MTAP (Introduction) Flashcards
Retention time of Records such as Requisition, Quality Control, and Instrument maintenance
2 years
Retention time of clinical pathology lab reports
2 years
Retention time of surgical pathology reports
10 years
Retention time of autopsy forensic reports
indefinitely
Retention time of cytogenetics reports
20 years
Retention time of bone marrow slides?
7 days
Retention time of cytogenetics slides
3 years
Retention time of pathology blocks
10 years
When accepting papers (2-3 papers) that contain information for the sample from OR they must include details such as:
- name of patient
- purpose of the surgery
- type of surgery
- type of tumor (if applicable)
- types of sutures
TAT of surgical pathology and cytology
5-7 days
TAT of PAP smear
- 3 days
TAT of frozen section
- 24 hours
Retention time of specimen
3 months
Retention time of tissue blocks
10 years
These are not diagnostic but only a guide in order to give initial diagnosis
Tumor markers
Retention time of slides
5-7 years
Sometimes mimic tumor marker, also the reason why TM is not diagnostic
Proteins
Criteria for malignancy
Chromatin in tissue
Tumor Marker associated with liver cancer and germ cell tumor
Alpha-fetoprotein (AFP)
Tumor Marker associated with ovarian cancer
CA-125
Tumor Marker associated with colorectal cancer
Carcinoembryonic antigen (CEA)
Tumor Marker associated with pancreatic, gallbladder, bile duct, and gastric cancers
CA19-9
Tumor Markers associated with breast cancer
- CA15-3/CA27.29
- Estrogen receptor (ER)/ progesterone receptor (PR)
Tumor Marker associated with B-cell lymphomas and leukemias
CD19
Tumor Marker associated with acute myeloid leukemia
CD33
Tumor Marker associated with chronic lymphocytic leukemia
Chromosome 17p deletion
Tumor Marker associated with non-hodgkin lymphoma
CD20
Tumor Marker associated with metastatic prostate cancer
Prostatic Acid Phosphatase (PAP)
Tumor Marker associated with colorectal cancer and non-small cell lung cancer
KRAS gene mutation
Acid Formaldehyde Hematin is removed by?
- Saturated alcoholic pictrate acid
- 1% alcoholic KOH
- Kardasewitsch MTDS
- Lillies’s MTDS
Tumor Marker associated with ovarian and breast cancers
BRCA1 and BRCA2 gene mutations
Tumor Marker associated with lymphomas and leukemias
BCL2 gene rearrangment
A healthy, relaxed, sedentary 70kg man who is killed instantly in an accident will usually have organs such as lungs, heart, liver, adrenals, thyroid, spleen, and brain weight in these ranges:
- Right Lung: 300-400 gm
- Left Lung: 250-350 gm
- Heart: 250-300 gm
- Liver: 1100-1600 gm
- Adrenals: 4 gm or so each
- Thyroid: 10-50 gm
- Spleen: 60-300 gm
- Brain: 1150-1450 gm
Appears as brown or black granules that may obscure micro details
Acid Formaldehyde Hematin
Appears as black granular deposits
Mercuric Chloride
Mercuric Chloride is removed by?
Alcoholic Iodine
Appears as black precipitate
Osmium tetroxide
Osmium tetroxide is removed by?
Cold water
A fine-yellow brown deposit
Chromate
Chromate is removed by?
Acid alcohol
Cheapest among blocks and pellets
Paraffin wax
Susbtitute for paraffin wax matter in the?
melting point (MP)
- Mixture of highly puried paraffin and synthetic plastic polymers
- It is more elastic and resilient than paraffin wax thereby permitting large dense tissue blocks such as bones and brain to be cut easily with the same result as in double embedding
Paraplast
MP of Paraplast
56-57C
- Soluble in common clearing agents and follows the same time schedule for paraffin impregnation
- Does not tend to crack like other paraffin wax substitutes
Paraplast
A semisynthetic wax recommended for embedding EYES
Bioloid
A synthetic wax substitute similar to Paraplast and is less brittle and less compressible than Paraplast
Embeddol
MP of Embeddol
56-58C
Clearing agents used for Ester wax
Cellosolve (ethylene glycol monoethyl ether) or xylene if indicated
A product of paraffin, containing RUBBER, with the same property as paraplast
Tissue mat
Its MP is around 46-48C, harder than paraffin, and is not soluble in water, but is soluble in 95% Ethyl Alc and other clearing agents
Ester wax
Has a MP 38-42C and are plastic polymers, mostly polyethylene glycols
Water-soluble waxes
Soluble and miscible with water. Hence, does not require dehydration and clearing tissue
Carbowax
A polyethylene glycol containing 18 or more carbon atoms, which appears solid at room temperature, the best wax but very expensive
Carbowax
An example of Polymer waxes (Water-Soluble Waxes)
Carbowax
Substitutes for Paraffin wax
- Paraplast
- Embeddol
- Bioloid
- Tissue mat
- Ester wax
- Water-soluble waxes
- Carbowax