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Metabolism
all chemical reactions occurring in an organism
Anabolism
chemical reactions that expend energy to make new chemical bonds
Catabolism
chemical reactions that harvest energy when bonds are broken
cofactors
usually metal ions, found in the active site participating in catalysis
coenzymes
nonprotein organic molecules, often used as an electron donor or acceptor in a redox reaction
Energy
the capacity to do work
kinetic energy
the energy of motion
potential energy
stored energy
Energy can take many forms
mechanical electric current
heat light
Heat energy is measured in
kilocalories.
One calorie
= the amount of heat required to raise the temp of water by 1oC
oxidation:
loss of electrons
reduction
gain of electrons
redox reactions
are coupled to each other
NAD+
- is an electron carrier
- NAD accepts 2 electrons and 1 proton to become NADH
- the reaction is reversible
aerobic respiration
final electron receptor is oxygen (O2)
anaerobic respiration
final electron acceptor is an inorganic molecule (not O2)
fermentation
final electron acceptor is an organic molecule
substrate-level phosphorylation
transferring a phosphate directly to ADP from another molecule
oxidative phosphorylation
use of ATP synthase and energy derived from a proton (H+) gradient to make ATP
The complete oxidation of glucose proceeds in stages
- glycolysis
- pyruvate oxidation
- Krebs cycle
- electron transport chain & chemiosmosis
Glycolysis
converts glucose to pyruvate.
- a 10-step biochemical pathway
- occurs in the cytoplasm
- 2 molecules of pyruvate are formed
- net production of 2 ATP molecules by substrate-level phosphorylation
- 2 NADH produced by the reduction of NAD+
For glycolysis to continue, NADH must be recycled to NAD+ by either:
- aerobic respiration – occurs when oxygen is available as the final electron acceptor
- fermentation – occurs when oxygen is not available; an organic molecule is the final electron acceptor
Pyruvate Oxidation
In the presence of oxygen, pyruvate is oxidized.
- occurs in the mitochondria in eukaryotes
- occurs at the plasma membrane in prokaryotes
- in mitochondria, a multienzyme complex called pyruvate dehydrogenase catalyzes the reaction