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1
Q

Metabolism

A

all chemical reactions occurring in an organism

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2
Q

Anabolism

A

chemical reactions that expend energy to make new chemical bonds

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3
Q

Catabolism

A

chemical reactions that harvest energy when bonds are broken

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4
Q

cofactors

A

usually metal ions, found in the active site participating in catalysis

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5
Q

coenzymes

A

nonprotein organic molecules, often used as an electron donor or acceptor in a redox reaction

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6
Q

Energy

A

the capacity to do work

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7
Q

kinetic energy

A

the energy of motion

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8
Q

potential energy

A

stored energy

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9
Q

Energy can take many forms

A

mechanical electric current

heat light

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10
Q

Heat energy is measured in

A

kilocalories.

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11
Q

One calorie

A

= the amount of heat required to raise the temp of water by 1oC

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12
Q

oxidation:

A

loss of electrons

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13
Q

reduction

A

gain of electrons

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14
Q

redox reactions

A

are coupled to each other

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15
Q

NAD+

A
  • is an electron carrier
  • NAD accepts 2 electrons and 1 proton to become NADH
  • the reaction is reversible
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16
Q

aerobic respiration

A

final electron receptor is oxygen (O2)

17
Q

anaerobic respiration

A

final electron acceptor is an inorganic molecule (not O2)

18
Q

fermentation

A

final electron acceptor is an organic molecule

19
Q

substrate-level phosphorylation

A

transferring a phosphate directly to ADP from another molecule

20
Q

oxidative phosphorylation

A

use of ATP synthase and energy derived from a proton (H+) gradient to make ATP

21
Q

The complete oxidation of glucose proceeds in stages

A
  1. glycolysis
  2. pyruvate oxidation
  3. Krebs cycle
  4. electron transport chain & chemiosmosis
22
Q

Glycolysis

A

converts glucose to pyruvate.

  • a 10-step biochemical pathway
  • occurs in the cytoplasm
  • 2 molecules of pyruvate are formed
  • net production of 2 ATP molecules by substrate-level phosphorylation
  • 2 NADH produced by the reduction of NAD+
23
Q

For glycolysis to continue, NADH must be recycled to NAD+ by either:

A
  1. aerobic respiration – occurs when oxygen is available as the final electron acceptor
  2. fermentation – occurs when oxygen is not available; an organic molecule is the final electron acceptor
24
Q

Pyruvate Oxidation

A

In the presence of oxygen, pyruvate is oxidized.

  • occurs in the mitochondria in eukaryotes
  • occurs at the plasma membrane in prokaryotes
  • in mitochondria, a multienzyme complex called pyruvate dehydrogenase catalyzes the reaction
25
Q

Krebs cycle

A

oxidizes the acetyl group from pyruvate.
-occurs in the matrix of the mitochondria
-biochemical pathway of 9 steps
-first step:
acetyl group + oxaloacetate citrate
(2 carbons) (4 carbons) (6 carbons)

26
Q

The remaining steps of the Krebs cycle

A
  • release 2 molecules of CO2
  • reduce 3 NAD+ to 3 NADH
  • reduce 1 FAD (electron carrier) to FADH2
  • produce 1 ATP
  • regenerate oxaloacetate
27
Q

After glycolysis, pyruvate oxidation, and the Krebs cycle, glucose has been oxidized to

A
  • 6 CO2
  • 4 ATP
  • 10 NADH
  • 2 FADH2
28
Q

electron transport chain (ETC)

A

is a series of membrane-bound electron carriers.

  • embedded in the mitochondrial inner membrane
  • electrons from NADH and FADH2 are transferred to complexes of the ETC
  • each complex transfers the electrons to the next complex in the chain
29
Q

Electron Transport Chain

A

The higher negative charge in the matrix attracts the protons (H+) back from the intermembrane space to the matrix.

The accumulation of protons in the intermembrane space drives protons into the matrix via diffusion.

30
Q

Most protons move back to the matrix through

A

ATP synthase

31
Q

ATP synthase

A

is a membrane-bound enzyme that uses the energy of the proton gradient to synthesize ATP from ADP + Pi.

32
Q

Chloroplasts

A

organelles present in cells of plants and some other eukaryotes

- contain chlorophyll for photosynthesis
- surrounded by 2 membranes
33
Q

thylakoids

A

are membranous sacs within the inner membrane

34
Q

grana

A

are stacks of thylakoids

35
Q

Mitochondria and chloroplasts:

A
  • have 2 membranes
    • possess DNA and ribosomes
    • are about the size of a prokaryotic cell
    • divide by a process similar to bacteria