MT1 Flashcards

1
Q

what kind of environment do plants and animals prefer? (hypotonic, isotonic, hypertonic)

A

animals - isotonic; plants - hypotonic

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2
Q

at resting membrane potential, is the inside or outside of the cell relatively positive?

A

outside of the cell is relatively positive at resting membrane potential

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3
Q

T or F: diffusion needs no cell energy

A

T; diffusion doesn’t need cell energy

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4
Q

T or F: hydrogen bonds keep the temperature in oceans and cells constant

A

T: hydrogen bonds stabilize the water; each bond formation releases heat and prevents temperature from dropping further. Increased heat energy is absorbed by energy bonds.

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5
Q

T or F: H bonds prevent the formation of ionic bonds

A

T

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6
Q

Does water interact with non-polar molecules, like fats?

A

No

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7
Q

Describe surface tension of water (surface tension measures how hard it is to break the surface of something)

A

Water forms many H-bonds at the surface and just beneath the surface, making it hard to break the surface of the water

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8
Q

Glycogen and starch examples of ____saccharides

A

polysaccharides

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9
Q

T or F: a chloroblast is a type of amyloblast

A

T

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10
Q

Where can an ester linkage be found?

A

Between a fatty acid and a glycerol on a lipid molecule;

o-c=o

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11
Q

Fats take up _____space and more ______ energy than carbohydrates

A

Fats take up LESS space and more ENERGY than carbohydrates

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12
Q

Why are saturated fats more closely packed?

A

They have single C-C covalent bonds, so are very flexible and can pack closely

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13
Q

Why are unsaturated fats more rigid?

A

Their double C=C bonds produce a liquid, oily substance that doesn’t pack closely

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14
Q

Hydrophilic portions point towards the ____ portion of a membrane

A

Outer; they want to interact with water molecules. The hydrocarbon fatty-acid tail is non-polar and hydrophobic

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15
Q

What can proteins do?

A

1) speed up chem rxns (enzymes), 2) move in/out cell (membrane transporters) 3) support cells (cytoskeleton), 4) fight microbes (antibodies), 5) allow movement (shorten + contract muscles)

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16
Q

Can all amino acids be produced by the body?

A

No, 9 amino acids must be consumed

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17
Q

What are the 3 parts of a nucleotide?

A

1) deoxyribose sugar (5-C sugar) 2) -vely charged phosphate group on 5’-C of deoxyribose sugar, 3) nitrogenous base attached to the 1’-C of the deoxyribose sugar

18
Q

Enzymes add nucleotides to the ____ end of the DNA

A

Enzymes add nucleotides to the 3’ end of the DNA, copying them from 5’ to 3’

19
Q

Name some differences between RNA and DNA

A

DNA: larger, always double-stranded helix; deoxyribose (2’-C), GCAU

RNA: smaller, linear, oxyribose, GCAU, more versatile than DNA, enzymes

Both: store genetic information

20
Q

Why is RNA less stable than DNA?

A

OH group (RNA) is more EN than H group (DNA) and so the C-O (RNA) is more polar and less stable than the C-H (DNA)

21
Q

unzipping DNA involves breaking what kind of bonds?

A

H bonds between nitrogenous bases

22
Q

What is primase responsible for?

A

Primase is responsible for creating and attaching an RNA primer to the DNA strand (complementary base-pairs RNA nucleotides , then joins them together with a phosphodiester bond) - always between sugar and phosphate in backbone of any genetic material; writes from 5’ to 3’

23
Q

What does DNA polymerase do?

A

1) removes primer, fills gap with DNA nucleotides
2) attach to 3’ end, add nucleotides; when it sees a RNA primer, it detaches and re-attaches at junction to keep creating nucleotides
3) builds lagging and leading strands simultaneously, with a complex that has 2 DNA polymerases connected together

24
Q

What does DNA ligase do?

A

Connects DNA fragments - forming phosphodiester bonds between sugar and phosphate backbones

25
Why are telomeres important?
As the 'ends of DNA', they are short, non-coding segments that protect essential genes in middle of DNA. DNA polymerase needs a 3' end to add nucleotides (but when RNA primer is removed, there is no 3' end) - eventually DNA polymerase eats away at telomeres, then at essential genes, then the cells die. This is why we age!
26
What is PCR? Polymerase Chain Reaction? Name the steps.
1) method of making specific pieces of DNA 2-1) DNA is denatured using heat to break complementary strands (H-bonds) 2-2) Annealing; lab-created DNA primers is bound to ends of DNA that need to be copied 2-3) Extension; reheating the solution for DNA polymerase to lengthen the DNA primer in a 5' to 3' direction **each round of heating-cooling-heating doubles the amount of DNA to be copied each time**
27
How can you stop DNA sequencing?
Adding small amounts of a modified dideoxynucleotide can stop DNA polymerase and end replication - there is no 3' end in this modified nucleotide!!!
28
What is a plasmid?
Small, circular piece of DNA found in bacteria
29
What does a restriction enzyme do?
Cuts a plasmid (circular bacterial DNA) to allow for complementary extensions joined by DNA ligase -- result: recombinant DNA
30
How does CRISPR work?
1) RNA strand is made in lab, complementary to target DNA 2) RNA is linked to a protein, guides protein to the target DNA 3) protein cuts the target DNA 4) desired gene introduced 5) desired DNA is incorporated into its own genome during the repair process
31
What holds the plasma membrane together?
Van der waals interactions between fatty acids of phospholipid bilayer - allows membrane to move, and add/subtract things
32
What direction does fluidity affect?
The ability of proteins to move/drift sideways
33
Which portion of the membrane regulates passage?
The central, nonpolar region of the phospholipid bilayer decides what gets in/out - this is what decides the packing/spacing (permeability)
34
How does cholesterol buffer fluidity?
As a non-polar steroid, cholesterol creates space between fatty acids, prevents membrane from becoming solid, also fills in the middle nonpolar region when many gaps exist
35
A synthetic membrane composed of only phospholipids; organize from most easily to least easily penetrable (galactose, chloride ion, testosterone, atmospheric nitrogen, water, maltose)
atmospheric nitrogen (small, gaseous), testosterone (non-polar molecule), water (small molecule) galactose (monosaccharide), maltose (disaccharide) **chloride ions would need a channel to cross membrane**
36
How do plant cells adjust to changing environments in terms of membrane?
They use cholesterol to adjust the barrier, can adjust saturation/unsaturation of components
37
Is pinocytosis active transport?
Yes - it involves bringing something from a lower concentration to a higher concentration. Therefore, active transportation.
38
What concentration is most important to pay attention to in osmosis?
The free water concentration - the most solute you have, the less "free water" you have
39
A plant cell is surrounded by a dead cell wall. A plant cell would be unable to take in materials through... a) facilitated diffusion b) simple diffusion c) pinocytosis d) phagocytosis e) receptor-mediated endocytosis
d) phagocytosis
40
Do Van der Waal interactions happen between polar or nonpolar molecules?
nonpolar molecules - induced charge repulsion
41
How does cholesterol contribute to fluidity?
At high temperatures, cholesterol decreases fluidity. As a nonpolar molecule, it forms VDW interactions with neighbouring phospholipids, restricting their movement.
42
The presence of protein transporters affects ______ permeability
Selective Permeability; they move ions and polar molecules across the membrane; preventing charged particles from interacting with middle nonpolar portion of membrane