement

A

0.2-0.6 micron in diameter and 1-4 micra in length

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19
Q
A
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20
Q

Highly resistant to drying (protected from sunlight)

A

MYCOBACTERIUM TUBERCULOSIS

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21
Q

Duration of MTB in Dried sputum

A

6-8 months

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21
Q

Droplets of dried sputum in the air -

A

8-10 days

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22
Q

Exposed to direct sunlight, MTB for culture are killed

A

within 2 hours

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23
Q

Span of MTB In sputum -

A

20-30 hours

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24
Q

Disinfection of sputum -

A

24 hours in 5% phenol

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25
Q

a disseminated infection with multiple organ
involvement.

A

Miliary TB

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26
Q

is miliary tuberculosis in the bones or spine

A

Pott’s disease

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27
Q

used to detect MTB-infected individuals

A

Tuberculin skin test

28
Q

MTB antigen.

A

purified protein derivative (PPD)

29
Q

Contains 2% glycerol, which enhances the growth of Mycobacterium avium complex (MAC)

A

Middlebrook

30
Q
A
31
Q

Supplemented with carbenicillin
(for inhibition of pseudomonads),
polymyxin B, trimethoprim
lactate, and amphotericin B

A

Middlebrook 7H10

31
Q

Contains 0.1% enzymatic hydrolysate of casein, which improves recovery of isoniazid-resistant Mycobacterium tuberculosis

A

Middlebrook 7H11

31
Q

Supplemented with mycobactin J, which provides for growth of
Mycobacterium genovense

A

Middlebrook 7H11

32
Q

Commonly used medium; good
recovery of M. tuberculosis
but poor recovery of many other species; M. genovense fails to grow

A

Löwenstein Jensen (L-J)

33
Q

Supplemented with penicillin and nalidixic acid

A

L-J Gruft

34
Q

Supplemented with cycloheximide,
lincomycin, and nalidixic acid

A

L-J Mycobactosel

35
Q

Contains twice the concentration
of malachite than LöwensteinJensen green

A

Petragnani medium

36
Q

Supplemented with hemin, hemoglobin, or ferric ammonium citrate; increases recovery of
Mycobacterium haemophilum

A

Heme-supplemented
media (egg or agar
based)

37
Q

liquid media duration

A

10 days

38
Q

solid media duration

A

17 days

39
Q

liquid media that is used as the standard for Isolating mycobacteria

A

LJ agar

40
Q

rough, buffered colonies that appeared within 3 weeks

A

M.tuberculosis in LJ agar

41
Q

Colonies appear as cream-colored, dry, and wrinkled.

A

M. tuberculosis on Middlebrook 7H11 agar

42
Q

a widely used medium for the isolation and antimicrobial susceptibility testing of mycobacteria

A

Middlebrook 7H11 agar

43
Q

contains casein hydrolysates that improve the recoveryof isoniazid-resistant strains of M. tuberculosis and shortens the incubation time for M. avium complex isolates.

A

Middlebrook 7H11 agar

44
Q

colonies have a rough appearance and exhibit serpentine cording,exemplified by the darker areas.

A

M. tuberculosis

45
Q

have the ability to growat 45°C.

A

M. avium

46
Q

does not grow well on LJ agar. It appears as a film after 3 to
4 weeks of incubation

A

M. avium

47
Q

appear as a “sun-spot” with starlike or asteroid margins a

A

M. avium

48
Q

rough colony has a “lacy” characteristic

A

M. avium

49
Q

have a strong yellow color; a positive catalase reaction, reduction of nitrate to nitrite, and rapid hydrolysis of Tween 80

A

M. kansasii on Middlebrook 7H11 agar.

50
Q

3-week-old colony growing on Middlebrook 7H11agar shows a dark, dense center and a wrinkled periphery,

A

Colony of M. kansasii

51
Q

does not reduce nitrates or assimilate iron, but it is suceptible to polymyxin B and resistant to ciprofloxacin.

A

M. chelonae

52
Q

grow on chocolate agar and appear as smooth. opaque colonies resembling staphylococci or yeast.

A

M. chelonae

53
Q

may appear as weakly staining. beaded, gram-positive bacilli, suggesting the possibility of a rapidly growing mycobacterium.

A

M. chelonae

54
Q

colonies appear dark and dense with smooth edges and a somewhat lighter center

A

M. chelonae

55
Q

can also grow on modifiedMac-Conkey agar, without crystal violet, at 37°C;

A

M. fortuitum

56
Q

Optimum temperature for M. fortuitum cultured in 7H11 & LJ

A

43 deg. Celsius

57
Q

a Runyon group II scotochromogens, characterized by yellow-orane pigment

A

M. gordonae

58
Q

Classify the Runyon group: M.smegmatis

A

Rapid growers

59
Q

Classify the Runyon group: M.thermoresistible

A

Scotochromogens

60
Q

Classify the Runyon group: M.terrae-triviale

A

Non-photochromogens

61
Q

Reagent strip impregnated with substrate cyanogen halide = yellow color

A

Niacin accumulation tests

62
Q

Base on the ability to convert ferric ammonium citrate to an iron oxide

A

Iron uptake test

63
Q

Growth at 28°C on an egg based medium containing 5% NaCl

A

NaCl tolerance test

64
Q

The only mycobacteria that is negative to NaCl Tolerance test

A

M.chelonae

65
Q

positive fot

A

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