MST Flashcards

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1
Q

What are macromolecules?

A

Macromolecules are large groupings of many compounds, these then have different abilities depending on what the compounds are.

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2
Q

Examples of Macromolecules?
Properties of each example?

A

-Saccharides are carbs. These are called monosaccharides (glucose, fructose, galactose, ribose) or poly saccharides depending on how many there are. These are used as storage, cell signaling, digestive health and structuring.
-Proteins are another example, with a monomer of animo acids. These are used to make enzymes, transport, movement, communication.
-Nucleotides are what creates the DNA, they are codons which are used to tell the genetic makeup of the organism is. A monomer which are the A, T (U), C, G.
-Lipids, fatty acid, alcohol and phosphate group. Ester linkages. There is a hydrophilic and a hydrophobic end. Function as energy storage and chemical messengers. Fats, oils, waxes, hormones.

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3
Q

What is gene expression and how do you read it?

A

Gene expression is when the codons of the gene are expressed and therefore, we are able to see the exact nucleotides. Gene expression is also when DNA is transcribed into mRNA through RNA and then translated into a protein through ribosomes.

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4
Q

What are the 3 codon mutations that can occur?

A

The three codon mutations are when a nucleotide is either: removed or added to the sequence; changed into a different nucleotide; or a pre-emptive stop codon is formed.

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5
Q

What is DNA and RNA structure?

A

DNA is deoxyribose nucleic acid and RNA is ribose nucleic acid. There is one structural difference which changes the function of the nucleic acid, this is an oxygen on the 2 carbon of RNA. They are both made up of a phosphate group, sugar, nucleotide.

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6
Q

What is the soil microbiome?

A

The soil microbiome is the environment that is in the soil, this encompasses all the bacteria, archaea, fungi, Protists, viruses and it interacts with the nutrient cycling and soil structure.

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7
Q

What are the three domains?
Properties of each?

A

The three domains are:
-Eukaryotes: Have cell membrane; only some
have cell walls (e.g. plants [cellulose], fungi [chitin]), genetic material encased within nucleus, chromosomes are linear, unicellular or multicellular, have pathogenic species, Possess organelles.
-Archaea: Have a cell wall (polysaccharide)
and cell membrane, genetic material is free-floating within cytoplasm, circular chromosome, Unicellular, no known pathogenic species.
-Bacteria: Have a cell wall (peptidoglycan)
and cell membrane, genetic material is free-floating, Mithin cytoplasm, Circular chromosome, Unicellular, have pathogenic species.

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8
Q

What is Binary Fusion?

A

Binary fission is when the genetic material is duplicated and then the membrane elongates, then a wall is formed and then the parent cell becomes 2 daughter cells.

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9
Q

What is needed for cells to survive?

A

The right temperature, pH, nutrient availability, oxygen, growth factors

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10
Q

What are similarities between the 3 domains?

A

Has both DNA & RNA, Phospholipid membrane, Ribosomes & makes Proteins, has a cytoplasm.

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11
Q

How can Prokaryotes be used in everyday life?

A

Prokaryotes are used in food - for example yogurt and many dairy products- and medicine.

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12
Q

What is the Structure of Eukaryotic cells?

A

Eukaryotic cell has either a cell membrane or cell wall, they have membrane bound organelles, a nucleus, usually found in multicellular organisms.

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13
Q

Difference between plants and animal cells?

A

The main difference between plants and animal cells is that plants have cell walls and chloroplast whereas animal cells have cell walls and no chloroplasts.

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14
Q

What is the origin of the nucleus?

A

The nucleus it thought to be formed from the inwards folding of a nucleoid from a past prokaryote.

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15
Q

What is the origin of the mitochondria?

A

The mitochondria was a purple bacteria that ended up in a symbiotic relationship with the cell and has stated through reproduction.

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16
Q

What is the origin of the chloroplast?

A

The chloroplast was a cyanobacteria and was able to convert energy into food and not need energy through consumption and so in now still in plants.

17
Q

What is the Endomembrane system?

A

The endomembrane system is the system that is used to transport energy within the cell.

18
Q

Examples of organelles involved in the endomembrane system and their function.

A

Nucleus (holds the DNA ready for replication), Endoplasmic reticulum (protein synthesis and lipid metabolism), Golgi apparatus (receives the proteins and lipids and gets them ready to be shipped in vesicles to other areas of the cell or body), vesicles (what the proteins and lipids are shipped in).

19
Q

What is the cytoskeleton?
What makes up the cytoskeleton?

A

The cytoskeleton is made up of microtubules, intermediate filaments, and actin filaments that help shape the cell and the movement within.

20
Q

What is the Eukaryotic cell cycle?

A

The eukaryotic cell cycle is Growth–>DNA synthesis–>growth–>mitosis.

21
Q

What is the Eukaryotic cell division?

A

Mitosis: Interphase, Prophase, Prometaphase, Metaphase, Anaphase, Telophase.

22
Q

How do molecules move between cells?
Examples and properties.

A
  • Diffusion is the movement of particles from a high concentration to a low concentration.
    ○ Simple diffusion is when molecules move freely though a semipermeable membrane, non-polar and small
    ○ Passive facilitated transport is when the particles are assisted by transport proteins
    ○ Primary active transport is when larger molecules use pumps to get through, these pumps use ATP to help go against the gradient.
    ○ Secondary active transport is when a secondary pump uses excess energy from the primary pump to pump more molecules across the membrane.
    § Example of passive: glucose going into cells when exercising
    § Example of active: glucose getting pumped out of cells after eating sugar
23
Q

What is concentration gradient?

A

Concentration gradient is the amount of concentrate on each side of the membrane and where the concentrate wants to move to

24
Q

What is water potential?

A

Water potential is the amount of free water molecules in an area, the water wants to not be free.

25
Q

What is Photosynthesis?
Where does it take place?
What organelles does it use?

A
  • Photosynthesis is the creation of energy from light
    ○ This occurs in the chloroplast more specifically in the thylakoid
26
Q

What is the light dependent reactions?

A
  • Light dependent reaction: light is accepted by the photosystems which increases their energy, this excites the P680 which then expels an electron, this then creates and unstable P680, a water molecule is broken and O2 is now a byproduct, the electron is accepted by the electron accepter and not the P680 is stable. This leads to the electron transport chain which moves energy through protein through redox reaction and the excess H+ ion gets expelled through these reactions, this energy that is being transferred now becomes NADPH ready for the Calvin cycle to use. Because of the expelling of a H+ ion there is now a protein gradient which initialises the synthesis of ATP.
27
Q

What are light independent reactions?

A

Light independent creates three carbon sugars from CO2, this is called carbon fixation.

28
Q

What is cellular respiration?

A

Cellular respiration is what cells use to receive energy to keep the cell alive

29
Q

What are the stages in Cellular respiration?

A

Glycolysis (happens in the cytoplasm, breaking down of glucose) –> pyruvate oxidation (transported into the mitochondria, oxidized into acetic acid) –> citric acid cycle ()–> electron transfer.

30
Q

How is energy stored in glucose transformed into temporary electron carriers?

A

When pyruvate is formed though the splitting of glucose, it generates ATP and NADPH which are high energy electron carriers

31
Q

How is energy stored in the temporary electron carriers is converted to electron-potential energy in the electron transport chain?

A

Energy in ATP and NADPH form moves through the electron chain by redox reactions which creates energy for the cell to use.

32
Q

How does proton motive cause ATP synthesis?

A

The energy made during redox reactions is used to expels H+ ions which creates a proton gradient within the mitochondria, this then causes ATP synthesis to balance out the gradient.

33
Q

What are
Chromatin, Centrosome, Kinetochore, Spindle, Microtubules, Centromere, Chromatid, Chromosome

A

Chromatin - DNA which is packaged, or in complex, with histone proteins in the nucleus
Centrosome - A region near the nucleus containing centrioles (in animal cells), from which spindle fibres form
Kinetochore - A complex of proteins which associate with the centromere and allow spindle fibres to attach
Spindle - The structure formed from two asters, consisting of long fibres of microtubules which extend from the centrosome.
Microtubules - reach out from the spindle and connect to the kinetochore, and also push apart the two poles of the cell using motor proteins
Centromere - Region at the centre of a chromosome which allows sister chromatids to pair and mitotic spindle to attach
Chromatid - One strand of a newly replicated chromosome, connected by the centromere
Chromosome - A single double stranded DNA molecule, bound with histones as chromatin, which has condensed prior to cell division.