Molecular pathology Flashcards

1
Q

What are techniques of molecular pathology?

A
In situ hybridization
Polymerase chain reaction
Restriction fragment length polymorphism
Northern blot
Southern blot
Western blot
Flow cytometry
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2
Q

What is in situ hybridization?

A

study of cells content

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3
Q

What is PCR?

A

amplification of DNA/RNA

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4
Q

What is restriction fragmentation length polymorphism

A

gene deletions are insertion in disease

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5
Q

What is Northern blot?

A

gene exprssion

detects RNA

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6
Q

What is Southern blot?

A

detects DNA sequence

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7
Q

What is western blot?

A

antibodies to separated proteins

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8
Q

What is flow cytometry

A

cell surface markers

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9
Q

What is laboratory clinical medicine divided into?

A
Immunohematology (blood banking)
Chemistry
Hematology
Urinalysis
Coagulation
Serology
Microbiology
Molecular 
cytogenetics
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10
Q

What does pathology encompass about a disease?

A

all aspects of a disease

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11
Q

What are all aspects of a disease?

A
Cause (etiology)
Mechanism of development (pathogenesis) 
Alteration in cells and organs 
      Morphologic/pathologic changes
Functional consequences (clinical signs & symptom)
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12
Q

What are the etiology (causes) of diseases?

A

Genetic (e.g., Mendelian inherited mutations)

Acquired (e.g., infectious, nutritional, chemical, physical).

Disease-associated gene variants (polymorphisms) & environment

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