monoclonal antibodies

Question 1

What are monoclonal antibodies

Answer 1

● Antibodies, made from the white blood cell lymphocytes, that are clones from one
parent cell
● very specific to one type of antigen (anything foreign)

Question 2

Describe how monoclonal antibodies are

produced

Answer 2

1. Specific antigen injected into an animal (e.g. mouse).
2. B-lymphocytes producing complementary antibodies extracted.
3. B-lymphocytes fuse with myeloma cells to form hybridoma cells - these cells can divide and produce antibodies.
4. Hybridoma cells cultured.
5. Monoclonal antibodies collected and purified.

Question 3

Outline the uses of monoclonal

antibodies

Answer 3

● Detection of pathogens
● Location of cancer cells and blood clots
● Treatment of cancer
● Used in pregnancy test kits

Question 4

What are myeloma cells?

Answer 4

Type of tumour cell

Question 5

What do pregnancy kits test for?

Answer 5

hCG in urine

Question 6

What does a pregnancy test consist of?

Answer 6

A stick containing monoclonal antibodies (mAbs) specific to hCG:
● mAbs attached to a blue bead (free to move)
● mAbs fixed to the test stick

Question 7

Describe what happens to the test stick if

a woman is pregnant

Answer 7

● hCG in urine binds to mAbs attached to
a blue bead.
● mAbs with hCG diffuse up dipstick.
● mAbs fixed to the stick bind to hCG.
● Blue line forms.

Question 8

Describe what happens to the test stick if

a woman is not pregnant

Answer 8

No hCG in urine so a blue line is not

formed.

Question 9

What is the advantage of using
monoclonal antibodies to test for
pathogens?

Answer 9

● Specific to one particular antigen
● Very accurate
● Quick results

Question 10

Why can monoclonal antibodies be used

to target cancer cells?

Answer 10

● Cancer cells have specific antigens called ‘tumour markers’ on their membranes.
● mAbs are specific to one type of antigen so can be targeted to ‘tumour markers’ without damaging other cells.

Question 11

Describe how monoclonal antibodies can

be used to diagnose cancer

Answer 11

● mAbs tagged to a radioactive substance.
● mAbs injected into the patient’s bloodstream.
● mAbs bind to ‘tumour markers’ on cancer cells.
● Emitted radiation is detected using a specialised scanner enabling doctors to determine the location of cancer cells.

Question 12

How can monoclonal antibodies be used

to target drugs to cancer cells?

Answer 12

● mAbs attached to an anti-cancer drug.
● mAbs injected into the patient’s bloodstream.
● mAbs bind to ‘tumour markers’ on cancer cells.
● Anti-cancer drug destroys cancer cells.

Question 13

Why are cancer treatments that use
monoclonal antibodies favoured over
traditional treatments?

Answer 13

● Radiotherapy and chemotherapy target rapidly dividing cells.
● Healthy cells (e.g. hair follicle cells, bone marrow cells) are damaged as a consequence, producing unpleasant side
effects.
● mAbs only target cancer cells, reducing damage to normal cells.

Question 14

How can monoclonal antibodies be used

to locate blood clots?

Answer 14

● mAbs tagged to a radioactive substance.
● mAbs target and bind to specific proteins in blood clots.
● Radiation emitted by mAbs is detected, enabling the location of blood clots to be identified.