MORPHOLOGIC ABNORMALITIES OF LEUKOCYTES Flashcards
characterized by granulocytes (monocyte and lymphocyte) with large darkly staining metachromatic cytoplasm granules
ALDER-REILY ANOMALY
ALDER REILY ANOMALY - autosomal dominant or autosomal recessive
AUTOSOMAL RECESSIVE
granulation, partially digested mucopolysaccharide
REILLY BODIES
prominent dark granulation, either fine or heavy = can be observe in band and segmented neutrophil or monocytes
TOXIC GRANULATION
represents an increase in acid mucosubstance with azurophilic (primary) granules
peroxidase positive
more than five lobes or nuclear segmentation
hypersegmentation
hypersegmentation is associated with deficiencies in what
Vit. B12 and Folic Acid
Hypersegmentation - Autosomal dominant or autosomal recessive
autosomal dominant
decrease nuclear segmentation
Pelger-Huet Anomaly
Pelger-Huet Anomaly -Autosomal dominant or autosomal recessive
Autosomal dominant
Shape of nuclei of Pelger-Huet Anomaly
round, ovoid, or peanut
Pelger-Huet Anomaly is a result from a mutation of what gene
lamin B-receptor gene
TRUE or FALSE - Neutrophils functions normally in Pelger-Huet Anomaly
TRUE
nuclear chromatin condenses, segments disappear, dark staining spheres, apoptotic nucleus, dying neutrophils
Pyknotic Nucleus
spectacle-like (pince-nez) morphology is found in what
Pelger-Huet Anomaly
light blue, sharply define crescent or round shape inclusion, giant platelet, usually single dohle-like body inclusion, but may be multiple
May Hegglin Anomaly
large hypogranular platelets and thrombocytopenia
May Hegglin Anomaly
seen near the periphery of the cytoplasm
Dohle Bodies
Dohle Bodies represents aggregates of what
Rough Endoplasmic Reticulum (RNA)
intracytoplasmic pale blue or elongated inclusion located close to cellular membranes
Dohle Bodies
group of more than 50 inherited enzyme deficiencies resulting from mutations in genes that code for the production of lysosomal enzyme
Lysosomal Storage Disease
flawed degradation of phagocytized material and buildup of undigested substrates within lysosomes
Lysosomal Storage Disease
AKA Barr Body/X or Sex Chromatin
Pyknotic Nucleus
darkly stained structure in the nucleus most often found in the periphery of the nucleus
Pyknotic Nucleus
caused by deficient activity of an enzyme necessary for the degradation of dermatan sulfate, heparan sulfate, keratan sulfate, and/or chondroitin sulfate
Mucopolysaccharisoses
B-glucosidase(glucocerebrosidase) si available ot confirm diagnosis
GAUCHER DISEASE
GAUCHER DISEASE treatment
enzyme replacement therapy with recombinant glucocerebrosidase
Accumulation of FAT ni celular lysosomes of vital organs
NEIMANN-PICK DISEASE
NEIMANN-PICK DISEASE is a Recessive mutations in what gene?
SMPD1 gene
- Adutl form of Niemann pick disease anchdronic granulocytic leukemia
- Histiocytes filed with LIPID RICH GRANULES
- BLUE GREEN with Polychrome stain suchsa Giemsa or Wright Stain
SEA BLUE HISTIOCYTES
hyper segmentation is often associated with deficiency of
Vit. B12 and folic acid
associated with infectious states such as burns, malignant disorders,or as a result of drug therapy
toxic granulation
WBC AFFECTED IN TOXIC GRANULATION
neutrophils
WBC AFFECTED IN ARA
neutrophils, monocytes, lymphocytes
Toxic granulation or ARA
associated with neutrophilia with left shift
Toxic Granulation
toxic Granulation or ARA
not associated with dohle bodies
ARA
difference between PHA and myelocytes/metamyelocytes
PHA cell size smaller
PHA n:c ratio is lower
PHA chromatic is darker, more coarse,more densely clumped
PHA have colorless cytoplasm
Myelocytes have cytoplasm basophilia and neutrophilic left shift
Niemann-Pick Disease is positive in what stain
sudan black b, oil red o
macrophage with lipid filled lysosomes that appears as small vacuoles; nucleus is eccentric
Foam cells
Gaucher’s Diesease is positive in what stain
PAS, trichrome, aldehyde fuchsin, acid phosphatase
auer rods form cluster
faggots cells
suggestive of promyelocytic leukemia
faggots cells
may hegglin anomaly is mutation in what gene
MYH9 gene on chromosome 22q12-13
