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Immunohematology [Blood Banking] > MTAP 1 10/15/2022 > Flashcards

MTAP 1 10/15/2022 Flashcards

1
Q

gel test technology was developed by

A

Dr. Yves Lapierre in 1985

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2
Q

gel test technology: gel type

A

dextran polyacrylamide gel

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3
Q

gel test technology

microtube upper portion is

A

reaction of chamber

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4
Q

gel test technology

microtube lower portion is

A

column

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5
Q

gel test technology principle

A

size exclusion chromatography

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6
Q

type of gel tests

A
  1. specific
  2. plain / neutral
  3. low ionic antiglobulin
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7
Q

type of gel test specific for target antibody/antigen

A

specific

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8
Q

Type of gel test
- The gel is not yet dispensed with reagent
- Add the reagent along with the sample in the microtube

A

Plain / Neutral

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9
Q

gel test that do Reverse/Backward/Indirect/Serum Typing

A

plain / neutral

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10
Q

test gel specific for sensitization detection

A

low ionic antiglobulin

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11
Q

low ionic antiglobulin reagent added into the matrix

A

AHG reagent

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12
Q

Agglutination reaction in gel medium can be reviewed for _ DAYS (clump is stable)

A

3

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13
Q

gel test technology sample volume

A

<1ml

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14
Q

gel test:

The gel matrix itself traps the unbound _ that may interfere with the reaction

A

antibodies

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15
Q

grading of RBC most cells at the center portion in gel test

A

2+

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16
Q

grading of RBC near the bottom portion in gel test

A

weak (+/-)

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17
Q

antihuman globulin test is also known as

A

Coombs Test

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18
Q

end product of lattice formation

A

agglutination

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19
Q

best agglutinating antibody

A

IgM

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20
Q

Incomplete IgG antibodies are only capable of _ the red cell antigens.

A

sensitizing

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21
Q

principle of antihuman globulin test

A

detection of cell-bound immunoglobulin / incomplete antibodies (IgG)

22
Q

cold reacting antibody is best reactive at what temperature?

A

1-6deg C

23
Q

saline-reactive immunoglobulin

A

IgM

24
Q

albumin / ahg reactive immunoglobulin

A

IgG

25
Q

IgM ab example

IgG ab example

A

ABO

Rh

26
Q

IgG Most to least potent:

A

3,1,2

27
Q

type of AHG reagents

A

polyspecific AHG reagent
monospecific AHG reagent

28
Q

polyspecific ahg reagent is consist of

A
  • pool of rabbit anti-human IgG
  • mouse monoclonal anti-C3d and anti-C3b
29
Q

Polyspecific AHG Reagents are also refereed to

A

Broad Spectrum Coombs Reagent

30
Q

Add _ to distinguish AHG from other different reagent

A

green dye

31
Q

Method used for Monospecific reagent

A

Hybridoma Technique/Kohler and Milstein Technique

32
Q

cells combined in forming hybridoma cells

A

mouse plasma cells and myeloma cells

33
Q

stages of ab-ag interaction

A
  • sensitization
  • agglutination
34
Q

DAT detects _ sensitization

A

in vivo

35
Q

Immediate HTR is severe and fatal and usually
caused by

A

ABO incompatibility

36
Q

DAT collection tube

A

EDTA and citrate

37
Q

chelation of calcium holds _ subunits (_) together

A

C1; q,r,s

38
Q

largest complement protein

A

c1

39
Q

wharton’s jelly can cause

A

rouleaux formation

40
Q

presence of unbound antibodies can _ the AHG reagent resulting to

A

neutralize; false negative

41
Q

Delay in the addition of reagent = antibodies may
undergo

A

ELUTION PROCESS

42
Q

conventional method of DAT

A
  • washing
  • ahg addition
  • centrifugation
  • dislodge
  • observe
43
Q

minimum ration of serum to cells in AHG Test

A

40:1

44
Q

in AHG Test, drops of serum and cells

A

2 drops serum and 1 drop of 5% v/v cell suspension

45
Q

too high temperature in AHG Test can cause

A

protein denaturation

46
Q

too low temperature in AHG Test can cause

A

IgG inactivation

47
Q

in AHG Test incubation time in saline suspension

A

30-120 minutes

48
Q

in AHG Test incubation time in LISS suspension

A

10-15 minutes

49
Q

in AHG Test saline for washing; should be fresh and buffered to a pH of _

A

7.2-7.4

50
Q

in AHG test centrifugation time

A

1000 rcf for 15-20 seconds

Immunohematology [Blood Banking] (2 decks)

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