Moughamian... Holzbaur. J. Neur.

Question 0

EBs

Answer 0

End-binding proteins. These recognize a GTP-tubulin-related structural conformation enriched at the ends of dynamically growing microtubules and recruit proteins with either 1. A CAP-Gly domain or 2. A SxIP motif - to the microtubule plus end.

Question 1

CLIPS

Answer 1

Cytoplasmic linker proteins. They have a CAP-Gly domain which binds to 1. the C-terminus of alpha-tubulin, 2. EBs - EB1 and EB3.

• CLIP-170
• CLIP-115

Question 2

+TIPS (name 5 examples)

Answer 2

The name given to microtubule plus-end tracking proteins (in the paper).

1. Proteins with CAP-Gly
2. Proteins with SxIP
3. EBs
4. CLIPS
5. Lis1

Question 3

Lis1

Answer 3

Another +TIP, and a dynein regulator. 4 things to know about lis:

1. Dynein regulator
2. Associated with lissencephaly
3. Associated with neuronal migration
4. Associated with retrograde transport

Question 4

Dynactin

Answer 4

Enhances dynein processivity, mediates cargo interactions, promotes efficiency of retrograde transport, necessary for transport initiation in Drosophila

Question 5

What is the overall goal of this paper?

Answer 5

Investigate the role of dynamic microtubules and +TIPS in initiation of dynein-mediated retrograde axonal transport.