Molecular Studies and Flow In Lymphoma

Question 1

Indications for southern blot

Answer 1

When PCR not possible/too expensive

Detect monoclonal AgR re arrangements that are missed in PCR assays

Question 2

Adv of southern blot

Answer 2

Low FP and FN rate

Question 3

Disadvantage of SB

Answer 3

Fresh tissue required
Time
Dear and labour
Radioactive materials
Low analytic sensitivity - ie limited utility in specimens with low proportions of monoclonal cells

Question 4

Normal germinal centre B cell expression

Answer 4

CD 10 + and bcl2-ve

Aberrant expression of these tow together

Question 5

Abnormal bcl 2 expression

Answer 5

Most FL
Some DLBCL
Some B lineage ALL

Question 6

CD5+\10-

Answer 6

CLL, SLL MCL
Some w B PLL
Remember CD 5 is expressed in small proportion of B cells which are normally in blood so needs to be interpreted w caution

Question 7

CD 5- \ CD10 +

Answer 7

DLBCL, FL
CD 10 expression in other lymphoma is rare
Also childhood BL
Small subset HC are CD 10

Question 8

Cd5-/cd10-

Answer 8

Diverse group- cd10-fl, Mzl, DLBCL,LPL, HCL

MZL is usually CD 10- and differentiate from HC, MCL and cd10- FL by morphology

Question 9

CD5 and CD10+ve

Answer 9

Rare

DLBCL, FL, MCL, CLL, BL